Skip to main content
PMC home page
The Journal of Biophysical and Biochemical Cytology logoLink to The Journal of Biophysical and Biochemical Cytology
. 1960 Apr 1;7(2):345–352. doi: 10.1083/jcb.7.2.345

Electron Microscope Studies of Nuclear Extrusions in Pancreatic Acinar Cells of the Rat

Wallace H Clark Jr 1
PMCID: PMC2224796  PMID: 13810485

Abstract

This paper describes "blebs" protruding from the surface of the nucleus into the cytoplasm. The "blebs" are separated from the cytoplasm by 2 membranes which are continuous with the outer and inner nuclear membranes. The "blebs" contain 3 structurally distinct substances. Two of these substances (β and γ substances) are similar to extranucleolar karyoplasm and nucleolar material. The other substance (α substance) is present in every "bleb," but it cannot be readily compared to a recognizable nuclear structure. Cytoplasmic vesicles are described that are apparently different from the Golgi vesicles or the vesicular component of the ergastoplasm. It is suggested that these vesicles may be of nuclear "bleb" origin. A dark karyoplasmic zone extending from the region of the nucleolus into the nuclear "bleb" is shown. This zone may be similar in some respects to the preformed pathway ("Leitbahn") described by Altmann (3) and Hertl (28) and could reflect movement of nuclear material from the nucleolar region into the cytoplasm. The "blebs" are thought to be homologous to structures described by many light microscopists, but they are considerably larger than the nuclear "blebs" described previously by electron microscopists.

Full Text

The Full Text of this article is available as a PDF (1.3 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. AFZELIUS B. A. Electron microscopy of Golgi elements in sea urchin eggs. Exp Cell Res. 1956 Aug;11(1):67–85. doi: 10.1016/0014-4827(56)90191-4. [DOI] [PubMed] [Google Scholar]
  2. AFZELIUS B. A. Electron microscopy on the basophilic structures of the sea urchin egg. Z Zellforsch Mikrosk Anat. 1957;45(6):660–675. doi: 10.1007/BF00338710. [DOI] [PubMed] [Google Scholar]
  3. ALTMANN H. W. Uber den Funktionsformwechsel des Kernes im exokrinen Gewebe des Pankreas. Z Krebsforsch. 1952;58(6):632–645. [PubMed] [Google Scholar]
  4. ALTMANN H. W. Zur Morphologie der Wechselwirkung von Kern und Cytoplasma. Klin Wochenschr. 1955 Apr 1;33(13-14):306–314. doi: 10.1007/BF01467837. [DOI] [PubMed] [Google Scholar]
  5. ANDERSON E., BEAMS H. W. Evidence from electron micrographs for the passage of material through pores of the nuclear membrane. J Biophys Biochem Cytol. 1956 Jul 25;2(4 Suppl):439–444. doi: 10.1083/jcb.2.4.439. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. BAUD C. A. Recherches sur la structure de la membrane nucleaire. Acta Anat (Basel) 1953;17(2):113–174. [PubMed] [Google Scholar]
  7. CALLAN H. G., TOMLIN S. G. Experimental studies on amphibian oocyte nuclei. I. Investigation of the structure of the nuclear membrane by means of the electron microscope. Proc R Soc Lond B Biol Sci. 1950 Oct 13;137(888):367–378. doi: 10.1098/rspb.1950.0047. [DOI] [PubMed] [Google Scholar]
  8. Caspersson T., Schultz J. Ribonucleic Acids in Both Nucleus and Cytoplasm, and the Function of the Nucleolus. Proc Natl Acad Sci U S A. 1940 Aug 15;26(8):507–515. doi: 10.1073/pnas.26.8.507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. DE ROBERTIS E. The nucleo-cytoplasmic relationship and the basophilic substance (ergastoplasm) of nerve cells; electron microscope observations. J Histochem Cytochem. 1954 Sep;2(5):341–345. doi: 10.1177/2.5.341. [DOI] [PubMed] [Google Scholar]
  10. FICQ A. Etude autoradiographique dumétabolisme des protéines et des acides nucléiques au cours de l'oogénèse chez les batraciens. Exp Cell Res. 1955 Oct;9(2):286–293. doi: 10.1016/0014-4827(55)90101-4. [DOI] [PubMed] [Google Scholar]
  11. GAY H. Chromosome-nuclear membrane-cytoplasmic interrelations in Drosophila. J Biophys Biochem Cytol. 1956 Jul 25;2(4 Suppl):407–414. [PMC free article] [PubMed] [Google Scholar]
  12. HAGUENAU F., BERNHARD W. Particularités structurales de la membrane nucléaire; étude au microscope électronique de cellules normales et cancéreuses. Bull Assoc Fr Etud Cancer. 1955;42(5):537–544. [PubMed] [Google Scholar]
  13. HORSTMANN E., KNOOP A. Zur Struktur des Nucleolus und des Kernes. Z Zellforsch Mikrosk Anat. 1957;46(1):100–107. [PubMed] [Google Scholar]
  14. LAIRD A. K., BARTON A. D., NYGAARD O. Synthesis of protein and ribonucleic acid in rat liver during refeeding after starvation. Exp Cell Res. 1955 Dec;9(3):523–540. doi: 10.1016/0014-4827(55)90081-1. [DOI] [PubMed] [Google Scholar]
  15. MOSES M. J. Studies on nuclei using correlated cytochemical, light, and electron microscope techniques. J Biophys Biochem Cytol. 1956 Jul 25;2(4 Suppl):397–406. doi: 10.1083/jcb.2.4.397. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. PALADE G. E. A study of fixation for electron microscopy. J Exp Med. 1952 Mar;95(3):285–298. doi: 10.1084/jem.95.3.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. REBHUN L. I. Electron microscopy of basophilic structures of some invertebrate oocytes. II. Fine structure of the yolk nuclei. J Biophys Biochem Cytol. 1956 Mar 25;2(2):159–170. doi: 10.1083/jcb.2.2.159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. SWIFT H. The fine structure of annulate lamellae. J Biophys Biochem Cytol. 1956 Jul 25;2(4 Suppl):415–418. doi: 10.1083/jcb.2.4.415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. WATSON M. L. Further observations on the nuclear envelope of the animal cell. J Biophys Biochem Cytol. 1959 Oct;6:147–156. doi: 10.1083/jcb.6.2.147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. WATSON M. L. Staining of tissue sections for electron microscopy with heavy metals. II. Application of solutions containing lead and barium. J Biophys Biochem Cytol. 1958 Nov 25;4(6):727–730. doi: 10.1083/jcb.4.6.727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. WATSON M. L. The nuclear envelope; its structure and relation to cytoplasmic membranes. J Biophys Biochem Cytol. 1955 May 25;1(3):257–270. doi: 10.1083/jcb.1.3.257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. WENDT E. Lebendbeobachtungen an bestrahlten Interphasekernen. Z Zellforsch Mikrosk Anat. 1959;49(6):677–689. [PubMed] [Google Scholar]
  23. WISCHNITZER S. An electron microscope study of the nuclear envelope of amphibian oocytes. J Ultrastruct Res. 1958 Apr;1(3):201–222. doi: 10.1016/s0022-5320(58)80001-5. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of Biophysical and Biochemical Cytology are provided here courtesy of The Rockefeller University Press

RESOURCES