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. 1960 Apr 1;7(2):273–282. doi: 10.1083/jcb.7.2.273

Some Characteristics of DNA Synthesis and the Mitotic Cycle in Ehrlich Ascites Tumor Cells

Joshua L Edwards 1, Arthur L Koch 1, Pauline Youcis 1, Herbert L Freese 1, Melville B Laite 1, J Thomas Donalson 1
PMCID: PMC2224797  PMID: 13819420

Abstract

In vivo studies of Ehrlich ascites tumor cells during the first 5 days of growth in peritoneal cavities of mice consisted of the following: 1. Determination of growth curves by direct enumeration of cells. 2. Estimation of the duration of each phase of the mitotic cycle based on incidence of cells in different phases. 3. Radioautographic studies to determine the proportion of cells in different phases of the mitotic cycle that incorporate tritiated thymidine during a single brief exposure to this precursor of DNA. 4. Estimation of the rate of incorporation of tritiated thymidine at different times during the period of DNA synthesis by comparison of mean grain counts over nuclei in radioautographs at different times following exposure to tritiated thymidine. The assumptions underlying these experiments and our observations concerning the duration of the period of DNA synthesis and its relation to the mitotic cycle are discussed. It is concluded that DNA synthesis is continuous, occupying a period of 8.5 hours during the interphase and that the average rate of synthesis is approximately constant.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DAVIDSON J. N., SMELLIE R. M., KEIR H. M., McARDLE A. H. Synthesis of deoxyribonucleic acid in extracts of mammalian cells. Nature. 1958 Aug 30;182(4635):589–590. doi: 10.1038/182589a0. [DOI] [PubMed] [Google Scholar]
  2. FRIEDKIN M., TILSON D., ROBERTS D. Studies of deoxyribonucleic acid biosynthesis in embryonic tissues with thymidine-C14. J Biol Chem. 1956 Jun;220(2):627–637. [PubMed] [Google Scholar]
  3. HORNSEY S., HOWARD A. Autoradiograph studies with mouse Ehrlich ascites tumor. Ann N Y Acad Sci. 1956 Mar 14;63(5):915–928. doi: 10.1111/j.1749-6632.1956.tb50900.x. [DOI] [PubMed] [Google Scholar]
  4. Hughes W. L., Bond V. P., Brecher G., Cronkite E. P., Painter R. B., Quastler H., Sherman F. G. CELLULAR PROLIFERATION IN THE MOUSE AS REVEALED BY AUTORADIOGRAPHY WITH TRITIATED THYMIDINE. Proc Natl Acad Sci U S A. 1958 May;44(5):476–483. doi: 10.1073/pnas.44.5.476. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. KLEIN G., KLEIN E. The viability and the average desoxypentosenucleic acid content of micronuclei-containing cells produced by colchicine treatment in the Ehrlich ascites tumor. Cancer Res. 1952 Jul;12(7):484–489. [PubMed] [Google Scholar]
  6. KLEIN G., REVESZ L. Quantitative studies on the multiplication of neoplastic cells in vivo. I. Growth curves of the Ehrlich and MC1M ascites tumors. J Natl Cancer Inst. 1953 Oct;14(2):229–277. doi: 10.1093/jnci/14.2.229. [DOI] [PubMed] [Google Scholar]
  7. LAJTHA L. G. Detection of adenine carbon-14 in deoxyribonucleic acid by autoradiography. Nature. 1954 Mar 27;173(4404):587–588. doi: 10.1038/173587a0. [DOI] [PubMed] [Google Scholar]
  8. LAJTHA L. G., OLIVER R., ELLIS F. Incorporation of 32P and adenine 14C into DNA by human bone marrow cells in vitro. Br J Cancer. 1954 Jun;8(2):367–379. doi: 10.1038/bjc.1954.38. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. PASTEELS J., LISON L. Recherches histophotométriques sur la teneur en acide désoxyribosenucléique au cours de mitoses somatiques. Arch Biol (Liege) 1950;61(3):445–474. [PubMed] [Google Scholar]
  10. PATT H. M., BLACKFORD M. E., DRALLMEIER J. L. Growth characteristics of the Krebs ascites tumor. Proc Soc Exp Biol Med. 1953 Jul;83(3):520–524. doi: 10.3181/00379727-83-20403. [DOI] [PubMed] [Google Scholar]
  11. REICHARD P., ESTBORN B. Utilization of desoxyribosides in the synthesis of polynucleotides. J Biol Chem. 1951 Feb;188(2):839–846. [PubMed] [Google Scholar]
  12. RICHARDS B. M. Deoxyribose nucleic acid values in tumour cells with reference to the stem-cell theory of tumour growth. Nature. 1955 Feb 5;175(4449):259–261. doi: 10.1038/175259a0. [DOI] [PubMed] [Google Scholar]
  13. RICHARDS B. M., WALKER P. M., DEELEY E. M. Changes in nuclear DNA in normal and ascites tumor cells. Ann N Y Acad Sci. 1956 Mar 14;63(5):831–848. doi: 10.1111/j.1749-6632.1956.tb50895.x. [DOI] [PubMed] [Google Scholar]
  14. RITTER H. B. A simple permanent aceto-orcein stain for free cells, cultures and homogenates. Stain Technol. 1958 Jul;33(4):167–170. doi: 10.3109/10520295809111842. [DOI] [PubMed] [Google Scholar]
  15. SCHERBAUM O., RASCH G. Cell size distribution and single cell growth in Tetrahymena pyriformis GL. Acta Pathol Microbiol Scand. 1957;41(3):161–182. doi: 10.1111/j.1699-0463.1957.tb01014.x. [DOI] [PubMed] [Google Scholar]
  16. Taylor J. H., Woods P. S., Hughes W. L. THE ORGANIZATION AND DUPLICATION OF CHROMOSOMES AS REVEALED BY AUTORADIOGRAPHIC STUDIES USING TRITIUM-LABELED THYMIDINEE. Proc Natl Acad Sci U S A. 1957 Jan 15;43(1):122–128. doi: 10.1073/pnas.43.1.122. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. WALKER P. M. B., YATES H. B. Nuclear components of dividing cells. Proc R Soc Lond B Biol Sci. 1952 Oct 16;140(899):274–299. doi: 10.1098/rspb.1952.0062. [DOI] [PubMed] [Google Scholar]

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