Abstract
Bacillus polymyxa sporangia and spores were prepared for examination in the electron microscope by methods whose critical features were apparently: judicious use of vacuum, to encourage complete penetration of the embedding medium; the use of epoxy resins as embedding media; and cutting of the thin sections with a diamond knife. Electron micrographs of material prepared in this manner exhibit undeformed sporangial sections. Some of the structures revealed have been shown before, though perhaps less distinctly; other structures are revealed here for the first time. While this single study does not pretend to elucidate all the complexities of sporulation in bacteria, these and similar images should make this possible, and some mention of the preparatory techniques that lead to them seems advisable at this time.
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Selected References
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- ANINGA S., VAN DEN HOOFF A. An electron microscope study on the shape of the spores of Bacillus polymyxa. Antonie Van Leeuwenhoek. 1956;22(4):327–330. doi: 10.1007/BF02538345. [DOI] [PubMed] [Google Scholar]
- BIRCH-ANDERSEN A., MAALØE O., SJOSTRAND F. S. High-resolution electron micrographs of sections of E. coli. Biochim Biophys Acta. 1953 Nov;12(3):395–400. doi: 10.1016/0006-3002(53)90157-7. [DOI] [PubMed] [Google Scholar]
- CHAPMAN G. B. Electron microscopy of ultra-thin sections of bacteria. II. Sporulation of Bacillus megaterium and Bacillus cereus. J Bacteriol. 1956 Mar;71(3):348–355. doi: 10.1128/jb.71.3.348-355.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GLAUERT A. M., GLAUERT R. H., ROGERS G. E. A new embedding medium for electron microscopy. Nature. 1956 Oct 13;178(4537):803–803. doi: 10.1038/178803a0. [DOI] [PubMed] [Google Scholar]
- HASHIMOTO T., NAYLOR H. B. Studies of the fine structure of microorganisms. II. Electron microscopic studies on sporulation of Clostridium sporogenes. J Bacteriol. 1958 Jun;75(6):647–653. doi: 10.1128/jb.75.6.647-653.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROBINOW C. F. Spore structure as revealed by thin sections. J Bacteriol. 1953 Sep;66(3):300–311. doi: 10.1128/jb.66.3.300-311.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
- TOKUYASU K., YAMADA E. Fine structure of Bacillus subtilis. II. Sporulation progress. J Biophys Biochem Cytol. 1959 Jan 25;5(1):129–134. doi: 10.1083/jcb.5.1.129. [DOI] [PMC free article] [PubMed] [Google Scholar]