Abstract
Healthy, mature, spontaneously contracting muscle was cultivated from explants of 13-day chick embryos for periods up to 4 months in the multipurpose chamber (Rose, 1954) using cellophane-strip technique (Rose et al., 1958) with silicone gaskets, Eagle's medium including 10 per cent horse serum reinforced with 300 mg-per cent of glucose, and the teased type of explant. This method provided optically ideal conditions for the study of muscle fibers with oil immersion, phase contrast time-lapse cinematography at 1 frame per minute without apparent damage for periods as long as 10 days. In no case was mitosis, amitosis, or nuclear "budding" observed in the course of muscle development. Multinuclear muscle fibers have been shown with cine technique to result from both myoblast fusion and polar extension of preformed (explanted) muscle tissue. Myoblast fusion was the only demonstrable way of giving rise to multinucleation. Nuclear membrane "wrinkling" was shown to be merely a temporary distortion that occurred during nuclear migration and rotation. It is suggested that this phenomenon may be responsible for numerous reports of amitosis in the genesis of muscle fibers. The histological development of new straps resulted from an orderly sequence of events. Included in these were polar extension, nuclear migration, rotation, and fixation. Following these events there was increased mitochondrial activity, myofibril formation, and cross-banding. Spontaneous contractions were seen throughout the entire course of differentiation in vitro but became more regular and stronger in the later stages.
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Selected References
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- GODMAN G. C., MURRAY M. R. Influence of colchicine on the form of skeletal muscle in tissue culture. Proc Soc Exp Biol Med. 1953 Dec;84(3):668–672. doi: 10.3181/00379727-84-20746. [DOI] [PubMed] [Google Scholar]
- GODMAN G. C. The effect of colchicine on striated muscle in tissue culture. Exp Cell Res. 1955 Jun;8(3):488–499. doi: 10.1016/0014-4827(55)90125-7. [DOI] [PubMed] [Google Scholar]
- HOLTZER H., MARSHALL J. M., Jr, FINCK H. An analysis of myogenesis by the use of fluorescent antimyosin. J Biophys Biochem Cytol. 1957 Sep 25;3(5):705–724. doi: 10.1083/jcb.3.5.705. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LASH J. W., HOLTZER H., SWIFT H. Regeneration of mature skeletal muscle. Anat Rec. 1957 Aug;128(4):679–697. doi: 10.1002/ar.1091280404. [DOI] [PubMed] [Google Scholar]
- MOSCONA A. Development of heterotypic combinations of dissociated embryonic chick cells. Proc Soc Exp Biol Med. 1956 Jun;92(2):410–416. doi: 10.3181/00379727-92-22495. [DOI] [PubMed] [Google Scholar]
- POMERAT C. M. Cinematographic analysis of cell dynamics. Fed Proc. 1958 Dec;17(4):975–984. [PubMed] [Google Scholar]
- ROSE G. G., POMERAT C. M., SHINDLER T. O., TRUNNELL J. B. A cellophane-strip technique for culturing tissue in multipurpose culture chambers. J Biophys Biochem Cytol. 1958 Nov 25;4(6):761–764. doi: 10.1083/jcb.4.6.761. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROSE G. A separable and multipurpose tissue culture chamber. Tex Rep Biol Med. 1954;12(4):1074–1083. [PubMed] [Google Scholar]