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. 1978 Sep;135(3):1070–1079. doi: 10.1128/jb.135.3.1070-1079.1978

Transconjugant analysis: limitations on the use of sequence-specific endonucleases for plasmid identification.

S C Causey, L R Brown
PMCID: PMC222484  PMID: 357414

Abstract

We used the sequence-specific endonucleases EcoRI, SmaI, BamHI, HsuI, and HaeIII as identification tools in following the conjugal transfer of the well-studied R plasmids Sa, R388, RP4, and R6K. Transfers were both intergeneric and intrageneric. Plasmid fingerprints were generated from both single- and combination-enzyme digests. The Sa transconjugants yielded plasmids showing consistent fingerprints for each of the respective endonucleases used, whereas the three other R-plasmid transconjugants showed fingerprint changes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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