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. 1960 Jul 1;7(4):603–612. doi: 10.1083/jcb.7.4.603

The Relationship of Internal Conductance and Membrane Capacity to Mitochondrial Volume

Helmut Pauly 1, Lester Packer 1
PMCID: PMC2224882  PMID: 14431036

Abstract

A study was made of the effect of mitochondrial size on the electrical properties of the membrane and the internal conductivity of mitochondria. The dielectric constant and electrical conductivity of suspensions of guinea pig heart mitochondria were examined in the frequency range 5 x 105 to 2.5 x 108 C.P.S. Membrane capacity was calculated to be 1.1 to 1.3 µf./cm.2 and was virtually the same in mitochondria whose surface area was made to vary by a factor of 4 by osmotic means. This finding suggested that some mechanism must exist for the transfer of mitochondrial material into membrane structure during fluctuations in mitochondrial size. The electrical capacity of the membrane was unaffected by a 33-fold change in potassium chloride concentration. The internal conductance of swollen mitochondria was 2 to 3 times lower than that of the external medium. In shrunken mitochondria the internal conductance was virtually independent of the conductivity of the external medium. These results were discussed in relation to current concepts of mitochondrial structure.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. CLELAND K. W., SLATER E. C. Respiratory granules of heart muscle. Biochem J. 1953 Mar;53(4):547–556. doi: 10.1042/bj0530547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. DAVIES R. E., FONNESU A., PRICE C. A. Movements of water and ions in mitochondria. Biochem J. 1956 Dec;64(4):754–768. doi: 10.1042/bj0640754. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. FAWCETT D. W., ITO S. Observations on the cytoplasmic membranes of testicular cells, examined by phase contrast and electron microscopy. J Biophys Biochem Cytol. 1958 Mar 25;4(2):135–142. doi: 10.1083/jcb.4.2.135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. HODGKIN A. L., KEYNES R. D. The mobility and diffusion coefficient of potassium in giant axons from Sepia. J Physiol. 1953 Mar;119(4):513–528. doi: 10.1113/jphysiol.1953.sp004863. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. HUNTER F. E., Jr, DAVIS J., CARLAT L. The stability of oxidative and phosphorylative systems in mitochondria under anaerobic conditions. Biochim Biophys Acta. 1956 Apr;20(1):237–242. doi: 10.1016/0006-3002(56)90282-7. [DOI] [PubMed] [Google Scholar]
  6. LEHNINGER A. L., RAY B. L. Oxidation-reduction state of rat liver mitochondria and the action of thyroxine. Biochim Biophys Acta. 1957 Dec;26(3):643–644. doi: 10.1016/0006-3002(57)90115-4. [DOI] [PubMed] [Google Scholar]
  7. LEVER J. D., CHAPPELL J. B. Mitochondria isolated from rat brown adipose tissue and liver. J Biophys Biochem Cytol. 1958 May 25;4(3):287–290. doi: 10.1083/jcb.4.3.287. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. PALADE G. E. An electron microscope study of the mitochondrial structure. J Histochem Cytochem. 1953 Jul;1(4):188–211. doi: 10.1177/1.4.188. [DOI] [PubMed] [Google Scholar]
  9. PAULY H. Electrical conductance and dielectric constant of the interior of erythrocytes. Nature. 1959 Jan 31;183(4657):333–334. doi: 10.1038/183333a0. [DOI] [PubMed] [Google Scholar]
  10. PAULY H., PACKER L., SCHWAN H. P. Electrical properties of mitochondrial membranes. J Biophys Biochem Cytol. 1960 Jul;7:589–601. doi: 10.1083/jcb.7.4.589. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. REVEL J. P., ITO S., FAWCETT D. W. Electron micrographs of myelin figures of phospholipide simulating intracellular membranes. J Biophys Biochem Cytol. 1958 Jul 25;4(4):495–498. doi: 10.1083/jcb.4.4.495. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. ROBERTSON J. D. Structural alterations in nerve fibers produced by hypotonic and hypertonic solutions. J Biophys Biochem Cytol. 1958 Jul 25;4(4):349–364. doi: 10.1083/jcb.4.4.349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. SIEKEVITZ P., WATSON M. L. The isolation and analysis of a mitochondrial membrane fraction. J Biophys Biochem Cytol. 1956 Jul 25;2(4 Suppl):379–382. doi: 10.1083/jcb.2.4.379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. WITTER R. F., WATSON M. L., COTTONE M. A. Morphology and ATP-ase of isolated mitochondria. J Biophys Biochem Cytol. 1955 Mar;1(2):127–138. doi: 10.1083/jcb.1.2.127. [DOI] [PMC free article] [PubMed] [Google Scholar]

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