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. 1960 Sep 1;8(1):135–150. doi: 10.1083/jcb.8.1.135

THE STRIATED MUSCULATURE OF BLOOD VESSELS

II. Cell Interconnections and Cell Surface

H E Karrer 1
PMCID: PMC2224914  PMID: 13751592

Abstract

The interconnections and the surfaces of the striated muscle cells which occur in thoracic and in lung veins of the mouse were studied with the electron microscope. The osmium-fixed tissues were embedded in methacrylate or in araldite and sectioned with a Porter-Blum microtome. Many preparations were stained before embedding with phosphotungstic acid or after sectioning with uranyl acetate. Typical intercalated discs are observed in this muscle. They are similar to the discs found in heart muscle. These intercalated discs represent boundaries between separate muscle cells. Along the discs, cells are joined in planes normal to their myofilaments. The same cells are also joined in planes parallel to the myofilaments by means of lateral interconnections. These lateral cell boundaries are in continuity with the intercalated discs. Three morphologically distinct parts occur within the lateral cell interconnections: One is characterized by small vesicles along the plasma membrane, the second part has the structure of desmosomes, and a third part represents an external compound membrane (formed by the two plasma membranes of the adjoining cells) and is termed "quintuple-layered cell interconnection." Small vesicles and plasma membrane enfoldings along the free surface of muscle cells are interpreted as products of a pinocytosis (phagocytosis) process. Some of them are seen to contain small membrane-bounded bodies or granules. The free cell surface shows a characteristic outer dense layer ("basement membrane") which accompanies the plasma membrane. The topographic relation of this dense layer with the plasma membrane seems to vary in different preparations. The significance of this variation is not well understood. On two occasions a typical arrangement o vesicles and tubules was observed at Z band levels, just beneath the plasma membrane. These structures are believed to represent endoplasmic reticulum. Their possible significance for the conduction of excitation is discussed.

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Selected References

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  1. EKHOLM R., SJOSTRAND F. S. The ultrastructural organization of the mouse thyroid gland. J Ultrastruct Res. 1957 Dec;1(2):178–199. doi: 10.1016/s0022-5320(57)80006-9. [DOI] [PubMed] [Google Scholar]
  2. FAWCETT D. W., SELBY C. C. Observations on the fine structure of the turtle atrium. J Biophys Biochem Cytol. 1958 Jan 25;4(1):63–72. doi: 10.1083/jcb.4.1.63. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. HUXLEY A. F., TAYLOR R. E. Local activation of striated muscle fibres. J Physiol. 1958 Dec 30;144(3):426–441. doi: 10.1113/jphysiol.1958.sp006111. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. KARRER H. E. Cell interconnections in normal human cervical epithelium. J Biophys Biochem Cytol. 1960 Feb;7:181–184. doi: 10.1083/jcb.7.1.181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. MOORE D. H., RUSKA H. Electron microscope study of mammalian cardiac muscle cells. J Biophys Biochem Cytol. 1957 Mar 25;3(2):261–268. doi: 10.1083/jcb.3.2.261. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. MOORE D. H., RUSKA H. The fine structure of capillaries and small arteries. J Biophys Biochem Cytol. 1957 May 25;3(3):457–462. doi: 10.1083/jcb.3.3.457. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. MUIR A. R. An electron microscope study of the embryology of the intercalated disc in the heart of the rabbit. J Biophys Biochem Cytol. 1957 Mar 25;3(2):193–202. doi: 10.1083/jcb.3.2.193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. PEACHEY L. D., PORTER K. R. Intracellular impulse conduction in muscle cells. Science. 1959 Mar 13;129(3350):721–722. doi: 10.1126/science.129.3350.721. [DOI] [PubMed] [Google Scholar]
  9. PEASE D. C. Electron microscopy of the vascular bed of the kidney cortex. Anat Rec. 1955 Apr;121(4):701–721. doi: 10.1002/ar.1091210402. [DOI] [PubMed] [Google Scholar]
  10. POCHE R., LINDNER E. Untersuchungen zur Frage der Glanzstreifen des Herzmuskelgewebes beim Warmblüter und beim Kaltblüter. Z Zellforsch Mikrosk Anat. 1955;43(2):104–120. [PubMed] [Google Scholar]
  11. RHODIN J. Electron microscopy of the glomerular capillary wall. Exp Cell Res. 1955 Jun;8(3):572–574. doi: 10.1016/0014-4827(55)90136-1. [DOI] [PubMed] [Google Scholar]
  12. ROBERTSON J. D. New observations on the ultrastructure of the membranes of frog peripheral nerve fibers. J Biophys Biochem Cytol. 1957 Nov 25;3(6):1043–1048. doi: 10.1083/jcb.3.6.1043. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. ROBERTSON J. D. Some features of the ultrastructure of reptilian skeletal muscle. J Biophys Biochem Cytol. 1956 Jul 25;2(4):369–380. doi: 10.1083/jcb.2.4.369. [DOI] [PubMed] [Google Scholar]
  14. ROBERTSON J. D. Structural alterations in nerve fibers produced by hypotonic and hypertonic solutions. J Biophys Biochem Cytol. 1958 Jul 25;4(4):349–364. doi: 10.1083/jcb.4.4.349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. ROBERTSON J. D. The ultrastructure of cell membranes and their derivatives. Biochem Soc Symp. 1959;16:3–43. [PubMed] [Google Scholar]
  16. RUSKA H., EDWARDS G. A., CAESAR R. A concept of intracellular transmission of excitation by means of the endoplasmic reticulum. Experientia. 1958 Mar 15;14(3):117–120. doi: 10.1007/BF02159249. [DOI] [PubMed] [Google Scholar]
  17. SJOSTRAND F. S., ANDERSSON E. Electron microscopy of the intercalated discs of cardiac muscle tissue. Experientia. 1954 Sep 15;10(9):369–370. doi: 10.1007/BF02160542. [DOI] [PubMed] [Google Scholar]
  18. WATSON M. L. Further observations on the nuclear envelope of the animal cell. J Biophys Biochem Cytol. 1959 Oct;6:147–156. doi: 10.1083/jcb.6.2.147. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. WATSON M. L. Staining of tissue sections for electron microscopy with heavy metals. J Biophys Biochem Cytol. 1958 Jul 25;4(4):475–478. doi: 10.1083/jcb.4.4.475. [DOI] [PMC free article] [PubMed] [Google Scholar]

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