Abstract
Colonies and spore suspensions of Streptomyces coelicolor were fixed for electron microscopy by the method of Kellenberger, Ryter, and Séchaud (1958). In thin sections the nuclear regions have a lower average density than the cytoplasm and the outlines of these regions correspond well with the profiles of the chromatinic bodies observed with the light microscope. The nuclear regions contain fibrils, about 5 mµ in diameter. In contrast, after fixation by the method of Palade (1952) the nuclear material is coagulated into irregular dense masses and tubular structures about 20 mµ in diameter, lying in a nuclear "vacuole." The significance of these observations is discussed in relation to the observations of other workers on the fine structure of the nuclear material of other bacteria and the chromosomes of higher cells.
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Selected References
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- CHAPMAN G. B., KROLL A. J. Electron microscopy of ultrathin sections of Spirillum serpens. J Bacteriol. 1957 Jan;73(1):63–71. doi: 10.1128/jb.73.1.63-71.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DE D. N., KAUFMANN B. P. Fine structure of chromosomes. J Biophys Biochem Cytol. 1956 Jul 25;2(4 Suppl):419–424. doi: 10.1083/jcb.2.4.419. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GLAUERT A. M., HOPWOOD D. A. A membranous component of the cytoplasm in Streptomyces coelicolor. J Biophys Biochem Cytol. 1959 Dec;6:515–516. doi: 10.1083/jcb.6.3.515. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GLAUERT A. M., HOPWOOD D. A. The fine structure of Streptomyces coelicolor. I. The cytoplasmic membrane system. J Biophys Biochem Cytol. 1960 Jun;7:479–488. doi: 10.1083/jcb.7.3.479. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GRELL K. G., WOHLFARTH-BOTTERMANN K. E. Licht- und elektronenmikroskopische Untersuchungen an dem Dinoflagellaten Amphidinium elegans n. sp. Z Zellforsch Mikrosk Anat. 1957;47(1):7–17. [PubMed] [Google Scholar]
- HOPWOOD D. A., GLAUERT A. M. Observations on the chromatinic bodies of Streptomyces coelicolor. J Biophys Biochem Cytol. 1960 Sep;8:257–265. doi: 10.1083/jcb.8.1.257. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KAUFMANN B. P., MCDONALD M. R. Organization of the chromosome. Cold Spring Harb Symp Quant Biol. 1956;21:233–246. doi: 10.1101/sqb.1956.021.01.019. [DOI] [PubMed] [Google Scholar]
- PALADE G. E. A study of fixation for electron microscopy. J Exp Med. 1952 Mar;95(3):285–298. doi: 10.1084/jem.95.3.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RIS H. A study of chromosomes with the electron microscope. J Biophys Biochem Cytol. 1956 Jul 25;2(4 Suppl):385–392. doi: 10.1083/jcb.2.4.385. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RYTER A., KELLENBERGER E., BIRCHANDERSEN A., MAALOE O. Etude au microscope électronique de plasmas contenant de l'acide désoxyribonucliéique. I. Les nucléoides des bactéries en croissance active. Z Naturforsch B. 1958 Sep;13B(9):597–605. [PubMed] [Google Scholar]
- STUART D. C., Jr Fine structure of the nucleoid and internal membrane systems of Streptomyces. J Bacteriol. 1959 Aug;78:272–281. doi: 10.1128/jb.78.2.272-281.1959. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WILKINS M. H., ZUBAY G. The absence of histone in the bacterium Escherichia coli. II. X-ray diffraction of nucleoprotein extract. J Biophys Biochem Cytol. 1959 Jan 25;5(1):55–58. doi: 10.1083/jcb.5.1.55. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ZUBAY G., WATSON M. R. The absence of histone in the bacterium Escherichia coli. I. Preparation and analysis of nucleoprotein extract. J Biophys Biochem Cytol. 1959 Jan 25;5(1):51–54. doi: 10.1083/jcb.5.1.51. [DOI] [PMC free article] [PubMed] [Google Scholar]