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The Journal of Biophysical and Biochemical Cytology logoLink to The Journal of Biophysical and Biochemical Cytology
. 1960 Oct 1;8(2):399–412.

ETUDE AU MICROSCOPE ELECTRONIQUE DES TRANSFORMATIONS NUCLEAIRES DE E. COLI K12 S ET K12 S (λ26) APRES IRRADIATION AUX RAYONS ULTRAVIOLETS ET AUX RAYONS X

Antoinette Ryter 1
PMCID: PMC2224942  PMID: 13745076

Abstract

Nuclear transformations induced in E. coli K12S and K12S(λ26) by ultraviolet radiations and x-rays have been studied with ultrathin sections in the electron microscope. The nucleoplasm keeps its normal aspect during "fragmentation" and during "condensation" of the nucleus into the "vesicular" form. Serial sections show that the "fragmented" nucleus consists in reality of only one very tortuous vacuole. No difference either in the shape or in the fine structure of the nucleus could be observed between the lysogenic strain and the non-lysogenic strain. A high concentration of NaCl has a "condensation" effect on the fragmented nuclei and decreases the induction rate.

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Selected References

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  1. BARNER H. D., COHEN S. S. The relation of growth to the lethal damage induced by ultraviolet irradiation in Escherichia coli. J Bacteriol. 1956 Feb;71(2):149–157. doi: 10.1128/jb.71.2.149-157.1956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CEDERGREN B., HOLME T. On the glycogen in Escherichia coli B; electron microscopy of ultrathin sections of cells. J Ultrastruct Res. 1959 Oct;3:70–73. doi: 10.1016/s0022-5320(59)80016-2. [DOI] [PubMed] [Google Scholar]
  3. HAROLD F. M., ZIPORIN Z. Z. Effect of nitrogen and sulfur mustard on nucleic acid synthesis in Escherichia coli. Biochim Biophys Acta. 1958 Jun;28(3):482–491. doi: 10.1016/0006-3002(58)90509-2. [DOI] [PubMed] [Google Scholar]
  4. HAROLD F. M., ZIPORIN Z. Z. Synthesis of protein and of DNA in Escherichia coli irradiated with ultraviolet light. Biochim Biophys Acta. 1958 Aug;29(2):439–440. doi: 10.1016/0006-3002(58)90211-7. [DOI] [PubMed] [Google Scholar]
  5. HARTMAN P. E., PAYNE J. I., MUDD S. Cytological analysis of ultraviolet irradiated Escherichia coli. I. Cytology of lysogenic E. coli and a non-lysogenic derivative. J Bacteriol. 1955 Nov;70(5):531–539. doi: 10.1128/jb.70.5.531-539.1955. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. JACOB F., WOLLMAN E. L. Analyse des groupes de liaison génétique de différentes souches donatrices d'Escherichia coli K 12. C R Hebd Seances Acad Sci. 1957 Nov 18;245(21):1840–1843. [PubMed] [Google Scholar]
  7. KELLENBERGER E., RYTER A. Contribution à l'étude du noyau bactérien. Schweiz Z Pathol Bakteriol. 1955;18(5):1122–1137. [PubMed] [Google Scholar]
  8. KELLENBERGER E., SECHAUD J., RYTER A. Electron microscopical studies of phage multiplication. IV. The establishment of the DNA pool of vegetative phage and the maturation of phage particles. Virology. 1959 Aug;8:478–498. doi: 10.1016/0042-6822(59)90050-9. [DOI] [PubMed] [Google Scholar]
  9. KELNER A. Growth, respiration, and nucleic acid synthesis in ultraviolet-irradiated and in photoreactivated Escherichia coli. J Bacteriol. 1953 Mar;65(3):252–262. doi: 10.1128/jb.65.3.252-262.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Meselson M., Stahl F. W. THE REPLICATION OF DNA IN ESCHERICHIA COLI. Proc Natl Acad Sci U S A. 1958 Jul 15;44(7):671–682. doi: 10.1073/pnas.44.7.671. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. RYTER A., KELLENBERGER E., BIRCHANDERSEN A., MAALOE O. Etude au microscope électronique de plasmas contenant de l'acide désoxyribonucliéique. I. Les nucléoides des bactéries en croissance active. Z Naturforsch B. 1958 Sep;13B(9):597–605. [PubMed] [Google Scholar]
  12. RYTER A., KELLENBERGER E. L'inclusion au polyester pour l'ultramicrotomie. J Ultrastruct Res. 1958 Dec;2(2):200–214. doi: 10.1016/s0022-5320(58)90018-2. [DOI] [PubMed] [Google Scholar]
  13. WATSON M. L. Staining of tissue sections for electron microscopy with heavy metals. II. Application of solutions containing lead and barium. J Biophys Biochem Cytol. 1958 Nov 25;4(6):727–730. doi: 10.1083/jcb.4.6.727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. WEIGLE J. J., DELBRUCK M. Mutual exclusion between an infecting phage and a carried phage. J Bacteriol. 1951 Sep;62(3):301–318. doi: 10.1128/jb.62.3.301-318.1951. [DOI] [PMC free article] [PubMed] [Google Scholar]

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