Skip to main content
The Journal of Biophysical and Biochemical Cytology logoLink to The Journal of Biophysical and Biochemical Cytology
. 1960 Oct 1;8(2):305–318. doi: 10.1083/jcb.8.2.305

THE ULTRASTRUCTURE OF CARDIAC DESMOSOMES IN THE TOAD AND THEIR RELATIONSHIP TO THE INTERCALATED DISC

Philip M Grimley 1, George A Edwards 1
PMCID: PMC2224943  PMID: 13708707

Abstract

The fine structure of desmosomes and intercalated discs in the toad heart is discussed. A definite relationship between the dense components of these structures and the dense region of the Z band is demonstrated. The dense region of the Z band characteristically widens at its approach to the plasma membrane, and often terminates beneath it in a distinct discoidal plaque. Cardiac desmosomes appear to be structures which result from the intimate apposition of plaques of Z band material. These desmosomes retain the Z band function as sites of attachment for myofilaments. The suggestion is made that rotation of a desmosome through 90° and splitting of filaments from the adjacent sarcomere could result in the formation of a simple step-like intercalated disc. Intermediate stages in this process are illustrated. Complex discs present in the toad probably represent the alignment of groups of simple discs produced by contractile forces. Possible physiologic functions of the disc and desmosome are discussed. Other morphologic features of toad cardiac cells include a distinct amorphous outer coat to the sarcolemma, a prominent N band, and a granular sarcoplasm with poorly developed reticulum.

Full Text

The Full Text of this article is available as a PDF (1.6 MB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARRNETT R. J., PALADE G. E. Enzymatic activity in the M band. J Biophys Biochem Cytol. 1959 Oct;6:163–170. doi: 10.1083/jcb.6.2.163. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BOURNE G. H. Enzymes of the intercalated disks of heart muscle fibres. Nature. 1953 Sep 26;172(4378):588–589. doi: 10.1038/172588a0. [DOI] [PubMed] [Google Scholar]
  3. FAWCETT D. W., SELBY C. C. Observations on the fine structure of the turtle atrium. J Biophys Biochem Cytol. 1958 Jan 25;4(1):63–72. doi: 10.1083/jcb.4.1.63. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. MOORE D. H., GRIMLEY P. H. Problems in methacrylate embedding for electron microscopy. J Biophys Biochem Cytol. 1957 Mar 25;3(2):255–260. doi: 10.1083/jcb.3.2.255. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. MOORE D. H., RUSKA H. Electron microscope study of mammalian cardiac muscle cells. J Biophys Biochem Cytol. 1957 Mar 25;3(2):261–268. doi: 10.1083/jcb.3.2.261. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. MUIR A. R. An electron microscope study of the embryology of the intercalated disc in the heart of the rabbit. J Biophys Biochem Cytol. 1957 Mar 25;3(2):193–202. doi: 10.1083/jcb.3.2.193. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. ODLAND G. F. The fine structure of the interrelationship of cells in the human epidermis. J Biophys Biochem Cytol. 1958 Sep 25;4(5):529–538. [PMC free article] [PubMed] [Google Scholar]
  8. POCHE R., LINDNER E. Untersuchungen zur Frage der Glanzstreifen des Herzmuskelgewebes beim Warmblüter und beim Kaltblüter. Z Zellforsch Mikrosk Anat. 1955;43(2):104–120. [PubMed] [Google Scholar]
  9. PORTER K. R., PALADE G. E. Studies on the endoplasmic reticulum. III. Its form and distribution in striated muscle cells. J Biophys Biochem Cytol. 1957 Mar 25;3(2):269–300. doi: 10.1083/jcb.3.2.269. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. RUSKA H., EDWARDS G. A., CAESAR R. A concept of intracellular transmission of excitation by means of the endoplasmic reticulum. Experientia. 1958 Mar 15;14(3):117–120. doi: 10.1007/BF02159249. [DOI] [PubMed] [Google Scholar]
  11. SJOSTRAND F. S., ANDERSSON-CEDERGREN E., DEWEY M. M. The ultrastructure of the intercalated discs of frog, mouse and guinea pig cardiac muscle. J Ultrastruct Res. 1958 Apr;1(3):271–287. doi: 10.1016/s0022-5320(58)80008-8. [DOI] [PubMed] [Google Scholar]
  12. SJOSTRAND F. S., ANDERSSON E. Electron microscopy of the intercalated discs of cardiac muscle tissue. Experientia. 1954 Sep 15;10(9):369–370. doi: 10.1007/BF02160542. [DOI] [PubMed] [Google Scholar]
  13. VAN BREEMEN V. L. Intercalated discs in heart muscle studied with the electron microscope. Anat Rec. 1953 Sep;117(1):49–63. doi: 10.1002/ar.1091170106. [DOI] [PubMed] [Google Scholar]
  14. WOOD R. L. Intercellular attachment in the epithelium of Hydra as revealed by electron microscopy. J Biophys Biochem Cytol. 1959 Dec;6:343–352. doi: 10.1083/jcb.6.3.343. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Biophysical and Biochemical Cytology are provided here courtesy of The Rockefeller University Press

RESOURCES