Abstract
The effect of x-irradiation on grasshopper spermatogenesis was studied with the aid of light and electron microscopy. The insects were irradiated at the second instar prior to the presence of maturation stages and observed at the last instar and imago stages. Dosages of 100 to 600 roentgens were found to retard the differentiation of the nucleus and mitochondrial nebenkern in spermatids. Evidence is presented that irradiation causes a curtailment and disorganization in the differentiation of the nebenkern from mitochondria. The above doses also induced the formation of supernumerary centrioles, flagellar filaments and acrosomes; nuclear disorganization as well as pycnosis and fragmentation also occur. The nucleus appears to be drawn toward each radiation-induced supernumerary acrosome, with consequent multipolarity of the nucleus. Induction of a set of flagellar filaments is seen only where the centriolar structure is in contact with the nucleus. Details are given of an organelle, heretofore not described, that is composed of anastomosed and interwoven cytoplasmic strands.
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Selected References
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- DE ROBERTIS E., FRANCO RAFFO H. Submicroscopic organization of the mitochondrial body and other cytoplasmic structures of insect testis. Exp Cell Res. 1957 Feb;12(1):66–79. doi: 10.1016/0014-4827(57)90294-x. [DOI] [PubMed] [Google Scholar]
- DEVINE R. L., POWERS E. L., TAHMISIAN T. N. Light and electron microscope studies of morphological changes of mitochondria during spermatogenesis in the grasshopper. J Biophys Biochem Cytol. 1956 Jul 25;2(4 Suppl):325–330. doi: 10.1083/jcb.2.4.325. [DOI] [PMC free article] [PubMed] [Google Scholar]
- YASUZUMI G., ISHIDA H. Spermatogenesis in animals as revealed by electron microscopy. II. Submicroscopic structure of developing spermatid nuclei of grasshopper. J Biophys Biochem Cytol. 1957 Sep 25;3(5):663–668. doi: 10.1083/jcb.3.5.663. [DOI] [PMC free article] [PubMed] [Google Scholar]