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The Journal of Biophysical and Biochemical Cytology logoLink to The Journal of Biophysical and Biochemical Cytology
. 1961 Feb 1;9(2):353–368. doi: 10.1083/jcb.9.2.353

ELECTRON MICROSCOPY OF PLASMA-CELL TUMORS OF THE MOUSE

I. MPC-1 and X5563 Tumors

D F Parsons 1, E B Darden Jr 1, D L Lindsley 1, Guthrie T Pratt 1
PMCID: PMC2225011  PMID: 13733008

Abstract

An electron microscope study was made of a series of transplanted MPC-1 plasma-cell tumors carried by BALB/c mice. Large numbers of particles similar in morphology to virus particles were present inside the endoplasmic reticulum of tumor plasma cells. Very few particles were seen outside the cells or in ultracentrifuged preparations of the plasma or ascites fluid. In very early tumors particles were occasionally seen free in the cytoplasm adjacent to finely granular material. In general, the distribution of these particles inside endoplasmic reticulum is similar in early and late tumors. A few transplanted X5563 tumors of C3H mice were also examined. Large numbers of particles were found in the region of the Golgi apparatus in late X5663 tumors. A newly described cytoplasmic structure of plasma cells, here called a "granular body," appears to be associated with the formation of the particles. Particles present in MPC-1 tumors are exclusively of a doughnut form, whereas some of those in the inclusions of the late X5563 tumors show a dense center. Normal plasma cells, produced by inoculation of a modified Freund adjuvant into BALB/c mice. have been compared morphologically with tumor plasma cells of both tumor lines.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ALGIRE G. H., BORDERS M. L., EVANS V. J. Studies of heterografts in diffusion chambers in mice. J Natl Cancer Inst. 1958 Jun;20(6):1187–1201. doi: 10.1093/jnci/20.6.1187. [DOI] [PubMed] [Google Scholar]
  2. BERNHARD W. Electron microscopy of tumor cells and tumor viruses; a review. Cancer Res. 1958 Jun;18(5):491–509. [PubMed] [Google Scholar]
  3. DUNN T. B. Plasma-cell neoplasms beginning in the ileocecal area in strain C3H mice. J Natl Cancer Inst. 1957 Sep;19(3):371–391. doi: 10.1093/jnci/19.3.371. [DOI] [PubMed] [Google Scholar]
  4. HOWATSON A. F., McCULLOCH E. A. Virus-like bodies in a transplantable mouse plasma cell tumour. Nature. 1958 Apr 26;181(4617):1213–1213. doi: 10.1038/1811213b0. [DOI] [PubMed] [Google Scholar]
  5. LIEBERMAN R., DOUGLAS J. O., MANTEL N. Production in mice of ascitic fluid containing antibodies induced by Staphylococcus- or Salmonella-adjuvant mixtures. J Immunol. 1960 May;84:514–529. [PubMed] [Google Scholar]
  6. PARSONS D. F., BEAUDREAU G. S., BECKER C., BONAR R. A., BEARD J. W. Cell multiplication, virus liberation, and ultrastrcuture of avain myeloblasts in tissue culture. Acta Unio Int Contra Cancrum. 1959;15:826–831. [PubMed] [Google Scholar]
  7. POTTER M., FAHEY J. L. Studies on eight transplantable plasma-cell neoplasms of mice. J Natl Cancer Inst. 1960 May;24:1153–1165. [PubMed] [Google Scholar]
  8. RASK-NIELSEN R., GORMSEN H., CLAUSEN J. A transplantable plasma-cell leukemia in mice associated with the production of beta-paraprotein. J Natl Cancer Inst. 1959 Mar;22(3):509–541. [PubMed] [Google Scholar]

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