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. 1961 Mar 1;9(3):509–518. doi: 10.1083/jcb.9.3.509

TIMING OF DNA SYNTHESIS IN THE MITOTIC CYCLE IN VITRO

Jesse E Sisken 1, Riojun Kinosita 1
PMCID: PMC2225021  PMID: 19866583

Abstract

A study was made of the timing of DNA synthesis in the mitotic cycle under conditions where the average mitotic cycle of populations of human amnion and kitten lung cells in culture was variable. Three types of experiments were performed: (a) Autoradiographs were made of incorporated tritiated thymidine in cells whose mitotic histories were recorded microcinematographically allowing the measurement of telophase + G1 along with the total length of the mitotic cycle. (b) Measurement of the G2 + prophase part of the mitotic cycle was performed under various conditions by exposing cells to tritiated thymidine and observing the increase in labeled metaphases plus anaphases as a function of time. (c) The effect of a change in pH on parts of the mitotic cycle was tested by continuously photographing a single colony of cells first at pH 7.8 and then at pH 7.1. All of our data point to the same conclusion; namely, that within a population of cells with a given generation time, the length of each of the measurable parts of the mitotic cycle has a particular distribution of values and that, when there is a change in the generation time, under our conditions only the T + G1 distribution changes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BUCHER N. L., MAZIA D. Deoxyribonucleic acid synthesis in relation to duplication of centers in dividing eggs of the sea urchin, Strongylocentrotus purpouratus. J Biophys Biochem Cytol. 1960 Jul;7:651–655. doi: 10.1083/jcb.7.4.651. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CRONKITE E. P., BOND V. P., FLIEDNER T. M., RUBINI J. R. The use of tritiated thymidine in the study of DNS synthesis and cell turnover in hemopoietic tissues. Lab Invest. 1959 Jan-Feb;8(1):263–277. [PubMed] [Google Scholar]
  3. FERNANDES M. V. The development of a human amnion strain of cells. Tex Rep Biol Med. 1958;16(1):48–58. [PubMed] [Google Scholar]
  4. FIRKET H., VERLY W. G. Autoradiographic visualization of synthesis of deoxyribonucleic acid in tissue culture with tritium-labelled thymidine. Nature. 1958 Jan 24;181(4604):274–275. doi: 10.1038/181274a0. [DOI] [PubMed] [Google Scholar]
  5. MENDELSOHN M. L., DOHAN F. C., Jr, MOORE H. A., Jr Autoradiographic analysis of cell proliferation in spontaneous breast cancer of C3H mouse. I. Typical cell cycle and timing of DNA synthesis. J Natl Cancer Inst. 1960 Sep;25:477–484. [PubMed] [Google Scholar]
  6. NAKANISHI Y. H. The development of a kitten lung cell strain and its chromosomes. Z Zellforsch Mikrosk Anat. 1960;51:138–151. doi: 10.1007/BF00343674. [DOI] [PubMed] [Google Scholar]
  7. PAINTER R. B., DREW R. M. Studies on deoxyribonucleic acid metabolism in human cancer cell cultures (HeLa). I. The temporal relationships of deoxyribonucleic acid synthesis to mitosis and turnover time. Lab Invest. 1959 Jan-Feb;8(1):278–285. [PubMed] [Google Scholar]
  8. QUASTLER H., SHERMAN F. G. Cell population kinetics in the intestinal epithelium of the mouse. Exp Cell Res. 1959 Jun;17(3):420–438. doi: 10.1016/0014-4827(59)90063-1. [DOI] [PubMed] [Google Scholar]
  9. RICHARDS B. M., WALKER P. M., DEELEY E. M. Changes in nuclear DNA in normal and ascites tumor cells. Ann N Y Acad Sci. 1956 Mar 14;63(5):831–848. doi: 10.1111/j.1749-6632.1956.tb50895.x. [DOI] [PubMed] [Google Scholar]
  10. SCHERBAUM O., RASCH G. Cell size distribution and single cell growth in Tetrahymena pyriformis GL. Acta Pathol Microbiol Scand. 1957;41(3):161–182. doi: 10.1111/j.1699-0463.1957.tb01014.x. [DOI] [PubMed] [Google Scholar]
  11. SISKEN J., OKADA T. A., ROBERTS E. Autoradiographic studies of antimitotic action of maleuric acid. Proc Soc Exp Biol Med. 1959 Jul;101(3):460–463. doi: 10.3181/00379727-101-24980. [DOI] [PubMed] [Google Scholar]
  12. SWANN M. M. The control of cell division: a review. II. Special mechanisms. Cancer Res. 1958 Nov;18(10):1118–1160. [PubMed] [Google Scholar]
  13. SWANN M. M. The control of cell division; a review. I. General mechanisms. Cancer Res. 1957 Sep;17(8):727–757. [PubMed] [Google Scholar]

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