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. 1961 Jun 1;10(2):195–209. doi: 10.1083/jcb.10.2.195

GLYCOGEN SYNTHESIS FROM URIDINE DIPHOSPHATE GLUCOSE

The Distribution of the Enzyme in Liver Cell Fractions

David J L Luck 1
PMCID: PMC2225075  PMID: 13764028

Abstract

The distribution in liver cell fractions of UDPG-glycogen transferase has been studied. In fasting animals which have been refed 6 hours before sacrifice, the distribution of the enzyme in the various cell fractions can be correlated with the glycogen content of each fraction. A purified glycogen fraction has been prepared by differential centrifugation in sucrose gradients. This glycogen fraction contains vesicular structures which resemble those seen in association with glycogen deposits in the intact liver cell. In addition, the glycogen pellet contains UDPG-glycogen transferase in high specific activity. Subfractionation of the glycogen pellet separates the majority of vesicular elements from the bulk of transferase activity and glycogen. The evidence presented suggests that the presence of UPDG-glycogen transferase in the glycogen pellet is to be attributed to its binding to glycogen rather than to its association with the structural elements found in the glycogen fraction.

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Selected References

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