Abstract
A description of the fine structure of the yolk of the unincubated hen's egg has been provided, which will serve as a basis for further studies on yolk digestion. The gross components of the yolk (that is, free-floating lipid drops, yellow and white yolk spheres together with their enclosed lipid subdroplets, and aqueous protein fluid) could be recognized by phase contrast and low power electron microscopy. The majority of the lipid drops, whether free floating or enclosed within yolk spheres, were composed of particles about 30 to 60 A in diameter. The protein component of the yolk was found to consist of round profiles about 250 A in diameter. The surfaces of the yolk spheres were of three types, and it is difficult to decide which represents the true structure although reasons are given for believing that yolk spheres are not normally enclosed by membranes identical with cell membranes.
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Selected References
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- GLAUERT A. M., GLAUERT R. H. Araldite as an embedding medium for electron microscopy. J Biophys Biochem Cytol. 1958 Mar 25;4(2):191–194. doi: 10.1083/jcb.4.2.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PALADE G. E. A small particulate component of the cytoplasm. J Biophys Biochem Cytol. 1955 Jan;1(1):59–68. doi: 10.1083/jcb.1.1.59. [DOI] [PMC free article] [PubMed] [Google Scholar]
- REVEL J. P., ITO S., FAWCETT D. W. Electron micrographs of myelin figures of phospholipide simulating intracellular membranes. J Biophys Biochem Cytol. 1958 Jul 25;4(4):495–498. doi: 10.1083/jcb.4.4.495. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROBERTSON J. D. Structural alterations in nerve fibers produced by hypotonic and hypertonic solutions. J Biophys Biochem Cytol. 1958 Jul 25;4(4):349–364. doi: 10.1083/jcb.4.4.349. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROSKIN G. I. Zheltochnye shary; k voprosu o ikh svoistvakh, stroenii i metodakh ikh issledovaniia. Izv Akad Nauk SSSR Biol. 1955 Jul-Aug;(4):112–119. [PubMed] [Google Scholar]
- STOECKENIUS W. An electron microscope study of myelin figures. J Biophys Biochem Cytol. 1959 May 25;5(3):491–500. doi: 10.1083/jcb.5.3.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WATSON M. L. Staining of tissue sections for electron microscopy with heavy metals. II. Application of solutions containing lead and barium. J Biophys Biochem Cytol. 1958 Nov 25;4(6):727–730. doi: 10.1083/jcb.4.6.727. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WATSON M. L. Staining of tissue sections for electron microscopy with heavy metals. J Biophys Biochem Cytol. 1958 Jul 25;4(4):475–478. doi: 10.1083/jcb.4.4.475. [DOI] [PMC free article] [PubMed] [Google Scholar]