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The Journal of Biophysical and Biochemical Cytology logoLink to The Journal of Biophysical and Biochemical Cytology
. 1961 Nov 1;11(2):433–440. doi: 10.1083/jcb.11.2.433

FINE STRUCTURE AND PIGMENT CONVERSION IN ISOLATED ETIOLATED PROPLASTIDS

Shimon Klein 1, A Poljakoff-Mayber 1
PMCID: PMC2225145  PMID: 14456780

Abstract

Proplastids containing a prolamellar body were isolated from leaves of etiolated bean plants. The isolation methods do not necessarily lead to destruction of their submicroscopic structure and most of the isolated proplastids show well preserved outer membranes, lamellar strands, and the prolamellar body. Morphological intactness of the proplastids varies; certain leaf fractions contain single prolamellar bodies as well as proplastids. Since pellets after centrifugation between 350 g and 1000 to 3000 g contain intact proplastids and, as was shown by quantitative experiments, the same fractions show photoconversion of protochlorophyll to chlorophyll, it is supposed that the isolated particles probably retain many of the properties which are characteristic of them in situ. Isolated proplastids may thus be a valuable tool in investigations on the development of the photosynthetic apparatus.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. KLEIN S., POLJAKOFF-MAYBER A. Isolation of proplastids from etiolated bean leaves. Exp Cell Res. 1961 Jun;24:143–145. doi: 10.1016/0014-4827(61)90255-5. [DOI] [PubMed] [Google Scholar]
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