Abstract
Methods are presented for the staining of blocks of tissue with trivalent indium so that good contrast and good specificity for nucleic acids is achieved for the electron microscope. The tissue is fixed in organic fixative, dehydrated, subjected to reduction by lithium borohydride, acetylated by acetic anhydride, stained with trivalent indium dissolved in organic solvent, and embedded. The embedding material may be either Vestopal or butyl methacrylate especially handled to eliminate the "explosion" phenomenon. Numerous new problems encountered are discussed and a brief description of the findings is included.
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Selected References
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- ALDRIDGE W. G. A new method for the differential precipitation of nucleic acids from aqueous solutions. Nature. 1960 Jul 23;187:323–324. doi: 10.1038/187323a0. [DOI] [PubMed] [Google Scholar]
- BERNSTEIN M. H. Iron as a stain for nucleic acids in electron microscopy. J Biophys Biochem Cytol. 1956 Sep 25;2(5):633–634. doi: 10.1083/jcb.2.5.633. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LAW J. H. Glycolipids. Annu Rev Biochem. 1960;29:131–150. doi: 10.1146/annurev.bi.29.070160.001023. [DOI] [PubMed] [Google Scholar]
- LEDUC E., BERNHARD W. [Attempts at ultrastructural cytochemistry. Action on chromatin]. C R Hebd Seances Acad Sci. 1960 Apr 25;250:2948–2950. [PubMed] [Google Scholar]
- LILLIE R. D. Acetylation and nitrosation of tissue amines in histochemistry. J Histochem Cytochem. 1958 Sep;6(5):352–362. doi: 10.1177/6.5.352. [DOI] [PubMed] [Google Scholar]
- WATSON M. L. Reduction of heating artifacts in thin sections examined in the electron microscope. J Biophys Biochem Cytol. 1957 Nov 25;3(6):1017–1022. doi: 10.1083/jcb.3.6.1017. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ZELANDER T., EKHOLM R. Determination of the thickness of electron microscopy sections. J Ultrastruct Res. 1960 Dec;4:413–419. doi: 10.1016/s0022-5320(60)80031-7. [DOI] [PubMed] [Google Scholar]
- van DUIJN Acrolein-Schiff, a new staining method for proteins. J Histochem Cytochem. 1961 May;9:234–241. doi: 10.1177/9.3.234. [DOI] [PubMed] [Google Scholar]