Abstract
The nerves from the walking leg of lobster released acetylcholine (ACh) even when the ends were tied off, although this release was significantly increased when the nerve endings were not tied. The resting nerves were kept in sea water containing physostigmine. In absence of physostigmine no ACh was found in the surrounding fluid. Removal of Ca from the sea water reduced the release of ACh, while increased concentrations of Ca had no significant effect. Removal of Mg++ or increased Mg++ concentrations in the presence of normal Ca++ concentrations increased the release of ACh. Increased K+ concentrations had a stimulating action on the efflux of ACh. Increased or reduced Na+ concentrations had only slight effects on the release of ACh in resting lobster nerve. During the 4 hr observation period the excised nerves were still able to synthesize ACh. The choline acetylase activity was stimulated by increased concentrations of Mg++ and K+. The effects of ions on the release of ACh are similar to those reported at the junction.
Full Text
The Full Text of this article is available as a PDF (791.0 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- ARMETT C. J., RITCHIE J. M. The action of acetylcholine on conduction in mammalian non-myelinated fibres and its prevention by an anticholinesterase. J Physiol. 1960 Jun;152:141–158. doi: 10.1113/jphysiol.1960.sp006476. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brown G. L., Feldberg W. The action of potassium on the superior cervical ganglion of the cat. J Physiol. 1936 Mar 9;86(3):290–305. doi: 10.1113/jphysiol.1936.sp003364. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chang H. C., Gaddum J. H. Choline esters in tissue extracts. J Physiol. 1933 Oct 6;79(3):255–285. doi: 10.1113/jphysiol.1933.sp003049. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DEL CASTILLO J., ENGBAEK L. The nature of the neuromuscular block produced by magnesium. J Physiol. 1954 May 28;124(2):370–384. doi: 10.1113/jphysiol.1954.sp005114. [DOI] [PMC free article] [PubMed] [Google Scholar]
- DETTBARN W. D. ACTION OF ACETYLCHOLINE AND CURARE ON LOBSTER AXONS. Life Sci. 1963 Dec;12:910–916. doi: 10.1016/0024-3205(63)90059-6. [DOI] [PubMed] [Google Scholar]
- DETTBARN W. D., DAVIS F. A. Effect of acetylcholine on the electrical activity of somatic nerves of the lobster. Science. 1962 May 25;136(3517):716–717. doi: 10.1126/science.136.3517.716. [DOI] [PubMed] [Google Scholar]
- DETTBARN W. D., DAVIS F. A. Effects of acetylcholine on axonal conduction of lobster nerve. Biochim Biophys Acta. 1963 May 21;66:397–405. doi: 10.1016/0006-3002(63)91208-3. [DOI] [PubMed] [Google Scholar]
- Dale H. H., Feldberg W., Vogt M. Release of acetylcholine at voluntary motor nerve endings. J Physiol. 1936 May 4;86(4):353–380. doi: 10.1113/jphysiol.1936.sp003371. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ECCLES J. C. The mechanism of synaptic transmission. Ergeb Physiol. 1961;51:299–430. [PubMed] [Google Scholar]
- FLOREY E., BIEDERMAN M. A. Studies on the distribution of factor I and acetylcholine in crustacean peripheral nerve. J Gen Physiol. 1960 Jan;43:509–522. doi: 10.1085/jgp.43.3.509. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Feldberg W., Hebb C. The effects of magnesium ions and of creatine phosphate on the synthesis of acetylcholine. J Physiol. 1947 Mar 15;106(1):8–17. [PMC free article] [PubMed] [Google Scholar]
- Feldberg W. Synthesis of acetylcholine by tissue of the central nervous system. J Physiol. 1945 Mar 28;103(4):367–402. doi: 10.1113/jphysiol.1945.sp004085. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GERHARDS K. P., ROETTCHER M., STRAUB R. W. WIRKUNGEN VON CA UND MG AUF FREISETZUNG UND SYNTHESE VON ACETYLCHOLIN AM RUHIGGESTELLTEN DARM. Pflugers Arch Gesamte Physiol Menschen Tiere. 1964 Apr 29;279:251–264. [PubMed] [Google Scholar]
- HUBBARD J. I. The effect of calcium and magnesium on the spontaneous release of transmitter from mammalian motor nerve endings. J Physiol. 1961 Dec;159:507–517. doi: 10.1113/jphysiol.1961.sp006824. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Harvey A. M., Macintosh F. C. Calcium and synaptic transmission in a sympathetic ganglion. J Physiol. 1940 Jan 15;97(3):408–416. doi: 10.1113/jphysiol.1940.sp003818. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KATZ B., MILEDI R. A STUDY OF SPONTANEOUS MINIATURE POTENTIALS IN SPINAL MOTONEURONES. J Physiol. 1963 Sep;168:389–422. doi: 10.1113/jphysiol.1963.sp007199. [DOI] [PMC free article] [PubMed] [Google Scholar]
- LILEY A. W. The effects of presynaptic polarization on the spontaneous activity at the mammalian neuromuscular junction. J Physiol. 1956 Nov 28;134(2):427–443. doi: 10.1113/jphysiol.1956.sp005655. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Macintosh F. C. The distribution of acetylcholine in the peripheral and the central nervous system. J Physiol. 1941 Jun 30;99(4):436–442. doi: 10.1113/jphysiol.1941.sp003913. [DOI] [PMC free article] [PubMed] [Google Scholar]
- ROSENBERG P., HOSKIN F. C. Demonstration of increased permeability as a factor in the effect of acetylcholine on the electrical activty of venom-treated axons. J Gen Physiol. 1963 May;46:1065–1073. doi: 10.1085/jgp.46.5.1065. [DOI] [PMC free article] [PubMed] [Google Scholar]