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. 1967 Jul 1;50(6):1485–1497. doi: 10.1085/jgp.50.6.1485

The Effect of Low Ionic Strength Extracellular Solutions on the Resting Potential in Skeletal Muscle Fibers

David Holtzman 1
PMCID: PMC2225731  PMID: 6034754

Abstract

Intracellular measurements of the resting potential were made in fibers of the frog sartorius muscle in solutions of varying salt composition and concentration to determine the effects of low ionic strength extracellular solutions on the resting potential. Changes in the glass microelectrode tip potential in low ionic strength solutions were minimized by adding ThCl4 to the extracellular solution. These experimental conditions allowed measurement of the relationship of the resting potential to the concentration of the salt in the extracellular solution by replacing it with the nonionic substance, sucrose. Substitution of sucrose for the extracellular NaCl produced a stable depolarization which was logarithmically related to the NaCl concentration. Substitution of sucrose for choline Cl, instead of NaCl, produced the same degree of depolarization. When Na salts of anions less permeable than chloride (Br, I, NO3) were used, the resting potentials in 116 mM solutions were close to those with chloride (±3mv). The depolarizations produced in low ionic strength solutions of these salts were significantly less than those with chloride.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADRIAN R. H. Internal chloride concentration and chloride efflux of frog muscle. J Physiol. 1961 May;156:623–632. doi: 10.1113/jphysiol.1961.sp006698. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ADRIAN R. H. The effect of internal and external potassium concentration on the membrane potential of frog muscle. J Physiol. 1956 Sep 27;133(3):631–658. doi: 10.1113/jphysiol.1956.sp005615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Agin D., Holtzman D. Glass microelectrodes: the origin and elimination of tip potentials. Nature. 1966 Sep 10;211(5054):1194–1195. doi: 10.1038/2111194a0. [DOI] [PubMed] [Google Scholar]
  4. GIEBISCH G., KRAUPP O., PILLAT B., STORMANN H. Der Ersatz von extracellulärem Natriumchlorid durch Natriumsulfat bzw. Saccharose und seine Wirkung auf die isoliert durchströmte Saugetiermuskulatur. Pflugers Arch. 1957;265(3):220–236. doi: 10.1007/BF00595649. [DOI] [PubMed] [Google Scholar]
  5. HARRIS E. J. THE CHLORIDE PERMEABILITY OF FROG SARTORIUS. J Physiol. 1965 Jan;176:123–135. doi: 10.1113/jphysiol.1965.sp007539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. HODGKIN A. L., HOROWICZ P. The effect of nitrate and other anions on the mechanical response of single muscle fibres. J Physiol. 1960 Sep;153:404–412. doi: 10.1113/jphysiol.1960.sp006542. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. HOROWICZ P. THE EFFECTS OF ANIONS ON EXCITABLE CELLS. Pharmacol Rev. 1964 Jun;16:193–221. [PubMed] [Google Scholar]
  8. MULLINS L. J., NODA K. THE INFLUENCE OF SODIUM-FREE SOLUTIONS ON THE MEMBRANE POTENTIAL OF FROG MUSCLE FIBERS. J Gen Physiol. 1963 Sep;47:117–132. doi: 10.1085/jgp.47.1.117. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. SANDOW A. Contracture responses of skeletal muscle. Am J Phys Med. 1955 Feb;34(1):145–160. [PubMed] [Google Scholar]
  10. SZAIMI T., TOMITA T. ELECTRICAL PROPERTIES OF THE FROG SKELETAL MUSCLE MEMBRANE IN CL-FREE SULPHATE-, FERROCYANIDE-, AND GLUTAMATE-RINGER'S SOLUTIONS. Jpn J Physiol. 1963 Dec 15;13:641–656. doi: 10.2170/jjphysiol.13.641. [DOI] [PubMed] [Google Scholar]
  11. TSOFINA L. M., LIBERMAN E. A. ANIONY I RABOTA VOZBUDIMYKH TKANE I. Biofizika. 1964;9:242–254. [PubMed] [Google Scholar]

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