Abstract
Continuous culture of coli 12λ, P22, 600-434, 600-21, and 600-299 in the presence of triethylenemelamine (TEM) results in the appearance of a new virulent virus which attacks the parent culture. N-Methyl-N'-nitro-N-nitrosoguanidine (MNNG) is effective with 600-21 and ultraviolet light with 12λ and 600-21. The cultures which produce the virulent virus continue to do so indefinitely in the absence of the mutagen, but are not lysogenic for the virus. Most of the cells in such cultures are resistant to the virus and do not produce any, but there are a few mutant cells sensitive to the virus and the virus multiplies by infection of these sensitive mutants.
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Selected References
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- Bertani L. E. Limited multiplication of phages superinfecting lysogenic bacteria and its implication for the mechanism of immunity. Virology. 1965 Dec;27(4):496–511. doi: 10.1016/0042-6822(65)90175-3. [DOI] [PubMed] [Google Scholar]
- DURAN-REYNALS F. Studies on the combined effects of fowl pox virus and methylcholanthrene in chickens. Ann N Y Acad Sci. 1952 Jul 10;54(6):977–991. doi: 10.1111/j.1749-6632.1952.tb39973.x. [DOI] [PubMed] [Google Scholar]
- GROSS L. Attempt to recover filterable agent from x-ray induced leukemia. Acta Haematol. 1958 Jun;19(6):353–361. doi: 10.1159/000205455. [DOI] [PubMed] [Google Scholar]
- JACOB F., WOLLMAN E. L. Etude génétique d'un bactériophage tempéré d'Escherichia coli. l. Le système genétique du bactériophage. Ann Inst Pasteur (Paris) 1954 Dec;87(6):653–673. [PubMed] [Google Scholar]
- KAISER A. D., JACOB F. Recombination between related temperate bacteriophages and the genetic control of immunity and prophage localization. Virology. 1957 Dec;4(3):509–521. doi: 10.1016/0042-6822(57)90083-1. [DOI] [PubMed] [Google Scholar]
- Lederberg E M, Lederberg J. Genetic Studies of Lysogenicity in Escherichia Coli. Genetics. 1953 Jan;38(1):51–64. doi: 10.1093/genetics/38.1.51. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Northrop J. H. Increase in the proportion of virus-producing cells and of several mutants in K12-lambda cultures exposed to ultraviolet light, triethylene melamine, or hydrogen peroxide. Enzymologia. 1966 Feb 28;30(2):131–134. [PubMed] [Google Scholar]
- Northrop J. H. Production of a new bacterial virus by prolonged growth of lysogenic E. coli cultures in the presence of triethylene melamine. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1632–1635. doi: 10.1073/pnas.54.6.1632. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Singer B., Fraenkel-Conrat H. Chemical modification of viral RNA. VI. The action of N-methyl-N'-nitro-N-nitrosoguanidine. Proc Natl Acad Sci U S A. 1967 Jul;58(1):234–239. doi: 10.1073/pnas.58.1.234. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wheeler G. P. Symposium on immunosuppressive drugs. Some biochemical effects of alkylating agents. Fed Proc. 1967 May-Jun;26(3):885–892. [PubMed] [Google Scholar]
- YAMAFUJI K. METABOLIC VIROGENS HAVING MUTAGENIC ACTION AND CHROMOSOMAL PREVIRUSES. Enzymologia. 1964 Sep 30;27:217–274. [PubMed] [Google Scholar]