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. 1968 Aug 1;52(2):363–386. doi: 10.1085/jgp.52.2.363

Effects of Some Organic Cations on Generator Potential of Crayfish Stretch Receptor

Shosaku Obara 1
PMCID: PMC2225805  PMID: 5672006

Abstract

The generator potential of both slowly and rapidly adapting crayfish stretch receptor cells can still be elicited by mechanical stimuli when all the Na of the bathing medium is replaced by various organic cations. In the presence of tris(hydroxymethyl)aminomethane (Tris), the generator potential is particularly large, about 30–50 % of that in the control saline, while spike electrogenesis of the cell is abolished. Persistence of the generator response is not due to retention of Na by a diffusion barrier, and ionic contributions to the electrogenesis by Ca and Cl can also be excluded. Thus, whereas the electrogenesis of the generator membrane must be due to an increased permeability to monovalent cations, the active receptor membrane appears to be less selective for different monovalent cations than is the receptor component of some other cells, or the conductile component of the stretch receptor neuron.

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Selected References

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  1. BATES R. G. Amine buffers for pH control. Ann N Y Acad Sci. 1961 Jun 17;92:341–356. doi: 10.1111/j.1749-6632.1961.tb44985.x. [DOI] [PubMed] [Google Scholar]
  2. BURKE W., GINSBORG B. L. The action of the neuromuscular transmitter on the slow fibre membrane. J Physiol. 1956 Jun 28;132(3):599–610. doi: 10.1113/jphysiol.1956.sp005552. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. DIAMOND J., GRAY J. A., INMAN D. R. The relation between receptor potentials and the concentration of sodium ions. J Physiol. 1958 Jul 14;142(2):382–394. doi: 10.1113/jphysiol.1958.sp006024. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. EDWARDS C., HAGIWARA S. Potassium ions and the inhibitory process in the crayfish stretch receptor. J Gen Physiol. 1959 Nov;43:315–321. doi: 10.1085/jgp.43.2.315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. EDWARDS C., TERZUOLO C. A., WASHIZU H. THE EFFECT OF CHANGES OF THE IONIC ENVIRONMENT UPON AN ISOLATED CRUSTACEAN SENSORY NEURON. J Neurophysiol. 1963 Nov;26:948–957. doi: 10.1152/jn.1963.26.6.948. [DOI] [PubMed] [Google Scholar]
  6. EYZAGUIRRE C., KUFFLER S. W. Further study of soma, dendrite, and axon excitation in single neurons. J Gen Physiol. 1955 Sep 20;39(1):121–153. doi: 10.1085/jgp.39.1.121. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. FLOREY E., FLOREY E. Microanatomy of the abdominal stretch receptors of the crayfish (Astacus fluviatilis L.). J Gen Physiol. 1955 Sep 20;39(1):69–85. doi: 10.1085/jgp.39.1.69. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. FURUKAWA T., FURUKAWA A. Effects of methyl- and ethyl-derivatives of NH4 on the neuromuscular junction. Jpn J Physiol. 1959 Jun 25;9(2):130–142. doi: 10.2170/jjphysiol.9.130. [DOI] [PubMed] [Google Scholar]
  9. FURUKAWA T., FURUKAWA A., TAKAGI T. Fibrillation of muscle fibers produced by ammonium ions and its relation to the spontaneous activity at the neuromuscular junction. Jpn J Physiol. 1957 Sep 30;7(3):252–263. doi: 10.2170/jjphysiol.7.252. [DOI] [PubMed] [Google Scholar]
  10. FURUKAWA T., HANAWA I. Effects of some common cations on electroretinogram of the toad. Jpn J Physiol. 1955 Dec 15;5(4):289–300. doi: 10.2170/jjphysiol.5.289. [DOI] [PubMed] [Google Scholar]
  11. HAGIWARA S., KUSANO K., SAITO S. Membrane changes in crayfish stretch receptor neutron during synaptic inhibition and under action of gamma-aminobutyric acid. J Neurophysiol. 1960 Sep;23:505–515. doi: 10.1152/jn.1960.23.5.505. [DOI] [PubMed] [Google Scholar]
  12. KATZ B. Depolarization of sensory terminals and the initiation of impulses in the muscle spindle. J Physiol. 1950 Oct 16;111(3-4):261–282. doi: 10.1113/jphysiol.1950.sp004479. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. KOKETSU K., NISHI S. Restoration of neuromuscular transmission in sodium-free hydrazinium solution. J Physiol. 1959 Sep 2;147:239–252. doi: 10.1113/jphysiol.1959.sp006239. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. KUFFLER S. W., EYZAGUIRRE C. Synaptic inhibition in an isolated nerve cell. J Gen Physiol. 1955 Sep 20;39(1):155–184. doi: 10.1085/jgp.39.1.155. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. LOEWENSTEIN W. R., TERZUOLO C. A., WASHIZU Y. SEPARATION OF TRANSDUCER AND IMPULSE-GENERATING PROCESSES IN SENSORY RECEPTORS. Science. 1963 Nov 29;142(3596):1180–1181. doi: 10.1126/science.142.3596.1180. [DOI] [PubMed] [Google Scholar]
  16. LUTTGAU H. C., NIEDERGERKE R. The antagonism between Ca and Na ions on the frog's heart. J Physiol. 1958 Oct 31;143(3):486–505. doi: 10.1113/jphysiol.1958.sp006073. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Millecchia R., Bradbury J., Mauro A. Simple photoreceptors in Limulus polyphemus. Science. 1966 Dec 2;154(3753):1199–1201. doi: 10.1126/science.154.3753.1199. [DOI] [PubMed] [Google Scholar]
  18. NAKAJIMA S. ADAPTATION IN STRETCH RECEPTOR NEURONS OF CRAYFISH. Science. 1964 Nov 27;146(3648):1168–1170. doi: 10.1126/science.146.3648.1168. [DOI] [PubMed] [Google Scholar]
  19. NASTUK W. L. Some ionic factors that influence the action of acetylcholine at the muscle end-plate membrane. Ann N Y Acad Sci. 1959 Aug 28;81:317–327. doi: 10.1111/j.1749-6632.1959.tb49316.x. [DOI] [PubMed] [Google Scholar]
  20. Nakajima S., Takahashi K. Post-tetanic hyperpolarization and electrogenic Na pump in stretch receptor neurone of crayfish. J Physiol. 1966 Nov;187(1):105–127. doi: 10.1113/jphysiol.1966.sp008078. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. OMACHI A., MACEY R. I., WALDECK J. G. Permeability of cell membranes to amine buffers and their effects on electrolyte transport. Ann N Y Acad Sci. 1961 Jun 17;92:478–485. doi: 10.1111/j.1749-6632.1961.tb44997.x. [DOI] [PubMed] [Google Scholar]
  22. OTTOSON D. THE EFFECT OF SODIUM DEFICIENCY ON THE RESPONSE OF THE ISOLATED MUSCLE SPINDLE. J Physiol. 1964 May;171:109–118. doi: 10.1113/jphysiol.1964.sp007365. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Obara S., Grundfest H. Effects of lithium on different membrane components of crayfish stretch receptor neurons. J Gen Physiol. 1968 May;51(5):635–654. doi: 10.1085/jgp.51.5.635. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Ozeki M., Grundfest H. Crayfish muscle fiber: ionic requirements for depolarizing synaptic electrogenesis. Science. 1967 Jan 27;155(3761):478–481. doi: 10.1126/science.155.3761.478. [DOI] [PubMed] [Google Scholar]
  25. TAKEUCHI N. Effects of calcium on the conductance change of the end-plate membrane during the action of transmitter. J Physiol. 1963 Jun;167:141–155. doi: 10.1113/jphysiol.1963.sp007137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. TERZUOLO C. A., WASHIZU Y. Relation between stimulus strength, generator potential and impulse frequency in stretch receptor of Crustacea. J Neurophysiol. 1962 Jan;25:56–66. doi: 10.1152/jn.1962.25.1.56. [DOI] [PubMed] [Google Scholar]
  27. Takeda K. Permeability changes associated with the action potential in procaine-treated crayfish abdominal muscle fibers. J Gen Physiol. 1967 Mar;50(4):1049–1074. doi: 10.1085/jgp.50.4.1049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. WERMAN R., GRUNDFEST H. Graded and all-or-none electrogenesis in arthropod muscle. II. The effects of alkali-earth and onium ions on lobster muscle fibers. J Gen Physiol. 1961 May;44:997–1027. doi: 10.1085/jgp.44.5.997. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. WERMAN R., McCANN F. V., GRUNDFEST H. Graded and all-or-none electrogenesis in arthropod muscle. I. The effects of alkali-earth cations on the neuromuscular system of Romalea microptera. J Gen Physiol. 1961 May;44:979–995. doi: 10.1085/jgp.44.5.979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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