Abstract
The relation between oxygen consumption and motility of Ciona spermatozoa has been measured by using pH stats to measure the acid production of spermatozoa swimming in dilute suspensions where their motility can be analyzed accurately, and calibrating the acid production by measuring it simultaneously with measurements of oxygen consumption, using more concentrated sperm suspensions. When the motility of the spermatozoa is inhibited by thiourea or by increased viscosity, their oxygen consumption decreases in proportion to the decrease in beat frequency. 80–85 % of their oxygen consumption appears to be tightly coupled to motility. The amount of movement-coupled oxidative metabolism per beat remains nearly constant, even when there are significant changes in the energy required per beat for movement against the viscous resistance of the medium. This implies that under these conditions, where the radius of curvature of flagellar bending remains constant, the amount of ATP used is determined by a stoichiometric relation to bending rather than by the energy requirement. The movement-coupled oxidative metabolism appears to be sufficient to generate approximately two molecules of ATP per beat for each molecule of the flagellar ATPase, dynein.
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Selected References
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- Brokaw C. J. Adenosine triphosphate usage by flagella. Science. 1967 Apr 7;156(3771):76–78. doi: 10.1126/science.156.3771.76. [DOI] [PubMed] [Google Scholar]
- Brokaw C. J., Benedict B. Mechanochemical coupling in flagella. I. Movement-dependent dephosphorylation of ATP by glycerinated spermatozoa. Arch Biochem Biophys. 1968 Jun;125(3):770–778. doi: 10.1016/0003-9861(68)90513-4. [DOI] [PubMed] [Google Scholar]
- Brokaw C. J. Effects of increased viscosity on the movements of some invertebrate spermatozoa. J Exp Biol. 1966 Aug;45(1):113–139. doi: 10.1242/jeb.45.1.113. [DOI] [PubMed] [Google Scholar]
- Brokaw C. J. Mechanisms of sperm movement. Symp Soc Exp Biol. 1968;22:101–116. [PubMed] [Google Scholar]
- Brokaw C. J. Non-sinusoidal bending waves of sperm flagella. J Exp Biol. 1965 Aug;43(1):155–169. doi: 10.1242/jeb.43.1.155. [DOI] [PubMed] [Google Scholar]
- CARLSON F. D., HARDY D. J., WILKIE D. R. Total energy production and phosphocreatine hydrolysis in the isotonic twitch. J Gen Physiol. 1963 May;46:851–882. doi: 10.1085/jgp.46.5.851. [DOI] [PMC free article] [PubMed] [Google Scholar]
- NEVO A. C., CAPLAN S. R., SCHINDLER H. DURATION OF MOTILITY AND GLYCOLYSIS OF FOWL SPERMATOZOA IN VITRO UNDER ANAEROBIC CONDITIONS, CONSTANT PH AND CONSTANT GLUCOSE CONCENTRATION. J Reprod Fertil. 1963 Dec;6:361–370. doi: 10.1530/jrf.0.0060361. [DOI] [PubMed] [Google Scholar]
- RIKMENSPOEL R. THE INHIBITION BY AMYTAL OF RESPIRATION AND MOTILITY OF BULL SPERMATOZOA. Exp Cell Res. 1965 Feb;37:312–326. doi: 10.1016/0014-4827(65)90180-1. [DOI] [PubMed] [Google Scholar]