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. 1970 Jul 1;56(1):83–99. doi: 10.1085/jgp.56.1.83

Direct Measurement of Uptake of Sodium at the Outer Surface of the Frog Skin

Thomas U L Biber 1, Peter F Curran 1
PMCID: PMC2225880  PMID: 5514162

Abstract

A combination of the methods described by Schultz et al. (6) and by Ussing and Zerahn (9) was used to measure directly the unidirectional uptake of sodium from the outside solution into the frog skin, under short-circuit conditions. The sodium uptake was determined at six sodium concentrations ranging from 3.4 to 114 mM. NaCl was replaced by choline chloride in the solutions bathing both sides of the skin. Sodium uptake is not a linear function of sodium concentration but appears to be composed of two components, a saturating one and one that varies linearly with concentration. The sodium uptake is inhibited by the addition of lithium to the outside solution. The effect appears to be primarily on the saturating component and has the characteristics of competitive inhibition. In addition, lithium uptake by the skin is inhibited by sodium. The effects of lithium cannot be ascribed to changes in electrical potential difference. Measurements with microelectrodes indicate that under short-circuit condition there is no change in the intracellular potential when lithium chloride is added to the outside solution.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDERSEN B., ZERAHN K. METHOD FOR NON-DESTRUCTIVE DETERMINATION OF THE SODIUM TRANSPORT POOL IN FROG SKIN WITH RADIOSODIUM. Acta Physiol Scand. 1963 Dec;59:319–329. doi: 10.1111/j.1748-1716.1963.tb02747.x. [DOI] [PubMed] [Google Scholar]
  2. Biber T. U., Chez R. A., Curran P. F. Na transport across frog skin at low external Na concentrations. J Gen Physiol. 1966 Jul;49(6):1161–1176. doi: 10.1085/jgp.0491161. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. CEREIJIDO M., CURRAN P. F. INTRACELLULAR ELECTRICAL POTENTIALS IN FROG SKIN. J Gen Physiol. 1965 Mar;48:543–557. doi: 10.1085/jgp.48.4.543. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CEREIJIDO M., HERRERA F. C., FLANIGAN W. J., CURRAN P. F. THE INFLUENCE OF NA CONCENTRATION ON NA TRANSPORT ACROSS FROG SKIN. J Gen Physiol. 1964 May;47:879–893. doi: 10.1085/jgp.47.5.879. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cereijido M., Rotunno C. A. Transport and distribution of sodium across frog skin. J Physiol. 1967 Jun;190(3):481–497. doi: 10.1113/jphysiol.1967.sp008223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Curran P. F. Coupling between transport processes in intestine. Physiologist. 1968 Feb;11(1):3–23. [PubMed] [Google Scholar]
  7. DOWD J. E., RIGGS D. S. A COMPARISON OF ESTIMATES OF MICHAELIS-MENTEN KINETIC CONSTANTS FROM VARIOUS LINEAR TRANSFORMATIONS. J Biol Chem. 1965 Feb;240:863–869. [PubMed] [Google Scholar]
  8. FRAZIER H. S., DEMPSEY E. F., LEAF A. Movement of sodium across the mucosal surface of the isolated toad bladder and its modification by vasopressin. J Gen Physiol. 1962 Jan;45:529–543. doi: 10.1085/jgp.45.3.529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Goldner A. M., Schultz S. G., Curran P. F. Sodium and sugar fluxes across the mucosal border of rabbit ileum. J Gen Physiol. 1969 Mar;53(3):362–383. doi: 10.1085/jgp.53.3.362. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. HANSEN H. H., ZERAHN K. CONCENTRATION OF LITHIUM, SODIUM AND POTASSIUM IN EPITHELIAL CELLS OF THE ISOLATED FROG SKIN DURING ACTIVE TRANSPORT OF LITHIUM. Acta Physiol Scand. 1964 Jan-Feb;60:189–196. doi: 10.1111/j.1748-1716.1964.tb02882.x. [DOI] [PubMed] [Google Scholar]
  11. KIDDER G. W., 3rd, CEREIJIDO M., CURRAN P. F. TRANSIENT CHANGES IN ELECTRICAL POTENTIAL DIFFERENCES ACROSS FROG SKIN. Am J Physiol. 1964 Oct;207:935–940. doi: 10.1152/ajplegacy.1964.207.4.935. [DOI] [PubMed] [Google Scholar]
  12. KOEFOED-JOHNSEN V., USSING H. H. The nature of the frog skin potential. Acta Physiol Scand. 1958 Jun 2;42(3-4):298–308. doi: 10.1111/j.1748-1716.1958.tb01563.x. [DOI] [PubMed] [Google Scholar]
  13. Routunno C. A., Pouchan M. I., Cereijido M. Location of the mechanism of active transport of sodium across the frog skin. Nature. 1966 May 7;210(5036):597–599. doi: 10.1038/210597a0. [DOI] [PubMed] [Google Scholar]
  14. Schultz S. G., Curran P. F., Chez R. A., Fuisz R. E. Alanine and sodium fluxes across mucosal border of rabbit ileum. J Gen Physiol. 1967 May;50(5):1241–1260. doi: 10.1085/jgp.50.5.1241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. USSING H. H., WINDHAGER E. E. NATURE OF SHUNT PATH AND ACTIVE SODIUM TRANSPORT PATH THROUGH FROG SKIN EPITHELIUM. Acta Physiol Scand. 1964 Aug;61:484–504. [PubMed] [Google Scholar]
  16. USSING H. H., ZERAHN K. Active transport of sodium as the source of electric current in the short-circuited isolated frog skin. Acta Physiol Scand. 1951 Aug 25;23(2-3):110–127. doi: 10.1111/j.1748-1716.1951.tb00800.x. [DOI] [PubMed] [Google Scholar]
  17. Voûte C. L., Ussing H. H. Some morphological aspects of active sodium transport. The epithelium of the frog skin. J Cell Biol. 1968 Mar;36(3):625–638. doi: 10.1083/jcb.36.3.625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. WHITTEMBURY G. ELECTRICAL POTENTIAL PROFILE OF THE TOAD SKIN EPITHELIUM. J Gen Physiol. 1964 Mar;47:795–808. doi: 10.1085/jgp.47.4.795. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. ZERAHN K. Studies on the active transport of lithium in the isolated frog skin. Acta Physiol Scand. 1955 Aug 19;33(4):347–358. doi: 10.1111/j.1748-1716.1955.tb01214.x. [DOI] [PubMed] [Google Scholar]
  20. Zerahn K. Nature and localization of the sodium pool during active transport in the isolated frog skin. Acta Physiol Scand. 1969 Nov;77(3):272–281. doi: 10.1111/j.1748-1716.1969.tb04572.x. [DOI] [PubMed] [Google Scholar]

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