Abstract
Nystatin and amphotericin B increase the permeability of thin (<100 A) lipid membranes to ions, water, and nonelectrolytes. Water and nonelectrolyte permeability increase linearly with membrane conductance (i.e., ion permeability). In the unmodified membrane, the osmotic permeability coefficient, Pf, is equal to the tagged water permeability coefficient, (Pd)w; in the nystatin- or amphotericin B-treated membrane, Pf/(Pd)w ≈ 3. The unmodified membrane is virtually impermeable to small hydrophilic solutes, such as urea, ethylene glycol, and glycerol; the nystatin- or amphotericin B-treated membrane displays a graded permeability to these solutes on the basis of size. This graded permeability is manifest both in the tracer permeabilities, Pd, and in the reflection coefficients, σ (Table I). The "cutoff" in permeability occurs with molecules about the size of glucose (Stokes-Einstein radius ≊ 4 A). We conclude that nystatin and amphotericin B create aqueous pores in thin lipid membranes; the effective radius of these pores is approximately 4 A. There is a marked similarity between the permeability of a nystatin- or amphotericin B-treated membrane to water and small hydrophilic solutes and the permeability of the human red cell membrane to these same molecules.
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- ALBERT A. Design of chelating agents for selected biological activity. Fed Proc. 1961 Sep;20(3):137–147. [PubMed] [Google Scholar]
- Andreoli T. E., Dennis V. W., Weigl A. M. The effect of amphotericin B on the water and nonelectrolyte permeability of thin lipid membranes. J Gen Physiol. 1969 Feb;53(2):133–156. doi: 10.1085/jgp.53.2.133. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bean R. C., Shepherd W. C., Chan H. Permeability of lipid bilayer membranes to organic solutes. J Gen Physiol. 1968 Sep;52(3):495–508. doi: 10.1085/jgp.52.3.495. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cass A., Finkelstein A., Krespi V. The ion permeability induced in thin lipid membranes by the polyene antibiotics nystatin and amphotericin B. J Gen Physiol. 1970 Jul;56(1):100–124. doi: 10.1085/jgp.56.1.100. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cass A., Finkelstein A. Water permeability of thin lipid membranes. J Gen Physiol. 1967 Jul;50(6):1765–1784. doi: 10.1085/jgp.50.6.1765. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Everitt C. T., Redwood W. R., Haydon D. A. Problem of boundary layers in the exchange diffusion of water across bimolecular lipid membranes. J Theor Biol. 1969 Jan;22(1):20–32. doi: 10.1016/0022-5193(69)90077-0. [DOI] [PubMed] [Google Scholar]
- Finkelstein A., Cass A. Effect of cholesterol on the water permeability of thin lipid membranes. Nature. 1967 Nov 18;216(5116):717–718. doi: 10.1038/216717a0. [DOI] [PubMed] [Google Scholar]
- GOLDSTEIN D. A., SOLOMON A. K. Determination of equivalent pore radius for human red cells by osmotic pressure measurement. J Gen Physiol. 1960 Sep;44:1–17. doi: 10.1085/jgp.44.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KEDEM O., KATCHALSKY A. Thermodynamic analysis of the permeability of biological membranes to non-electrolytes. Biochim Biophys Acta. 1958 Feb;27(2):229–246. doi: 10.1016/0006-3002(58)90330-5. [DOI] [PubMed] [Google Scholar]
- Lippe C. Effects of Amphotericin B on thiourea permeability of phospholipid and cholesterol bilayer membranes. J Mol Biol. 1968 Aug 14;35(3):635–637. doi: 10.1016/s0022-2836(68)80019-1. [DOI] [PubMed] [Google Scholar]
- PAGANELLI C. V., SOLOMON A. K. The rate of exchange of tritiated water across the human red cell membrane. J Gen Physiol. 1957 Nov 20;41(2):259–277. doi: 10.1085/jgp.41.2.259. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RENKIN E. M. Filtration, diffusion, and molecular sieving through porous cellulose membranes. J Gen Physiol. 1954 Nov 20;38(2):225–243. [PMC free article] [PubMed] [Google Scholar]