Skip to main content
The Journal of General Physiology logoLink to The Journal of General Physiology
. 1969 Jul 1;54(1):134–143. doi: 10.1085/jgp.54.1.134

Effects of Polypeptide and Protein Hormones on Lipid Monolayers

I. Effect of insulin and parathyroid hormone on monomolecular films of monooctadecyl phosphate and stearic acid

Marian S Kafka 1, Charles Y C Pak 1
PMCID: PMC2225915  PMID: 5792362

Abstract

Insulin in low concentrations inhibits the uptake of Ca++ by the monooctadecyl (stearyl) phosphate monolayer (at air-water interface) and facilitates the release of Ca++ adsorbed to the monolayer. These effects of insulin are more pronounced at higher insulin concentrations. Evidence is presented that a relatively intact insulin molecule competes with Ca++ for the free phosphate group of the monolayer. Albumin has a slight inhibitory action on calcium uptake and parathyroid hormone has no observable action on calcium uptake or release.

Full Text

The Full Text of this article is available as a PDF (619.9 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arnold J. D., Pak C. Y. Interaction of soluble proteins with protein monolayers. J Am Oil Chem Soc. 1968 Mar;45(3):128–138. doi: 10.1007/BF02915336. [DOI] [PubMed] [Google Scholar]
  2. Blaustein M. P., Goldman D. E. Competitive action of calcium and procaine on lobster axon. A study of the mechanism of action of certain local anesthetics. J Gen Physiol. 1966 May;49(5):1043–1063. doi: 10.1085/jgp.49.5.1043. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blecher M. Phospholipase C and mechanisms of action of insulin and cortisol on glucose entry into free adipose cells. Biochem Biophys Res Commun. 1965 Nov 8;21(3):202–209. doi: 10.1016/0006-291x(65)90272-x. [DOI] [PubMed] [Google Scholar]
  4. Bozler E. Osmotic properties of amphibian muscles. J Gen Physiol. 1965 Sep;49(1):37–45. doi: 10.1085/jgp.49.1.37. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. CROFFORD O. B., RENOLD A. E. GLUCOSE UPTAKE BY INCUBATED RAT EPIDIDYMAL ADIPOSE TISSUE. CHARACTERISTICS OF THE GLUCOSE TRANSPORT SYSTEM AND ACTION OF INSULIN. J Biol Chem. 1965 Aug;240:3237–3244. [PubMed] [Google Scholar]
  6. MORGAN H. E., HENDERSON M. J., REGEN D. M., PARK C. R. Regulation of glucose uptake in heart muscle from normal and alloxan-diabetic rats: the effects of insulin, growth hormone, cortisone, and anoxia. Ann N Y Acad Sci. 1959 Sep 25;82:387–402. doi: 10.1111/j.1749-6632.1959.tb44920.x. [DOI] [PubMed] [Google Scholar]
  7. Manery J. F. Effects of Ca ions on membranes. Fed Proc. 1966 Nov-Dec;25(6):1804–1810. [PubMed] [Google Scholar]
  8. Morrill G. A., Robbins E. The role of calcium in the regulation of the steady-state levels of sodium and potassium in the HeLa cell. J Gen Physiol. 1967 Mar;50(4):781–792. doi: 10.1085/jgp.50.4.781. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. ROSS E. J. The permeability hypothesis of the action of insulin. Medicine (Baltimore) 1956 Dec;35(4):355–388. doi: 10.1097/00005792-195612000-00002. [DOI] [PubMed] [Google Scholar]
  10. Rodbell M. Metabolism of isolated fat cells. II. The similar effects of phospholipase C (Clostridium perfringens alpha toxin) and of insulin on glucose and amino acid metabolism. J Biol Chem. 1966 Jan 10;241(1):130–139. [PubMed] [Google Scholar]
  11. STREFFER C., WILLIAMSON D. H. THE EFFECT OF CALCIUM IONS ON THE LEAKAGE OF PROTEIN AND ENZYMES FROM RAT-LIVER SLICES. Biochem J. 1965 May;95:552–560. doi: 10.1042/bj0950552. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. WOOL I. G., KRAHL M. E. Incorporation of C14-amino acids into protein of isolated diaphragms: an effect of insulin independent of glucose entry. Am J Physiol. 1959 May;196(5):961–964. doi: 10.1152/ajplegacy.1959.196.5.961. [DOI] [PubMed] [Google Scholar]
  13. Wool I. G., Rampersad O. R., Moyer A. N. Effect of insulin and diabetes on protein synthesis by ribosomes from heart muscle. Significance for theories of the hormone's mechanism of action. Am J Med. 1966 May;40(5):716–723. doi: 10.1016/0002-9343(66)90152-5. [DOI] [PubMed] [Google Scholar]

Articles from The Journal of General Physiology are provided here courtesy of The Rockefeller University Press

RESOURCES