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. 1969 Sep 1;54(3):310–330. doi: 10.1085/jgp.54.3.310

The Ventral Photoreceptor Cells of Limulus

II. The basic photoresponse

Ronald Millecchia 1, Alexander Mauro 1
PMCID: PMC2225928  PMID: 5806592

Abstract

The ventral photoreceptors of Limulus polyphemus are unipolar cells with large, ellipsoidal somas located long both "lateral olfactory nerves." As a consequence of their size and location, the cells are easily impaled with microelectrodes. The cells have an average resting potential of -48 mv. The resting potential is a function of the external concentration of K. When the cell is illuminated, it gives rise to the typical "receptor potential" seen in most invertebrate photoreceptors which consists of a transient phase followed by a maintained phase of depolarization. The amplitude of the transient phase depends on both the state of adaptation of the cell and the intensity of the illumination, while the amplitude of the maintained phase depends only on the intensity of the illumination. The over-all size of the receptor potential depends on the external concentration of Na, e.g. in sodium-free seawater the receptor potential is markedly reduced, but not abolished. On the other hand lowering the Ca concentration produces a marked enhancement of both components of the response, but predominantly of the steady-state component. Slow potential fluctuations are seen in the dark-adapted cell when it is illuminated with a low intensity light. A spike-like regenerative process can be evoked by either the receptor potential or a current applied via a microelectrode. No evidence of impulse activity has been found in the axons of these cells. The ventral photoreceptor cell has many properties in common with a variety of retinular cells and therefore should serve as a convenient model of the primary receptor cell in many invertebrate eyes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ADOLPH A. R. SPONTANEOUS SLOW POTENTIAL FLUCTUATIONS IN THE LIMULUS PHOTORECEPTOR. J Gen Physiol. 1964 Nov;48:297–322. doi: 10.1085/jgp.48.2.297. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. ADRIAN R. H. The effect of internal and external potassium concentration on the membrane potential of frog muscle. J Physiol. 1956 Sep 27;133(3):631–658. doi: 10.1113/jphysiol.1956.sp005615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baumann F. Slow and spike potentials recorded from retinula cells of the honeybee drone in response to light. J Gen Physiol. 1968 Dec;52(6):855–875. doi: 10.1085/jgp.52.6.855. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Behrens M. E., Wulff V. J. Light-initiated responses of retinula and eccentric cells in the Limulus lateral eye. J Gen Physiol. 1965 Jul;48(6):1081–1093. doi: 10.1085/jgp.48.6.1081. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Benolken R. M. Regenerative transducing properties of a graded visual response. Cold Spring Harb Symp Quant Biol. 1965;30:445–450. doi: 10.1101/sqb.1965.030.01.043. [DOI] [PubMed] [Google Scholar]
  6. Chapman R. M., Lall A. B. Electroretinogram characteristics and the spectral mechanisms of the median ocellus and the lateral eye in Limulus polyphemus. J Gen Physiol. 1967 Oct;50(9):2267–2287. doi: 10.1085/jgp.50.9.2267. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Clark A. W., Millecchia R., Mauro A. The ventral photoreceptor cells of Limulus. I. The microanatomy. J Gen Physiol. 1969 Sep;54(3):289–309. doi: 10.1085/jgp.54.3.289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. DIAMOND J., GRAY J. A., INMAN D. R. The relation between receptor potentials and the concentration of sodium ions. J Physiol. 1958 Jul 14;142(2):382–394. doi: 10.1113/jphysiol.1958.sp006024. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. EDWARDS C., TERZUOLO C. A., WASHIZU H. THE EFFECT OF CHANGES OF THE IONIC ENVIRONMENT UPON AN ISOLATED CRUSTACEAN SENSORY NEURON. J Neurophysiol. 1963 Nov;26:948–957. doi: 10.1152/jn.1963.26.6.948. [DOI] [PubMed] [Google Scholar]
  10. FUORTES M. G., HODGKIN A. L. CHANGES IN TIME SCALE AND SENSITIVITY IN THE OMMATIDIA OF LIMULUS. J Physiol. 1964 Aug;172:239–263. doi: 10.1113/jphysiol.1964.sp007415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. FUORTES M. G., POGGIO G. F. Transient responses to sudden illumination in cells of the eye of Limulus. J Gen Physiol. 1963 Jan;46:435–452. doi: 10.1085/jgp.46.3.435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. FUORTES M. G., YEANDLE S. PROBABILITY OF OCCURRENCE OF DISCRETE POTENTIAL WAVES IN THE EYE OF LIMULUS. J Gen Physiol. 1964 Jan;47:443–463. doi: 10.1085/jgp.47.3.443. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. HARTLINE H. K., WAGNER H. G., MACNICHOL E. F., Jr The peripheral origin of nervous activity in the visual system. Cold Spring Harb Symp Quant Biol. 1952;17:125–141. doi: 10.1101/sqb.1952.017.01.013. [DOI] [PubMed] [Google Scholar]
  14. HUBBARD R., WALD G. Visual pigment of the horseshoe crab, Limulus polyphemus. Nature. 1960 Apr 16;186:212–215. doi: 10.1038/186212b0. [DOI] [PubMed] [Google Scholar]
  15. HUXLEY A. F., STAMPFLI R. Effect of potassium and sodium on resting and action potentials of single myelinated nerve fibers. J Physiol. 1951 Feb;112(3-4):496–508. doi: 10.1113/jphysiol.1951.sp004546. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. JULIAN F. J., MOORE J. W., GOLDMAN D. E. Membrane potentials of the lobster giant axon obtained by use of the sucrose-gap technique. J Gen Physiol. 1962 Jul;45:1195–1216. doi: 10.1085/jgp.45.6.1195. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. KIKUCHI R., NAITO K., TANAKA I. Effect of sodium and potassium ions on the electrical activity of single cells in the lateral eye of the horseshoe crab. J Physiol. 1962 May;161:319–343. doi: 10.1113/jphysiol.1962.sp006889. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kuffler S. W., Nicholls J. G., Orkand R. K. Physiological properties of glial cells in the central nervous system of amphibia. J Neurophysiol. 1966 Jul;29(4):768–787. doi: 10.1152/jn.1966.29.4.768. [DOI] [PubMed] [Google Scholar]
  19. LING G., GERARD R. W. External potassium and the membrane potential of single muscle fibers. Nature. 1950 Jan 21;165(4186):113–113. doi: 10.1038/165113a0. [DOI] [PubMed] [Google Scholar]
  20. Millecchia R., Bradbury J., Mauro A. Simple photoreceptors in Limulus polyphemus. Science. 1966 Dec 2;154(3753):1199–1201. doi: 10.1126/science.154.3753.1199. [DOI] [PubMed] [Google Scholar]
  21. Millecchia R., Mauro A. The ventral photoreceptor cells of Limulus. 3. A voltage-clamp study. J Gen Physiol. 1969 Sep;54(3):331–351. doi: 10.1085/jgp.54.3.331. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Murray G. C. Intracellular absorption difference spectrum of Limulus extra-ocular photolabile pigment. Science. 1966 Dec 2;154(3753):1182–1183. doi: 10.1126/science.154.3753.1182. [DOI] [PubMed] [Google Scholar]
  23. NARAHASHI T., MOORE J. W., SCOTT W. R. TETRODOTOXIN BLOCKAGE OF SODIUM CONDUCTANCE INCREASE IN LOBSTER GIANT AXONS. J Gen Physiol. 1964 May;47:965–974. doi: 10.1085/jgp.47.5.965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Ripley S. H., Bush B. M., Roberts A. Crab muscle receptor which responds without impulses. Nature. 1968 Jun 22;218(5147):1170–1171. doi: 10.1038/2181170a0. [DOI] [PubMed] [Google Scholar]
  25. Treherne J. E., Maddrell S. H. Axonal function and ionic regulation in the central nervous system of a phytophagous insect (Carausius morosus). J Exp Biol. 1967 Oct;47(2):235–247. doi: 10.1242/jeb.47.2.235. [DOI] [PubMed] [Google Scholar]
  26. WALD G., KRAININ J. M. THE MEDIAN EYE OF LIMULUS: AN ULTRAVIOLET RECEPTOR. Proc Natl Acad Sci U S A. 1963 Dec;50:1011–1017. doi: 10.1073/pnas.50.6.1011. [DOI] [PMC free article] [PubMed] [Google Scholar]

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