Abstract
We measured the internal potassium activity, ai K, and membrane potential, Em, simultaneously in 111 R2 giant neurons of Aplysia californica. ai K was 165.3 ± 3.4 mM, Em was -47.8 ± 0.9 mv, and E K calculated using the Nernst equation was -76.9 ± 0.05 mv. Such values were maintained for as long as 6 hr of continuous recording in untreated cells, ai K fell exponentially after the following treatments: cooling to 0.5°–4°C, ouabain, zero external potassium, 2,4-dinitrophenol, and cyanide. The effects of cooling and zero potassium were reversible. Potassium permeability was calculated from net potassium flux using the constant field equation and ranged from 2.6 to 18.5 x 10-8 cm/sec. We conclude that potassium is actively transported into this neuron against a 30–40 mv electrochemical gradient.
Full Text
The Full Text of this article is available as a PDF (735.8 KB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Brown A. M., Sutton R. B., Walker J. L., Jr Increased chloride conductance as the proximate cause of hydrogen ion concentration effects in Aplysia neurons. J Gen Physiol. 1970 Nov;56(5):559–582. doi: 10.1085/jgp.56.5.559. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CONWAY E. J. NEW LIGHT ON THE ACTIVE TRANSPORT OF SODIUM IONS FROM SKELETAL MUSCLE. Fed Proc. 1964 May-Jun;23:680–688. [PubMed] [Google Scholar]
- Carpenter D. O., Alving B. O. A contribution of an electrogenic Na+ pump to membrane potential in Aplysia neurons. J Gen Physiol. 1968 Jul;52(1):1–21. doi: 10.1085/jgp.52.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Carpenter D. O., Hovey M. M., Bak A. F. Intracellular conductance of Aplysia neurons and squid axon as determined by a new technique. Int J Neurosci. 1971 Jul;2(1):35–48. doi: 10.3109/00207457109146991. [DOI] [PubMed] [Google Scholar]
- De Weer P. Effects of intracellular adenosine-5'-diphosphate and orthophosphate on the sensitivity of sodium efflux from squid axon to external sodium and potassium. J Gen Physiol. 1970 Nov;56(5):583–620. doi: 10.1085/jgp.56.5.583. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GLYNN I. M. THE ACTION OF CARDIAC GLYCOSIDES ON ION MOVEMENTS. Pharmacol Rev. 1964 Dec;16:381–407. [PubMed] [Google Scholar]
- Gorman A. L., Marmor M. F. Contributions of the sodium pump and ionic gradients to the membrane potential of a molluscan neurone. J Physiol. 1970 Nov;210(4):897–917. doi: 10.1113/jphysiol.1970.sp009248. [DOI] [PMC free article] [PubMed] [Google Scholar]
- HODGKIN A. L., HOROWICZ P. The influence of potassium and chloride ions on the membrane potential of single muscle fibres. J Physiol. 1959 Oct;148:127–160. doi: 10.1113/jphysiol.1959.sp006278. [DOI] [PMC free article] [PubMed] [Google Scholar]
- KERKUT G. A., THOMAS R. C. AN ELECTROGENIC SODIUM PUMP IN SNAIL NERVE CELLS. Comp Biochem Physiol. 1965 Jan;14:167–183. doi: 10.1016/0010-406x(65)90017-4. [DOI] [PubMed] [Google Scholar]
- Katchalsky A., Spangler R. Dynamics of membrane processes. Q Rev Biophys. 1968 Jun;1(2):127–175. doi: 10.1017/s0033583500000524. [DOI] [PubMed] [Google Scholar]
- Kehoe J. S., Ascher P. Re-evaluation of the synaptic activation of an electrogenic sodium pump. Nature. 1970 Feb 28;225(5235):820–823. doi: 10.1038/225820a0. [DOI] [PubMed] [Google Scholar]
- Kunze D. L., Brown A. M. Internal potassium and chloride activities and the effects of acetylcholine on identifiable Aplysia neurones. Nat New Biol. 1971 Feb 24;229(8):229–231. doi: 10.1038/newbio229229a0. [DOI] [PubMed] [Google Scholar]
- Lorenzo Marchiafava P. The effect of temperature change on membrane potential and conductance in Aplysia giant nerve cell. Comp Biochem Physiol. 1970 Jun 15;34(4):847–852. doi: 10.1016/0010-406x(70)91007-8. [DOI] [PubMed] [Google Scholar]
- Noble D. Applications of Hodgkin-Huxley equations to excitable tissues. Physiol Rev. 1966 Jan;46(1):1–50. doi: 10.1152/physrev.1966.46.1.1. [DOI] [PubMed] [Google Scholar]
- Russell J. M., Brown A. M. Active transport of chloride by the giant neuron of the Aplysia abdominal ganglion. J Gen Physiol. 1972 Nov;60(5):499–518. doi: 10.1085/jgp.60.5.499. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Russell J. M., Brown A. M. Active transport of potassium and chloride in an identifiable molluscan neuron. Science. 1972 Mar 31;175(4029):1475–1477. doi: 10.1126/science.175.4029.1475. [DOI] [PubMed] [Google Scholar]
- SHANES A. M., BERMAN M. D. Kinetics of ion movement in the squid giant axon. J Gen Physiol. 1955 Nov 20;39(2):279–300. doi: 10.1085/jgp.39.2.279. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sato M., Austin G., Yai H., Maruhashi J. The ionic permeability changes during acetylcholine-induced responses of Aplysia ganglion cells. J Gen Physiol. 1968 Mar;51(3):321–345. doi: 10.1085/jgp.51.3.321. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thomas R. C. Membrane current and intracellular sodium changes in a snail neurone during extrusion of injected sodium. J Physiol. 1969 Apr;201(2):495–514. doi: 10.1113/jphysiol.1969.sp008769. [DOI] [PMC free article] [PubMed] [Google Scholar]