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. 1973 Oct 1;62(4):407–429. doi: 10.1085/jgp.62.4.407

A Micropuncture Investigation of the Whole Tissue Mechanism of Electrolyte Secretion by the In Vitro Rabbit Pancreas

C H Swanson 1, A K Solomon 1
PMCID: PMC2226128  PMID: 4755848

Abstract

Micropuncture techniques have been used to examine electrolyte secretion by the in vitro rabbit pancreas. The concentration profiles of the major secreted ions and digestive protein and the electrical potential profile within the pancreatic ductal system have been determined during spontaneous and secretin-stimulated secretion. The active transport of both Na and HCO3 are the rate-controlling steps in primary secretion. Spontaneous secretion is produced primarily within the intralobular ducts. The anion composition of this primary secretion depends on the secretion rate with HCO3 ranging from about 70 meq/liter at low rates to about 110 meq/liter at high rates. With secretin stimulation the smaller extralobular ducts also secrete and this extralobular secretion has a higher HCO2 content than that of the intralobular secretion. In the main collecting duct the anion composition of the juice is modified further by Cl-HCO3 exchange which appears to be a passive process depending on the average residence time of the juice in the main duct.

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Selected References

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