Abstract
The permeability coefficients of dog red cell membrane to tritiated water and to a series of[14C]amides have been deduced from bulk diffusion measurements through a "tissue" composed of packed red cells. Red cells were packed by centrifugation inside polyethylene tubing. The red cell column was pulsed at one end with radiolabeled solute and diffusion was allowed to proceed for several hours. The distribution of radioactivity along the red cell column was measured by sequential slicing and counting, and the diffusion coefficient was determined by a simple plotting technique, assuming a one-dimensional diffusional model. In order to derive the red cell membrane permeability coefficient from the bulk diffusion coefficient, the red cells were assumed to be packed in a regular manner approximating closely spaced parallelopipeds. The local steady-state diffusional flux was idealized as a one-dimensional intracellular pathway in parallel with a one-dimensional extracellular pathway with solute exchange occurring within the series pathway and between the pathways. The diffusion coefficients in the intracellular and extracellular pathways were estimated from bulk diffusion measurements through concentrated hemoglobin solutions and plasma, respectively; while the volume of the extracellular pathway was determined using radiolabeled sucrose. The membrane permeability coefficients were in satisfactory agreement with the data of Sha'afi, R. I., C. M. Gary-Bobo, and A. K. Solomon (1971. J. Gen. Physiol. 58:238) obtained by a rapid-reaction technique. The method is simple and particularly well suited for rapidly permeating solutes.
Full Text
The Full Text of this article is available as a PDF (1.3 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- BORUN E. R., FIGUEROA W. G., PERRY S. M. The distribution of Fe59 tagged human erythrocytes in centrifuged specimens as a function of cell age. J Clin Invest. 1957 May;36(5):676–679. doi: 10.1172/JCI103468. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Chinard F. P., Thaw C. N., Delea A. C., Perl W. Intrarenal volumes of distribution and relative diffusion coefficients of monohydric alcohols. Circ Res. 1969 Sep;25(3):343–357. doi: 10.1161/01.res.25.3.343. [DOI] [PubMed] [Google Scholar]
- Gary-Bobo C. M., Lange Y., Rigaud J. L. Water diffusion in lecithin-water and lecithin-cholesterol-water lamellar phases at 22 degrees. Biochim Biophys Acta. 1971 Mar 9;233(1):243–246. doi: 10.1016/0005-2736(71)90378-6. [DOI] [PubMed] [Google Scholar]
- Gary-Bobo C. M., Solomon A. K. Properties of hemoglobin solutions in red cells. J Gen Physiol. 1968 Nov;52(5):825–853. doi: 10.1085/jgp.52.5.825. [DOI] [PMC free article] [PubMed] [Google Scholar]
- JOYCE C. R. Uptake of potassium and sodium by parts of packed human blood cell column. Q J Exp Physiol Cogn Med Sci. 1958 Jul;43(3):299–309. doi: 10.1113/expphysiol.1958.sp001333. [DOI] [PubMed] [Google Scholar]
- KEITER H. G., BERMAN H., JONES H., MACLACHLAN E. The chemical composition of normal human red blood cells, including variability among centrifuged cells. Blood. 1955 Apr;10(4):370–376. [PubMed] [Google Scholar]
- KETY S. S. The theory and applications of the exchange of inert gas at the lungs and tissues. Pharmacol Rev. 1951 Mar;3(1):1–41. [PubMed] [Google Scholar]
- Keller K. H., Friedlander S. K. The steady-state transport of oxygen through hemoglobin solutions. J Gen Physiol. 1966 Mar;49(4):663–679. doi: 10.1085/jgp.49.4.663. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Krogh A. The rate of diffusion of gases through animal tissues, with some remarks on the coefficient of invasion. J Physiol. 1919 May 20;52(6):391–408. doi: 10.1113/jphysiol.1919.sp001838. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Naccache P., Sha'afi R. I. Patterns of nonelectrolyte permeability in human red blood cell membrane. J Gen Physiol. 1973 Dec;62(6):714–736. doi: 10.1085/jgp.62.6.714. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PAGANELLI C. V., SOLOMON A. K. The rate of exchange of tritiated water across the human red cell membrane. J Gen Physiol. 1957 Nov 20;41(2):259–277. doi: 10.1085/jgp.41.2.259. [DOI] [PMC free article] [PubMed] [Google Scholar]
- RUDBACH J. A., RIBI E., MILNER K. C. REACTIVATION OF PAPAIN-TREATED ENDOTOXIN. Proc Soc Exp Biol Med. 1965 May;119:115–118. doi: 10.3181/00379727-119-30112. [DOI] [PubMed] [Google Scholar]
- SCHANTZ E. J., LAUFFER M. A. Diffusion measurements in agar gel. Biochemistry. 1962 Jul;1:658–663. doi: 10.1021/bi00910a019. [DOI] [PubMed] [Google Scholar]
- SIDEL V. W., SOLOMON A. K. Entrance of water into human red cells under an osmotic pressure gradient. J Gen Physiol. 1957 Nov 20;41(2):243–257. doi: 10.1085/jgp.41.2.243. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sha'afi R. I., Gary-Bobo C. M., Solomon A. K. Permeability of red cell membranes to small hydrophilic and lipophilic solutes. J Gen Physiol. 1971 Sep;58(3):238–258. doi: 10.1085/jgp.58.3.238. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sha'afi R. I., Rich G. T., Mikulecky D. C., Solomon A. K. Determination of urea permeability in red cells by minimum method. A test of the phenomenological equations. J Gen Physiol. 1970 Apr;55(4):427–450. doi: 10.1085/jgp.55.4.427. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Solomon A. K. Properties of water in red cell and synthetic membranes. Biomembranes. 1972;3:299–330. doi: 10.1007/978-1-4684-0961-1_21. [DOI] [PubMed] [Google Scholar]
- Vieira F. L., Sha'afi R. I., Solomon A. K. The state of water in human and dog red cell membranes. J Gen Physiol. 1970 Apr;55(4):451–466. doi: 10.1085/jgp.55.4.451. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WESTERMAN M. P., PIERCE L. E., JENSEN W. N. ERYTHROCYTE LIPIDS: A COMPARISON OF NORMAL YOUNG AND NORMAL OLD POPULATIONS. J Lab Clin Med. 1963 Sep;62:394–400. [PubMed] [Google Scholar]