Skip to main content
PMC home page
British Journal of Cancer logoLink to British Journal of Cancer
. 1997;76(7):829–835. doi: 10.1038/bjc.1997.471

Complexity of expression of the intermediate filaments of six new human ovarian carcinoma cell lines: new expression of cytokeratin 20.

T Yanagibashi 1, I Gorai 1, T Nakazawa 1, E Miyagi 1, F Hirahara 1, H Kitamura 1, H Minaguchi 1
PMCID: PMC2228076  PMID: 9328139

Abstract

Six permanent human ovarian carcinoma cell lines (OVISE, OVTOKO, OVMANA and OVSAYO from clear cell adenocarcinoma, and OVSAHO and OVKATE from serous papillary adenocarcinoma) were established from solid tumours. The cell lines have been in culture for 5-8 years, the passage number varying from 62 to 246. Immunohistochemical analysis has shown that five of the six cell lines express at least six cytokeratin (CK) polypeptides. OVISE and OVSAYO expressed CKs 6, 7, 8, 18, 19 and 15 and/or 16. OVTOKO was positive for CKs 7, 8, 18, 19 and 15 and/or 16. OVSAHO expressed CKs 6, 7, 8, 14, 18, 19 and 15 and/or 16. OVMANA expressed CKs 6, 7, 8, 18, 19, 20 and 15 and/or 16. OVKATE expressed CKs 6, 7, 8, 13, 17, 18, 19, 20 and 15 and/or 16. The expression of CK7, additional expression of vimentin, and clinical and histopathological findings enabled us to confirm that six cell lines had been established from primary ovarian cancers. Two of the six cell lines were positive for CK20, although CK20 was not expressed in the original tumours. The heterotransplanted tumours produced by CK20-positive cells also expressed CK20. This is the first report of ovarian carcinoma cell lines that express CK20 irrespective of their histological type. CK20 has been found in all colon carcinoma cell lines, but only in the mucinous type of ovarian tumours. These new ovarian carcinoma cell lines will therefore provide a relevant experimental system for elucidating the regulatory control mechanisms of intermediate filament expression.

Full text

PDF
829

Images in this article

832Figure 1
on p.832
832Figure 2
on p.832
832Figure 3
on p.832

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berezowski K., Stastny J. F., Kornstein M. J. Cytokeratins 7 and 20 and carcinoembryonic antigen in ovarian and colonic carcinoma. Mod Pathol. 1996 Apr;9(4):426–429. [PubMed] [Google Scholar]
  2. Buick R. N., Pullano R., Trent J. M. Comparative properties of five human ovarian adenocarcinoma cell lines. Cancer Res. 1985 Aug;45(8):3668–3676. [PubMed] [Google Scholar]
  3. Coggi G., Dell'Orto P., Braidotti P., Coggi A., Viale G. Coexpression of intermediate filaments in normal and neoplastic human tissues: a reappraisal. Ultrastruct Pathol. 1989 Sep-Dec;13(5-6):501–514. doi: 10.3109/01913128909074533. [DOI] [PubMed] [Google Scholar]
  4. Cordell J. L., Falini B., Erber W. N., Ghosh A. K., Abdulaziz Z., MacDonald S., Pulford K. A., Stein H., Mason D. Y. Immunoenzymatic labeling of monoclonal antibodies using immune complexes of alkaline phosphatase and monoclonal anti-alkaline phosphatase (APAAP complexes). J Histochem Cytochem. 1984 Feb;32(2):219–229. doi: 10.1177/32.2.6198355. [DOI] [PubMed] [Google Scholar]
  5. Crickard K., Niedbala M. J., Crickard U., Yoonessi M., Sandberg A. A., Okuyama K., Bernacki R. J., Satchidanand S. K. Characterization of human ovarian and endometrial carcinoma cell lines established on extracellular matrix. Gynecol Oncol. 1989 Feb;32(2):163–173. doi: 10.1016/s0090-8258(89)80028-9. [DOI] [PubMed] [Google Scholar]
  6. Doran T. I., Vidrich A., Sun T. T. Intrinsic and extrinsic regulation of the differentiation of skin, corneal and esophageal epithelial cells. Cell. 1980 Nov;22(1 Pt 1):17–25. doi: 10.1016/0092-8674(80)90150-6. [DOI] [PubMed] [Google Scholar]
  7. Fuchs E., Green H. Regulation of terminal differentiation of cultured human keratinocytes by vitamin A. Cell. 1981 Sep;25(3):617–625. doi: 10.1016/0092-8674(81)90169-0. [DOI] [PubMed] [Google Scholar]
  8. Fuchs E., Green H. The expression of keratin genes in epidermis and cultured epidermal cells. Cell. 1978 Nov;15(3):887–897. doi: 10.1016/0092-8674(78)90273-8. [DOI] [PubMed] [Google Scholar]
  9. Fuchtner C., Emma D. A., Manetta A., Gamboa G., Bernstein R., Liao S. Y. Characterization of a human ovarian carcinoma cell line: UCI 101. Gynecol Oncol. 1993 Feb;48(2):203–209. doi: 10.1006/gyno.1993.1034. [DOI] [PubMed] [Google Scholar]
  10. Giorno R. A comparison of two immunoperoxidase staining methods based on the avidin-biotin interaction. Diagn Immunol. 1984;2(3):161–166. [PubMed] [Google Scholar]
  11. Gorai I., Doi C., Minaguchi H. Establishment and characterization of carcinosarcoma cell line of the human uterus. Cancer. 1993 Feb 1;71(3):775–786. doi: 10.1002/1097-0142(19930201)71:3<775::aid-cncr2820710321>3.0.co;2-c. [DOI] [PubMed] [Google Scholar]
  12. Gorai I., Nakazawa T., Miyagi E., Hirahara F., Nagashima Y., Minaguchi H. Establishment and characterization of two human ovarian clear cell adenocarcinoma lines from metastatic lesions with different properties. Gynecol Oncol. 1995 Apr;57(1):33–46. doi: 10.1006/gyno.1995.1097. [DOI] [PubMed] [Google Scholar]
  13. Hill B. T., Whelan R. D., Gibby E. M., Sheer D., Hosking L. K., Shellard S. A., Rupniak H. T. Establishment and characterisation of three new human ovarian carcinoma cell lines and initial evaluation of their potential in experimental chemotherapy studies. Int J Cancer. 1987 Feb 15;39(2):219–225. doi: 10.1002/ijc.2910390216. [DOI] [PubMed] [Google Scholar]
  14. Hills C. A., Kelland L. R., Abel G., Siracky J., Wilson A. P., Harrap K. R. Biological properties of ten human ovarian carcinoma cell lines: calibration in vitro against four platinum complexes. Br J Cancer. 1989 Apr;59(4):527–534. doi: 10.1038/bjc.1989.108. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Horowitz A. T., Treves A. J., Voss R., Okon E., Fuks Z., Davidson L., Biran S. A new human ovarian carcinoma cell line: establishment and analysis of tumor-associated markers. Oncology. 1985;42(5):332–337. doi: 10.1159/000226056. [DOI] [PubMed] [Google Scholar]
  16. Knapp A. C., Franke W. W. Spontaneous losses of control of cytokeratin gene expression in transformed, non-epithelial human cells occurring at different levels of regulation. Cell. 1989 Oct 6;59(1):67–79. doi: 10.1016/0092-8674(89)90870-2. [DOI] [PubMed] [Google Scholar]
  17. Langdon S. P., Lawrie S. S., Hay F. G., Hawkes M. M., McDonald A., Hayward I. P., Schol D. J., Hilgers J., Leonard R. C., Smyth J. F. Characterization and properties of nine human ovarian adenocarcinoma cell lines. Cancer Res. 1988 Nov 1;48(21):6166–6172. [PubMed] [Google Scholar]
  18. Maeda T., Kajiyama K., Adachi E., Takenaka K., Sugimachi K., Tsuneyoshi M. The expression of cytokeratins 7, 19, and 20 in primary and metastatic carcinomas of the liver. Mod Pathol. 1996 Sep;9(9):901–909. [PubMed] [Google Scholar]
  19. Malik N. J., Daymon M. E. Improved double immunoenzyme labeling using alkaline phosphatase and horseradish peroxidase. J Clin Pathol. 1982 Oct;35(10):1092–1094. doi: 10.1136/jcp.35.10.1092. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Moll R., Franke W. W., Schiller D. L., Geiger B., Krepler R. The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells. Cell. 1982 Nov;31(1):11–24. doi: 10.1016/0092-8674(82)90400-7. [DOI] [PubMed] [Google Scholar]
  21. Moll R., Löwe A., Laufer J., Franke W. W. Cytokeratin 20 in human carcinomas. A new histodiagnostic marker detected by monoclonal antibodies. Am J Pathol. 1992 Feb;140(2):427–447. [PMC free article] [PubMed] [Google Scholar]
  22. Moll R., Pitz S., Levy R., Weikel W., Franke W. W., Czernobilsky B. Complexity of expression of intermediate filament proteins, including glial filament protein, in endometrial and ovarian adenocarcinomas. Hum Pathol. 1991 Oct;22(10):989–1001. doi: 10.1016/0046-8177(91)90007-c. [DOI] [PubMed] [Google Scholar]
  23. Moll R., Schiller D. L., Franke W. W. Identification of protein IT of the intestinal cytoskeleton as a novel type I cytokeratin with unusual properties and expression patterns. J Cell Biol. 1990 Aug;111(2):567–580. doi: 10.1083/jcb.111.2.567. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Morimitsu Y., Yano H., Kojiro M. Morphologic characteristics, proliferation, and tumor marker expression of two human ovarian carcinoma cell lines in three-dimensional culture. Gynecol Oncol. 1993 Feb;48(2):155–164. doi: 10.1006/gyno.1993.1027. [DOI] [PubMed] [Google Scholar]
  25. Möbus V. J., Moll R., Gerharz C. D., Kieback D. G., Weikel W., Hoffmann G., Kreienberg R. Establishment of new ovarian and colon carcinoma cell lines: differentiation is only possible by cytokeratin analysis. Br J Cancer. 1994 Mar;69(3):422–428. doi: 10.1038/bjc.1994.78. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Möbus V., Gerharz C. D., Press U., Moll R., Beck T., Mellin W., Pollow K., Knapstein P. G., Kreienberg R. Morphological, immunohistochemical and biochemical characterization of 6 newly established human ovarian carcinoma cell lines. Int J Cancer. 1992 Aug 19;52(1):76–84. doi: 10.1002/ijc.2910520115. [DOI] [PubMed] [Google Scholar]
  27. Ramaekers F., van Niekerk C., Poels L., Schaafsma E., Huijsmans A., Robben H., Schaart G., Vooijs P. Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas. Am J Pathol. 1990 Mar;136(3):641–655. [PMC free article] [PubMed] [Google Scholar]
  28. Sheer D., Sheppard D. M., Gorman P. A., Ward B., Whelan R. D., Hill B. T. Cytogenetic analysis of four human ovarian carcinoma cell lines. Cancer Genet Cytogenet. 1987 Jun;26(2):339–349. doi: 10.1016/0165-4608(87)90068-9. [DOI] [PubMed] [Google Scholar]
  29. Sun T. T., Green H. Keratin filaments of cultured human epidermal cells. Formation of intermolecular disulfide bonds during terminal differentiation. J Biol Chem. 1978 Mar 25;253(6):2053–2060. [PubMed] [Google Scholar]
  30. Ueda G., Sawada M., Ogawa H., Tanizawa O., Tsujimoto M. Immunohistochemical study of cytokeratin 7 for the differential diagnosis of adenocarcinomas in the ovary. Gynecol Oncol. 1993 Nov;51(2):219–223. doi: 10.1006/gyno.1993.1276. [DOI] [PubMed] [Google Scholar]
  31. Van Niekerk C. C., Ramaekers F. C., Hanselaar A. G., Aldeweireldt J., Poels L. G. Changes in expression of differentiation markers between normal ovarian cells and derived tumors. Am J Pathol. 1993 Jan;142(1):157–177. [PMC free article] [PubMed] [Google Scholar]
  32. Viale G., Gambacorta M., Dell'Orto P., Coggi G. Coexpression of cytokeratins and vimentin in common epithelial tumours of the ovary: an immunocytochemical study of eighty-three cases. Virchows Arch A Pathol Anat Histopathol. 1988;413(2):91–101. doi: 10.1007/BF00749670. [DOI] [PubMed] [Google Scholar]
  33. Wauters C. C., Smedts F., Gerrits L. G., Bosman F. T., Ramaekers F. C. Keratins 7 and 20 as diagnostic markers of carcinomas metastatic to the ovary. Hum Pathol. 1995 Aug;26(8):852–855. doi: 10.1016/0046-8177(95)90006-3. [DOI] [PubMed] [Google Scholar]
  34. Wolf C. R., Hayward I. P., Lawrie S. S., Buckton K., McIntyre M. A., Adams D. J., Lewis A. D., Scott A. R., Smyth J. F. Cellular heterogeneity and drug resistance in two ovarian adenocarcinoma cell lines derived from a single patient. Int J Cancer. 1987 Jun 15;39(6):695–702. doi: 10.1002/ijc.2910390607. [DOI] [PubMed] [Google Scholar]
  35. Wong W. S., Wong Y. F., Ng Y. T., Huang P. D., Chew E. C., Ho T. H., Chang M. Z. Establishment and characterization of a new human cell line derived from ovarian clear cell carcinoma. Gynecol Oncol. 1990 Jul;38(1):37–45. doi: 10.1016/0090-8258(90)90008-9. [DOI] [PubMed] [Google Scholar]
  36. Wu Y. J., Rheinwald J. G. A new small (40 kd) keratin filament protein made by some cultured human squamous cell carcinomas. Cell. 1981 Sep;25(3):627–635. doi: 10.1016/0092-8674(81)90170-7. [DOI] [PubMed] [Google Scholar]
  37. van Niekerk C. C., Poels L. G., Jap P. H., Smeets D. F., Thomas C. M., Ramaekers F. C., Vooijs G. P. Characterization of a human ovarian carcinoma cell line, OTN 14, derived from a mucinous cystadenocarcinoma. Int J Cancer. 1988 Jul 15;42(1):104–111. doi: 10.1002/ijc.2910420120. [DOI] [PubMed] [Google Scholar]
  38. van den Berg-Bakker C. A., Hagemeijer A., Franken-Postma E. M., Smit V. T., Kuppen P. J., van Ravenswaay Claasen H. H., Cornelisse C. J., Schrier P. I. Establishment and characterization of 7 ovarian carcinoma cell lines and one granulosa tumor cell line: growth features and cytogenetics. Int J Cancer. 1993 Feb 20;53(4):613–620. doi: 10.1002/ijc.2910530415. [DOI] [PubMed] [Google Scholar]

Articles from British Journal of Cancer are provided here courtesy of Cancer Research UK

RESOURCES