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. 1979 Jul 1;74(1):85–104. doi: 10.1085/jgp.74.1.85

The active tension-length curve of vascular smooth muscle related to its cellular components

PMCID: PMC2228486  PMID: 479823

Abstract

The active and passive isometric tension-length (internal circumference) relation of vascular smooth muscle has been investigated using a 100-200-micron lumen diameter artery from the rat mesenteric bed. Conditions were established under which maximal activation was obtained at all lengths. Below L0 (the length at which maximum tension, delta T0, was developed) the active tension fell with decreasing length along a line which extrapolated to 0.38 L0; below 1.1 L0 the relation was reversible regardless of the protocol used. Above L0 the active tension fell linearly with increasing length along a line which extrapolated to zero tension at 1.82 L0. At the longer lengths investigated (up to 1.6 L0) the passive tension upon which the active responses were superimposed was as high as 4.4 delta T0. However, measurements of the dynamic characteristics of the preparation (with a time resolution of 2 ms) suggest that the active tension measured is nevertheless a measure of the active properties of the contractile apparatus. Direct light microscopic observation of the effect of length change on the cells within the walls of the preparation showed that changes in vessel length produced, on average, the same percentage change in cell length. Histological examination showed no signs of cell destruction following large extensions. The results suggest that the decrease in tension with extension above L0 is due to changes in the properties of the contractile apparatus, rather than to cellular damage.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashton F. T., Somlyo A. V., Somlyo A. P. The contractile apparatus of vascular smooth muscle: intermediate high voltage stereo electron microscopy. J Mol Biol. 1975 Oct 15;98(1):17–29. doi: 10.1016/s0022-2836(75)80098-2. [DOI] [PubMed] [Google Scholar]
  2. Bagby R. M., Fisher B. A. Graded contractions in muscle strips and single cells from Bufo marinus stomach. Am J Physiol. 1973 Jul;225(1):105–109. doi: 10.1152/ajplegacy.1973.225.1.105. [DOI] [PubMed] [Google Scholar]
  3. Bressler B. H., Clinch N. F. Cross bridges as the major source of compliance in contracting skeletal muscle. Nature. 1975 Jul 17;256(5514):221–222. doi: 10.1038/256221a0. [DOI] [PubMed] [Google Scholar]
  4. Bressler B. H., Clinch N. F. The compliance of contracting skeletal muscle. J Physiol. 1974 Mar;237(3):477–493. doi: 10.1113/jphysiol.1974.sp010493. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cornelius F., Lowy J. Tension-length behaviour of a molluscan smooth muscle related to filament organisation. Acta Physiol Scand. 1978 Feb;102(2):167–180. doi: 10.1111/j.1748-1716.1978.tb06060.x. [DOI] [PubMed] [Google Scholar]
  6. Fay F. S. Isometric contractile properties of single isolated smooth muscle cells. Nature. 1977 Feb 10;265(5594):553–556. doi: 10.1038/265553a0. [DOI] [PubMed] [Google Scholar]
  7. Gordon A. M., Huxley A. F., Julian F. J. The variation in isometric tension with sarcomere length in vertebrate muscle fibres. J Physiol. 1966 May;184(1):170–192. doi: 10.1113/jphysiol.1966.sp007909. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gordon A. R., Siegman M. J. Mechanical properties of smooth muscle. I. Length-tension and force-velocity relations. Am J Physiol. 1971 Nov;221(5):1243–1249. doi: 10.1152/ajplegacy.1971.221.5.1243. [DOI] [PubMed] [Google Scholar]
  9. Halpern W., Mulvany M. J., Warshaw D. M. Mechanical properties of smooth muscle cells in the walls of arterial resistance vessels. J Physiol. 1978 Feb;275:85–101. doi: 10.1113/jphysiol.1978.sp012179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Herlihy J. T., Murphy R. A. Length-tension relationship of smooth muscle of the hog carotid artery. Circ Res. 1973 Sep;33(3):275–283. doi: 10.1161/01.res.33.3.275. [DOI] [PubMed] [Google Scholar]
  11. Julian F. J., Sollins M. R., Moss R. L. Sarcomere length non-uniformity in relation to tetanic responses of stretched skeletal muscle fibres. Proc R Soc Lond B Biol Sci. 1978 Jan 24;200(1138):109–116. doi: 10.1098/rspb.1978.0009. [DOI] [PubMed] [Google Scholar]
  12. Lowy J., Mulvany M. J. Mechanical properties of guinea pig taenia coli muscles. Acta Physiol Scand. 1973 May;88(1):123–136. doi: 10.1111/j.1748-1716.1973.tb05439.x. [DOI] [PubMed] [Google Scholar]
  13. Lowy J., Vibert P. J., Haselgrove J. C., Poulsen F. R. The structure of the myosin elements in vertebrate smooth muscles. Philos Trans R Soc Lond B Biol Sci. 1973 Mar 15;265(867):191–196. doi: 10.1098/rstb.1973.0022. [DOI] [PubMed] [Google Scholar]
  14. Mulvany M. J., Halpern W. Contractile properties of small arterial resistance vessels in spontaneously hypertensive and normotensive rats. Circ Res. 1977 Jul;41(1):19–26. doi: 10.1161/01.res.41.1.19. [DOI] [PubMed] [Google Scholar]
  15. Mulvany M. J., Halpern W. Mechanical properties of vascular smooth muscle cells in situ. Nature. 1976 Apr 15;260(5552):617–619. doi: 10.1038/260617a0. [DOI] [PubMed] [Google Scholar]
  16. Mulvany M. J., Hansen O. K., Aalkjaer C. Direct evidence that the greater contractility of resistance vessels in spontaneously hypertensive rats is associated with a narrowed lumen, a thickened media, and an increased number of smooth muscle cell layers. Circ Res. 1978 Dec;43(6):854–864. doi: 10.1161/01.res.43.6.854. [DOI] [PubMed] [Google Scholar]
  17. Paul R. J., Peterson J. W. Relation between length, isometric force, and O2 consumption rate in vascular smooth muscle. Am J Physiol. 1975 Mar;228(3):915–922. doi: 10.1152/ajplegacy.1975.228.3.915. [DOI] [PubMed] [Google Scholar]
  18. Peterson J. W., Paul R. J. Effects of initial length and active shortening on vascular smooth muscle contractility. Am J Physiol. 1974 Nov;227(5):1019–1024. doi: 10.1152/ajplegacy.1974.227.5.1019. [DOI] [PubMed] [Google Scholar]
  19. Rüdel R., Taylor S. R. Striated muscle fibers: facilitation of contraction at short lengths by caffeine. Science. 1971 Apr 23;172(3981):387–389. doi: 10.1126/science.172.3981.387. [DOI] [PubMed] [Google Scholar]
  20. Small J. V. Studies on isolated smooth muscle cells: The contractile apparatus. J Cell Sci. 1977 Apr;24:327–349. doi: 10.1242/jcs.24.1.327. [DOI] [PubMed] [Google Scholar]
  21. Speden R. N. The effect of initial strip length on the noradrenaline-induced contraction of arterial strips. J Physiol. 1960 Nov;154(1):15–25. doi: 10.1113/jphysiol.1960.sp006561. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Uvelius B. Isometric and isotonic length-tension relations and variaitonsin cell length in longitudinal smooth muscel from rabbit urinary bladder. Acta Physiol Scand. 1976 Mar;97(1):1–12. doi: 10.1111/j.1748-1716.1976.tb10230.x. [DOI] [PubMed] [Google Scholar]
  23. Winton F. R. The influence of length on the responses of unstriated muscle to electrical and chemical stimulation, and stretching. J Physiol. 1926 Jun 22;61(3):368–382. doi: 10.1113/jphysiol.1926.sp002300. [DOI] [PMC free article] [PubMed] [Google Scholar]

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