Skip to main content
The Journal of Biophysical and Biochemical Cytology logoLink to The Journal of Biophysical and Biochemical Cytology
. 1959 Aug 1;6(1):53–56. doi: 10.1083/jcb.6.1.53

The Isolation of Nucleoli from Ungerminated Pea Embryos

F B Johnston 1, G Setterfield 1, Herbert Stern 1
PMCID: PMC2229752  PMID: 13673046

Abstract

A method is described for the isolation of nuclei and nucleoli from ungerminated pea embryos. Electron micrographs of the nucleolar preparation indicate a high degree of purity but only a partial preservation of composition, because of a loss of material from the nucleoli which occurs during the isolation procedure.

Full Text

The Full Text of this article is available as a PDF (610.3 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. JOHNSTON F. B., STERN H. Mass isolation of viable wheat embryos. Nature. 1957 Jan 19;179(4551):160–161. doi: 10.1038/179160b0. [DOI] [PubMed] [Google Scholar]
  2. Johnston F. B., Nasatir M., Stern H. The Isolation of Nuclei and Oxidative Particles from Viable Wheat Embryos. Plant Physiol. 1957 Mar;32(2):124–129. doi: 10.1104/pp.32.2.124. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. LAFONTAINE J. G. A particulate component found in nucleoli of Allium cepa and Vicia faba. J Biophys Biochem Cytol. 1958 Mar 25;4(2):229–230. doi: 10.1083/jcb.4.2.229. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from The Journal of Biophysical and Biochemical Cytology are provided here courtesy of The Rockefeller University Press

RESOURCES