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. 1969 Dec;64(4):1356–1361. doi: 10.1073/pnas.64.4.1356

TOLERANCE OF RAT SKIN GRAFTS IN ADULT MICE

E M Lance 1,*, R H Levey 1,†, P B Medawar 1, M Ruszkiewicz 1,‡
PMCID: PMC223292  PMID: 4393916

Abstract

Adult CBA mice may be made tolerant of August rat tail skin grafts by short intensive treatments with antilymphocyte serum followed by massive (∼100 × 106 cells) injections of August rat lymphoid cells. Prolonged tolerance can be achieved only in thymectomized mice, and is accompanied by lymphoid cell chimerism and the manufacture of rat protein. The rat lymphoid cell donors must be treated beforehand with antirat ALS to avoid complications associated with graft versus host reactions.

Rejection of rat skin grafts is accompanied by the formation of high titres of antirat hemolysins and lymphocytotoxins, but in “tolerant” mice the rat skin survives in the continual presence of antirat antibodies. Under certain circumstances, however, passive transfusion of high titre mouse antirat serum can precipitate the breakdown of rat skin grafts. It is doubtful if this represents the primary mechanism of rejection. The reaction of normal mice against rat skin is essentially an intensified allograft reaction; it is at all events wholly immunological in character.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ben-Hur N., Solowey A. C., Rapaport F. T. The xenograft rejection phenomenon. I. Response of the mouse to rabbit, guinea pig and rat skin xenografts. Isr J Med Sci. 1969 Jan-Feb;5(1):1–12. [PubMed] [Google Scholar]
  2. Calne R. Y., White H. J., Herbertson B. M., Millard P. R., Davis D. R., Salaman J. R., Samuel J. R. Pig-to-baboon liver xenografts. Lancet. 1968 Jun 1;1(7553):1176–1178. doi: 10.1016/s0140-6736(68)91869-2. [DOI] [PubMed] [Google Scholar]
  3. Floersheim G. L., Ruszkiewicz M. Bone-marrow transplantation after antilymphocytic serum and lethal chemotherapy. Nature. 1969 May 31;222(5196):854–857. doi: 10.1038/222854a0. [DOI] [PubMed] [Google Scholar]
  4. GRABAR P., COURCON J., BARNES D. W., FORD C. E., MICKLEM H. S. Study of the antigenic constituents of sera from mouse/rat chimaeras. Immunology. 1962 Nov;5:673–686. [PMC free article] [PubMed] [Google Scholar]
  5. GRABAR P., COURCON J., ILBERG P. L., LOUTIT J. F., MERRILL J. P. Etude immuno-électrophorétique du sérum de souris irradiées par des doses létales de rayons X et protégées par des cellules de la moelle osseuse de rats. C R Hebd Seances Acad Sci. 1957 Sep 2;245(10):950–952. [PubMed] [Google Scholar]
  6. HASKOVA V., CHUTNA J., HASEK M., HORT J. Destruction of tolerated skin heterografts by means of serum antibodies, and the role of antibodies in graft rejection. Ann N Y Acad Sci. 1962 Oct 24;99:602–605. doi: 10.1111/j.1749-6632.1962.tb45342.x. [DOI] [PubMed] [Google Scholar]
  7. Jooste S. V., Lance E. M., Levey R. H., Medawar P. B., Ruszkiewicz M., Sharman R., Taub R. N. Notes on the preparation and assay of anti-lymphocytic serum for use in mice. Immunology. 1968 Nov;15(5):697–705. [PMC free article] [PubMed] [Google Scholar]
  8. Lance E. M., Ford P. J., Ruszkiewicz M. The use of subcellular fractions to raise anti-lymphocytic serum. Immunology. 1968 Oct;15(4):571–580. [PMC free article] [PubMed] [Google Scholar]
  9. Lance E. M., Medawar P. B. Survival of skin heterografts under treatment with antilymphocytic serum. Lancet. 1968 Jun 1;1(7553):1174–1176. doi: 10.1016/s0140-6736(68)91868-0. [DOI] [PubMed] [Google Scholar]
  10. Lance E. M., Medawar P. Quantitative studies on tissue transplantation immunity. IX. Induction of tolerance with antilymphocytic serum. Proc R Soc Lond B Biol Sci. 1969 Jul 22;173(1033):447–473. doi: 10.1098/rspb.1969.0071. [DOI] [PubMed] [Google Scholar]
  11. Levey R. H., Medawar P. B. Further experiments on the action of antilymphocytic antiserum. Proc Natl Acad Sci U S A. 1967 Aug;58(2):470–477. doi: 10.1073/pnas.58.2.470. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Levey R. H., Medawar P. B. Nature and mode of action of antilymphocytic antiserum. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1130–1137. doi: 10.1073/pnas.56.4.1130. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Monaco A. P., Wood M. L., Gray J. G., Russell P. S. Studies on heterologous anti-lymphocyte serum in mice. II. Effect on the immune response. J Immunol. 1966 Feb;96(2):229–238. [PubMed] [Google Scholar]
  14. Monaco A. P., Wood M. L., Russell P. S. Adult Thymectomy: Effect on Recovery from Immunologic Depression in Mice. Science. 1965 Jul 23;149(3682):432–435. doi: 10.1126/science.149.3682.432. [DOI] [PubMed] [Google Scholar]
  15. SANDERSON A. R. CYTOTOXIC REACTIONS OF MOUSE ISO-ANTISERA: PRELIMINARY CONSIDERATIONS. Br J Exp Pathol. 1964 Aug;45:398–408. [PMC free article] [PubMed] [Google Scholar]
  16. Taub R. N., Lance E. M. Effects of heterologous anti-lymphocyte serum on the distribution of 51-Cr-labelled lymph node cells in mice. Immunology. 1968 Nov;15(5):633–642. [PMC free article] [PubMed] [Google Scholar]
  17. Voisin G. A., Kinsky R. G., Maillard J. Réativité immunitaire et anticorps facilitants chez des animaux tolérants aux homogreffes. Ann Inst Pasteur (Paris) 1968 Nov;115(5):855–879. [PubMed] [Google Scholar]

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