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. 1969 Nov;64(3):962–969. doi: 10.1073/pnas.64.3.962

EFFECT OF THE LAMBDA REPRESSOR ON THE BINDING OF RNA POLYMERASE TO DNA

William S Hayward 1,*, Melvin H Green 1
PMCID: PMC223329  PMID: 5264150

Abstract

Experiments were designed with the aim of understanding the mechanism whereby the lambda repressor inhibits messenger RNA synthesis. Complexes containing lambda DNA and RNA polymerase were isolated from repressed and derepressed lysogens and analyzed for the relative amounts of RNA polymerase per phage genome. The data indicate that the repressor inhibits the binding of RNA polymerase to promoter sites known to be under direct control of the repressor.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baker R. F., Yanofsky C. The periodicity of RNA polymerase initiations: a new regulatory feature of transcription. Proc Natl Acad Sci U S A. 1968 May;60(1):313–320. doi: 10.1073/pnas.60.1.313. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. CAMPBELL A. Sensitive mutants of bacteriophage lambda. Virology. 1961 May;14:22–32. doi: 10.1016/0042-6822(61)90128-3. [DOI] [PubMed] [Google Scholar]
  3. Chesterton C. J., Green M. H. Early-late transcription switch: isolation of a lambda DNA-RNA polymerase complex active in the synthesis of late RNA. Biochem Biophys Res Commun. 1968 Jun 28;31(6):919–924. doi: 10.1016/0006-291x(68)90540-8. [DOI] [PubMed] [Google Scholar]
  4. Cohen S. N., Hurwitz J. Genetic transcription in bacteriophage lambda: studies of lambda m RNA synthesis in vivo. J Mol Biol. 1968 Nov 14;37(3):387–406. doi: 10.1016/0022-2836(68)90110-1. [DOI] [PubMed] [Google Scholar]
  5. Eisen H. A., Fuerst C. R., Siminovitch L., Thomas R., Lambert L., Pereira da Silva L., Jacob F. Genetics and physiology of defective lysogeny in K12 (lambda): studies of early mutants. Virology. 1966 Oct;30(2):224–241. doi: 10.1016/0042-6822(66)90098-5. [DOI] [PubMed] [Google Scholar]
  6. Gilbert W., Müller-Hill B. The lac operator is DNA. Proc Natl Acad Sci U S A. 1967 Dec;58(6):2415–2421. doi: 10.1073/pnas.58.6.2415. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gillespie D., Spiegelman S. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol. 1965 Jul;12(3):829–842. doi: 10.1016/s0022-2836(65)80331-x. [DOI] [PubMed] [Google Scholar]
  8. Green M. H., Gotchel B., Hendershott J., Kennel S. Regulation of bacteriophage lambda DNA replication. Proc Natl Acad Sci U S A. 1967 Dec;58(6):2343–2350. doi: 10.1073/pnas.58.6.2343. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Green M. H. Inactivation of the prophage lambda repressor without induction. J Mol Biol. 1966 Mar;16(1):134–148. doi: 10.1016/s0022-2836(66)80268-1. [DOI] [PubMed] [Google Scholar]
  10. Hayashi M., Spiegelman S., Franklin N. C., Luria S. E. SEPARATION OF THE RNA MESSAGE TRANSCRIBED IN RESPONSE TO A SPECIFIC INDUCER. Proc Natl Acad Sci U S A. 1963 May;49(5):729–736. doi: 10.1073/pnas.49.5.729. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hogness D. S., Doerfler W., Egan J. B., Black L. W. The position and orientation of genes in lambda and lambda dg DNA. Cold Spring Harb Symp Quant Biol. 1966;31:129–138. doi: 10.1101/sqb.1966.031.01.020. [DOI] [PubMed] [Google Scholar]
  12. Howard B. D. Phage lambda mutants deficient in r-II exclusion. Science. 1967 Dec 22;158(3808):1588–1589. doi: 10.1126/science.158.3808.1588. [DOI] [PubMed] [Google Scholar]
  13. Imamoto F. On the initiation of transcription of the tryptophan operon in Escherichia coli. Proc Natl Acad Sci U S A. 1968 May;60(1):305–312. doi: 10.1073/pnas.60.1.305. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. JACOB F., MONOD J. Genetic regulatory mechanisms in the synthesis of proteins. J Mol Biol. 1961 Jun;3:318–356. doi: 10.1016/s0022-2836(61)80072-7. [DOI] [PubMed] [Google Scholar]
  15. KAISER A. D., JACOB F. Recombination between related temperate bacteriophages and the genetic control of immunity and prophage localization. Virology. 1957 Dec;4(3):509–521. doi: 10.1016/0042-6822(57)90083-1. [DOI] [PubMed] [Google Scholar]
  16. KAISER A. D. The production of phage chromosome fragments and their capacity for genetic transfer. J Mol Biol. 1962 Apr;4:275–287. doi: 10.1016/s0022-2836(62)80005-9. [DOI] [PubMed] [Google Scholar]
  17. Kumar S., Bovre K., Guha A., Hradecna Z., Maher V. R., Szybalski W. Orientation and control of transcription in E. coli phage lambda. Nature. 1969 Mar 1;221(5183):823–825. doi: 10.1038/221823a0. [DOI] [PubMed] [Google Scholar]
  18. Lieb M. Studies of heat-inducible lambda bacteriophage. I. Order of genetic sites and properties of mutant prophages. J Mol Biol. 1966 Mar;16(1):149–163. doi: 10.1016/s0022-2836(66)80269-3. [DOI] [PubMed] [Google Scholar]
  19. Malva C., Razzino G., Calef E. A possible role of C3 gene of lambda prophage in the maintenance of the lysogenic state. Virology. 1969 Jun;38(2):358–360. doi: 10.1016/0042-6822(69)90381-x. [DOI] [PubMed] [Google Scholar]
  20. Miller J. H., Ippen K., Scaife J. G., Beckwith J. R. The promoter-operator region of the lac operon of Escherichia coli. J Mol Biol. 1968 Dec;38(3):413–420. doi: 10.1016/0022-2836(68)90395-1. [DOI] [PubMed] [Google Scholar]
  21. Ptashne M., Hopkins N. The operators controlled by the lambda phage repressor. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1282–1287. doi: 10.1073/pnas.60.4.1282. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Ptashne M. Specific binding of the lambda phage repressor to lambda DNA. Nature. 1967 Apr 15;214(5085):232–234. doi: 10.1038/214232a0. [DOI] [PubMed] [Google Scholar]
  23. Riggs A. D., Bourgeois S., Newby R. F., Cohn M. DNA binding of the lac repressor. J Mol Biol. 1968 Jul 14;34(2):365–368. doi: 10.1016/0022-2836(68)90261-1. [DOI] [PubMed] [Google Scholar]
  24. Taylor K., Hradecna Z., Szybalski W. Asymmetric distribution of the transcribing regions on the complementary strands of coliphage lambda DNA. Proc Natl Acad Sci U S A. 1967 Jun;57(6):1618–1625. doi: 10.1073/pnas.57.6.1618. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Umezawa H., Mizuno S., Yamazaki H., Nitta K. Inhibition of DNA-dependent RNA synthesis by rifamycins. J Antibiot (Tokyo) 1968 Mar;21(3):234–236. doi: 10.7164/antibiotics.21.234. [DOI] [PubMed] [Google Scholar]
  26. Wehrli W., Nüesch J., Knüsel F., Staehelin M. Action of rifamycins on RNA polymerase. Biochim Biophys Acta. 1968 Mar 18;157(1):215–217. doi: 10.1016/0005-2787(68)90285-2. [DOI] [PubMed] [Google Scholar]

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