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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1969 Nov;64(3):1042–1048. doi: 10.1073/pnas.64.3.1042

ISOLATION AND PARTIAL CHARACTERIZATION OF A MAJOR INOSITOL-CONTAINING LIPID IN BAKER'S YEAST, MANNOSYL-DIINOSITOL, DIPHOSPHORYL-CERAMIDE*

S Steiner 1,†, Sharron Smith 1,‡, C J Waechter 1,§, Robert L Lester 1
PMCID: PMC223341  PMID: 4313330

Abstract

A polar lipid containing about 20 per cent of the lipid soluble inositol has been purified from cells of Saccharomyces cerevisiae. This compound labeled with 3H-inositol, 32Pi, or 14C glucose was isolated from aqueous pyridine extracts by means of paper chromatography. Unlabeled compound was obtained by a differential solubility scheme. Analytical and degradation studies suggest that this compound can be formulated as a mannosyl-diinositol, diphosphoryl-ceramide. It can be converted by treatment with base or acid into a mannosylmonoinositol phosphoryl-ceramide, a composition which relates it to the basic structure of the phytoglycolipids.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARTLETT G. R. Phosphorus assay in column chromatography. J Biol Chem. 1959 Mar;234(3):466–468. [PubMed] [Google Scholar]
  2. BITTER T., MUIR H. M. A modified uronic acid carbazole reaction. Anal Biochem. 1962 Oct;4:330–334. doi: 10.1016/0003-2697(62)90095-7. [DOI] [PubMed] [Google Scholar]
  3. CARTER H. E., JOHNSON P., WEBER E. J. GLYCOLIPIDS. Annu Rev Biochem. 1965;34:109–142. doi: 10.1146/annurev.bi.34.070165.000545. [DOI] [PubMed] [Google Scholar]
  4. Carter H. E., Kisic A. Countercurrent distribution of inosol lipids of plant seeds. J Lipid Res. 1969 Jul;10(4):356–362. [PubMed] [Google Scholar]
  5. HAWTHORNE J. N. The inositol phospholipids. J Lipid Res. 1960 Jul;1:255–280. [PubMed] [Google Scholar]
  6. Lester R. L., Steiner M. R. The occurrence of diphosphoinositide and triphosphoinositide in Saccharomyces cerevisiae. J Biol Chem. 1968 Sep 25;243(18):4889–4893. [PubMed] [Google Scholar]
  7. MALLOV S., MCKIBBIN J. M., ROBB J. S. The distribution of some of the essential lipides in beef heart muscle and conducting tissue. J Biol Chem. 1953 Apr;201(2):825–838. [PubMed] [Google Scholar]
  8. SCHWEIGER A. [Separation of simple sugars on cellulose lavers]. J Chromatogr. 1962 Nov;9:374–376. doi: 10.1016/s0021-9673(00)80803-1. [DOI] [PubMed] [Google Scholar]
  9. Steiner S., Conti S. F., Lester R. L. Separation and identification of the polar lipids of Chromatium strain D. J Bacteriol. 1969 Apr;98(1):10–15. doi: 10.1128/jb.98.1.10-15.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

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