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. 1969 Oct;64(2):520–527. doi: 10.1073/pnas.64.2.520

COMPLEMENT COMPONENTS REQUIRED FOR VIRUS NEUTRALIZATION BY EARLY IMMUNOGLOBULIN ANTIBODY*

William D Linscott 1, Warren E Levinson 1
PMCID: PMC223375  PMID: 4982356

Abstract

Virus neutralization by early antisera usually requires or is enhanced by the presence of complement. The particular components of the complement system which are needed for neutralization of Newcastle disease virus by early IgM antibodies were studied. It was found in this system that participation of only the first four of the nine complement components (C1, C2, C3, and C4) was necessary and sufficient for neutralization to occur. It was concluded that the role of complement in neutralization was most likely that of contributing bulk in the form of large protein molecules to the virus-antibody complex.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ashe W. K., Mage M., Mage R., Notkins A. L. Neutralization and sensitization of herpes simplex virus with antibody fragments from rabbits of different allotypes. J Immunol. 1968 Sep;101(3):500–504. [PubMed] [Google Scholar]
  2. Borsos T., Rapp H. J. Complement fixation on cell surfaces by 19S and 7S antibodies. Science. 1965 Oct 22;150(3695):505–506. doi: 10.1126/science.150.3695.505. [DOI] [PubMed] [Google Scholar]
  3. Cochrane C. G., Müller-Eberhard H. J. The derivation of two distinct anaphylatoxin activities from the third and fifth components of human complement. J Exp Med. 1968 Feb 1;127(2):371–386. doi: 10.1084/jem.127.2.371. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. DALES S., KAJIOKA R. THE CYCLE OF MULTIPLICATION OF VACCINIA VIRUS IN EARLE'S STRAIN L CELLS. I. UPTAKE AND PENETRATION. Virology. 1964 Nov;24:278–294. doi: 10.1016/0042-6822(64)90167-9. [DOI] [PubMed] [Google Scholar]
  5. EISEN H. N., SISKIND G. W. VARIATIONS IN AFFINITIES OF ANTIBODIES DURING THE IMMUNE RESPONSE. Biochemistry. 1964 Jul;3:996–1008. doi: 10.1021/bi00895a027. [DOI] [PubMed] [Google Scholar]
  6. GOODMAN J. W., DONCH J. J. NEUTRALIZATION OF BACTERIOPHAGE BY INTACT AND DEGRADED RABBIT ANTIBODY. J Immunol. 1964 Jul;93:96–100. [PubMed] [Google Scholar]
  7. Goodman J. W., Donch J. J. Phage-neutralizing activity in light polypeptide chains of rabbit antibody. Immunochemistry. 1965 Dec;2(4):351–357. doi: 10.1016/0019-2791(65)90035-2. [DOI] [PubMed] [Google Scholar]
  8. Hampar B., Mage M. G., Keehn M. A. Heterogeneity in the ability of rabbit 7S and 19S neutralizing antibodies to differentiate between intratypic antigenic variants of Herpes simplex virus. J Immunol. 1968 Aug;101(2):256–261. [PubMed] [Google Scholar]
  9. Hampar B., Notkins A. L., Mage M., Keehn M. A. Heterogeneity in the properties of 7 S and 19S rabbit-neutralizing antibodies to herpes simplex virus. J Immunol. 1968 Mar;100(3):586–593. [PubMed] [Google Scholar]
  10. Heineman H. S. Herpes simplex neutralizing antibody--quantitation of the complement-dependent fraction in different phases of adult human infection. J Immunol. 1967 Jul;99(1):214–222. [PubMed] [Google Scholar]
  11. Huber H., Polley M. J., Linscott W. D., Fudenberg H. H., Müller-Eberhard H. J. Human monocytes: distinct receptor sites for the third component of complement and for immunoglobulin G. Science. 1968 Dec 13;162(3859):1281–1283. doi: 10.1126/science.162.3859.1281. [DOI] [PubMed] [Google Scholar]
  12. Inoue K., Nelson R. A., Jr The isolation and characterization of a ninth component of hemolytic complement, C'3f. J Immunol. 1966 Mar;96(3):386–400. [PubMed] [Google Scholar]
  13. Inoue K., Yonemasu K., Takamizawa A., Amano T. [Studies on the immune bacteriolysis. XIV. Requirement of all nine components of complement for immune bacteriolysis]. Biken J. 1968 Sep;11(3):203–206. [PubMed] [Google Scholar]
  14. Klinman N. R., Long C. A., Karush F. The role of antibody bivalence in the neutralization of bacteriophage. J Immunol. 1967 Dec;99(6):1128–1133. [PubMed] [Google Scholar]
  15. Levinson W., Rubin H. Radiation studies of avian tumor viruses and of Newcastle disease virus. Virology. 1966 Apr;28(4):533–542. doi: 10.1016/0042-6822(66)90238-8. [DOI] [PubMed] [Google Scholar]
  16. Linscott W. D. Complement: purification of the first component from rabbit and guinea pig serum. Immunochemistry. 1968 May;5(3):311–314. doi: 10.1016/0019-2791(68)90075-x. [DOI] [PubMed] [Google Scholar]
  17. Linscott W. D. Immune hemolysis: serial studies of the dissociability of IgG and IgM anti-Forssman antibodies using the C'la fixation and transfer technique. J Immunol. 1969 Apr;102(4):986–992. [PubMed] [Google Scholar]
  18. MUELLER-EBERHARD H. J., LEPOW I. H. C'1 ESTERASE EFFECT ON ACTIVITY AND PHYSICOCHEMICAL PROPERTIES OF THE FOURTH COMPONENT OF COMPLEMENT. J Exp Med. 1965 May 1;121:819–833. doi: 10.1084/jem.121.5.819. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. MUSCHEL L. H., TOUSSAINT A. J. Studies on the bacteriophage-neutralizing activity of serums. II. Comparison of normal and immune phage-neutralizing antibodies. J Immunol. 1962 Jul;89:35–40. [PubMed] [Google Scholar]
  20. Mardiney M. R., Jr, Müller-Eberhard H. J., Feldman J. D. Ultrastructural localization of the third and fourth components of complement on complement-cell complexes. Am J Pathol. 1968 Aug;53(2):253–261. [PMC free article] [PubMed] [Google Scholar]
  21. Müllerèberhard H. J., Dalmasso A. P., Calcott M. A. The reaction mechanism of beta-1C-globulin (C'3) in immune hemolysis. J Exp Med. 1966 Jan 1;123(1):33–54. doi: 10.1084/jem.123.1.33. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. NISHIOKA K., LINSCOTT W. D. COMPONENTS OF GUINEA PIG COMPLEMENT. I. SEPARATION OF A SERUM FRACTION ESSENTIAL FOR IMMUNE HEMOLYSIS AND IMMUNE ADHERENCE. J Exp Med. 1963 Nov 1;118:767–793. doi: 10.1084/jem.118.5.767. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Nelson R. A., Jr A new concept of immunosuppression in hypersensitivity reactions and in transplantation immunity. Surv Ophthalmol. 1966 Aug;11(4):498–505. [PubMed] [Google Scholar]
  24. Notkins A. L., Mage M., Ashe W. K., Mahar S. Neutralization of sensitized lactic dehydrogenase virus by anti-gammglobulin. J Immunol. 1968 Feb;100(2):314–320. [PubMed] [Google Scholar]
  25. Ozaki Y., Tabeyi K. Studies on the neutralization of Japanese encephalitis virus. I. Application of kinetic neutralization to the measurement of the neutralizing potency of antiserum. J Immunol. 1967 Jun;98(6):1218–1223. [PubMed] [Google Scholar]
  26. Paul W., Benacerraf B. Problems encountered in double diffusion analysis in agar of hapten specific immune systems. I. Complement dependent precipitation. J Immunol. 1965 Dec;95(6):1067–1073. [PubMed] [Google Scholar]
  27. RUBIN H., FRANKLIN R. M. On the mechanism of Newcastle disease virus neutralization by immune serum. Virology. 1957 Feb;3(1):84–95. doi: 10.1016/0042-6822(57)90025-9. [DOI] [PubMed] [Google Scholar]
  28. Rosse W. F., Borsos T., Rapp H. J. Cold-reacting antibodies: the enhancement of antibody fixation by the first component of complement (C'1a). J Immunol. 1968 Feb;100(2):259–265. [PubMed] [Google Scholar]
  29. Rother K., Rother U., Müller-Eberhard H. J., Nilsson J. R. Deficiency of the sixth component of complement in rabbits with an inherited complement defect. J Exp Med. 1966 Oct 1;124(4):773–785. doi: 10.1084/jem.124.4.773. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Rowlands D. T., Jr Precipitation and neutralization of bacteriophage f2 by rabbit antibodies. J Immunol. 1967 May;98(5):958–964. [PubMed] [Google Scholar]
  31. SILVERSTEIN S. C., MARCUS P. I. EARLY STAGES OF NEWCASTLE DISEASE VIRUS-HELA CELL INTERACTION: AN ELECTRON MICROSCOPIC STUDY. Virology. 1964 Jul;23:370–380. doi: 10.1016/0042-6822(64)90259-4. [DOI] [PubMed] [Google Scholar]
  32. Shin H. S., Snyderman R., Friedman E., Mellors A., Mayer M. M. Chemotactic and anaphylatoxic fragment cleaved from the fifth component of guinea pig complement. Science. 1968 Oct 18;162(3851):361–363. doi: 10.1126/science.162.3851.361. [DOI] [PubMed] [Google Scholar]
  33. Stroud R. M., Austen K. F., Mayer M. M. Catalysis of C'2 fixation by C'la. Reaction kinetics, competitive inhibition by TAMe, and transferase hypothesis of the enzymatic action of C'la on C'2, one of its natural substrates. Immunochemistry. 1965 Sep;2(3):219–234. [PubMed] [Google Scholar]
  34. TANIGUCHI S., YOSHINO K. STUDIES ON THE NEUTRALIZATION OF HERPES SIMPLEX VIRUS. II. ANALYSIS OF COMPLEMENT AS THE ANTIBODY-POTENTIATING FACTOR. Virology. 1965 May;26:54–60. doi: 10.1016/0042-6822(65)90025-5. [DOI] [PubMed] [Google Scholar]
  35. TOUSSAINT A. J., MUSCHEL L. H. Studies on the bacteriophageneutralizing activity of serums. I. An assay procedure for normal antibody and complement. J Immunol. 1962 Jul;89:27–34. [PubMed] [Google Scholar]
  36. Ward P. A., Cochrane C. G., Muller-Eberhard H. J. Further studies on the chemotactic factor of complement and its formation in vivo. Immunology. 1966 Aug;11(2):141–153. [PMC free article] [PubMed] [Google Scholar]
  37. YOSHINO K., TANIGUCHI S. STUDIES ON THE NEUTRALIZATION OF HERPES SIMPLEX VIRUS. 3. MECHANISM OF THE ANTIBODY-POTENTIATING ACTION OF COMPLEMENT. Virology. 1965 May;26:61–72. doi: 10.1016/0042-6822(65)90026-7. [DOI] [PubMed] [Google Scholar]
  38. YOSHINO K., TANIGUCHI S. STUDIES ON THE NEUTRALIZATION OF HERPES SIMPLEX VIRUS. I. APPEARANCE OF NEUTRALIZING ANTIBODIES HAVING DIFFERENT GRADES OF COMPLEMENT REQUIREMENT. Virology. 1965 May;26:44–53. doi: 10.1016/0042-6822(65)90024-3. [DOI] [PubMed] [Google Scholar]

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