Abstract
The trypanocidal drug ethidium bromide induced abnormal circular DNA molecules with high frequency in the kinetoplast of Trypanosoma cruzi. These molecules are circular dimers, trimers, or higher circular forms with a contour length which is a multiple of the monomer length usually found in the kinetoplastic DNA of normal trypanosomes. These abnormal molecules represent more than 30 per cent of the kinetoplastic DNA, while in normal kinetoplastic DNA we only observed three circular dimers out of several thousand molecules measured.
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- Clayton D. A., Smith C. A., Jordan J. M., Teplitz M., Vinograd J. Occurrence of complex mitochondrial DNA in normal tissues. Nature. 1968 Dec 7;220(5171):976–979. doi: 10.1038/220976a0. [DOI] [PubMed] [Google Scholar]
- Clayton D. A., Vinograd J. Circular dimer and catenate forms of mitochondrial DNA in human leukaemic leucocytes. Nature. 1967 Nov 18;216(5116):652–657. doi: 10.1038/216652a0. [DOI] [PubMed] [Google Scholar]
- Delain E., Riou G. Ultrastructure des altérations du DNA du kinétoplaste de Trypanosoma cruzi traité par le bromure d'éthidium. C R Acad Sci Hebd Seances Acad Sci D. 1969 Mar 3;268(9):1327–1330. [PubMed] [Google Scholar]
- Delain E., Riou G. Ultrastructure du DNA du kinétoplaste de Trypanosoma cruzi cultivé in vitro. C R Acad Sci Hebd Seances Acad Sci D. 1969 Feb 24;268(8):1225–1227. [PubMed] [Google Scholar]
- Freifelder D., Kleinschmidt A. K. Single-strand breaks in duplex DNA of coliphage T7 as demonstrated by electron microscopy. J Mol Biol. 1965 Nov;14(1):271–278. doi: 10.1016/s0022-2836(65)80246-7. [DOI] [PubMed] [Google Scholar]
- Goebel W., Helinski D. R. Generation of higher multiple circular DNA forms in bacteria. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1406–1413. doi: 10.1073/pnas.61.4.1406. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hudson B., Vinograd J. Catenated circular DNA molecules in HeLa cell mitochondria. Nature. 1967 Nov 18;216(5116):647–652. doi: 10.1038/216647a0. [DOI] [PubMed] [Google Scholar]
- Kiger J. A., Jr, Sinsheimer R. L. Vegetative lambda DNA. IV. Fractionation of replicating lambda DNA on benzoylated-naphthoylated DEAE cellulose. J Mol Biol. 1969 Mar 28;40(3):467–490. doi: 10.1016/0022-2836(69)90166-1. [DOI] [PubMed] [Google Scholar]
- LePecq J. B., Paoletti C. A fluorescent complex between ethidium bromide and nucleic acids. Physical-chemical characterization. J Mol Biol. 1967 Jul 14;27(1):87–106. doi: 10.1016/0022-2836(67)90353-1. [DOI] [PubMed] [Google Scholar]
- Radloff R., Bauer W., Vinograd J. A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells. Proc Natl Acad Sci U S A. 1967 May;57(5):1514–1521. doi: 10.1073/pnas.57.5.1514. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Riou G., Delain E. Electron microscopy of the circular kinetoplastic DNA from Trypanosoma cruzi: occurrence of catenated forms. Proc Natl Acad Sci U S A. 1969 Jan;62(1):210–217. doi: 10.1073/pnas.62.1.210. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Riou G. Induction d'une "dyskinétoplastie" par le bromure d'ethidium clez Trypanosoma CRUZI CULTIVé in vitro. C R Acad Sci Hebd Seances Acad Sci D. 1967 Dec 18;265(25):2004–2007. [PubMed] [Google Scholar]
- Riou G., Paoletti C. Preparation and properties of nuclear and satellite deoxyribonucleic acid of Trypanosoma cruzi. J Mol Biol. 1967 Sep 14;28(2):377–382. doi: 10.1016/s0022-2836(67)80017-2. [DOI] [PubMed] [Google Scholar]