Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1969 Oct;64(2):642–649. doi: 10.1073/pnas.64.2.642

RIBOSOMAL RNA GENES IN BACTERIA: EVIDENCE FOR THE NATURE OF THE PHYSICAL LINKAGE BETWEEN 16S AND 23S RNA GENES Bacillus subtilis*

W Colli 1, M Oishi 1
PMCID: PMC223392  PMID: 4982359

Abstract

The nature of the arrangement of 16S and 23S ribosomal RNA genes on the Bacillus subtilis chromosome was studied by means of a Cs2SO4 density gradient centrifugation technique after complexing DNA-(ribosomal) RNA hybrids with mercuric ions. It was observed that the same fragments of single-stranded DNA with an average molecular weight of 1.9 × 106 which hybridize with 23S RNA also hybridize with 16S RNA. This indicates that genes for both these species of ribosomal RNA are physically linked on such DNA fragments. Some evidence suggests that the two types of genes are interspersed with each other, perhaps in sets, each of which contains a 16S and a 23S ribosomal RNA gene.

Full text

PDF
642

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bernardi G. Chromatography of nucleic acids on hydroxyapatite. Nature. 1965 May 22;206(4986):779–783. doi: 10.1038/206779a0. [DOI] [PubMed] [Google Scholar]
  2. Brown D. D., Weber C. S. Gene linkage by RNA-DNA hybridization. II. Arrangement of the redundant gene sequences for 28 s and 18 s ribosomal RNA. J Mol Biol. 1968 Jun 28;34(3):681–697. doi: 10.1016/0022-2836(68)90189-7. [DOI] [PubMed] [Google Scholar]
  3. Dubnau D., Smith I., Marmur J. Gene conservation in Bacillus species. II. The location of genes concerned with the synthesis of ribosomal components and soluble RNA. Proc Natl Acad Sci U S A. 1965 Sep;54(3):724–730. doi: 10.1073/pnas.54.3.724. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dubnau D., Smith I., Morell P., Marmur J. Gene conservation in Bacillus species. I. Conserved genetic and nucleic acid base sequence homologies. Proc Natl Acad Sci U S A. 1965 Aug;54(2):491–498. doi: 10.1073/pnas.54.2.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. EIGNER J., BOEDTKER H., MICHAELS G. The thermal degradation of nucleic acids. Biochim Biophys Acta. 1961 Jul 22;51:165–168. doi: 10.1016/0006-3002(61)91028-9. [DOI] [PubMed] [Google Scholar]
  6. GEORGIEV G. P., SAMARINA O. P., LERMAN M. I., SMIRNOV M. N., SEVERTZOV A. N. BIOSYNTHESIS OF MESSENGER AND RIBOSOMAL RIBONUCLEIC ACIDS IN THE NUCLEOLOCHROMOSOMAL APPARATUS OF ANIMAL CELLS. Nature. 1963 Dec 28;200:1291–1294. doi: 10.1038/2001291a0. [DOI] [PubMed] [Google Scholar]
  7. MANDELL J. D., HERSHEY A. D. A fractionating column for analysis of nucleic acids. Anal Biochem. 1960 Jun;1:66–77. doi: 10.1016/0003-2697(60)90020-8. [DOI] [PubMed] [Google Scholar]
  8. MIYAZAWA Y., THOMAS C. A., Jr NUCLEOTIDE COMPOSITION OF SHORT SEGMENTS OF DNA MOLECULES. J Mol Biol. 1965 Feb;11:223–237. doi: 10.1016/s0022-2836(65)80053-5. [DOI] [PubMed] [Google Scholar]
  9. OISHI M., YOSHIKAWA H., SUEOKA N. SYNCHRONOUS AND DICHOTOMOUS REPLICATIONS OF THE BACILLUS SUBTILIS CHROMOSOME DURING SPORE GERMINATION. Nature. 1964 Dec 12;204:1069–1073. doi: 10.1038/2041069a0. [DOI] [PubMed] [Google Scholar]
  10. OPARA-KUBINSKA Z., KUBINSKI H., SZYBALSKI W. INTERACTION BETWEEN DENATURED DNA, POLYRIBONUCLEOTIDES, AND RIBOSOMAL RNA: ATTEMPTS AT PREPARATIVE SEPARATION OF THE COMPLEMENTARY DNA STRANDS. Proc Natl Acad Sci U S A. 1964 Oct;52:923–930. doi: 10.1073/pnas.52.4.923. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Oishi M., Oishi A., Sueoka N. Location of genetic loci of soluble RNA on Bacillus subtilis chromosome. Proc Natl Acad Sci U S A. 1966 May;55(5):1095–1103. doi: 10.1073/pnas.55.5.1095. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Oishi M., Sueoka N. Location of genetic loci of ribosomal RNA on Bacillus subtilis chromosome. Proc Natl Acad Sci U S A. 1965 Aug;54(2):483–491. doi: 10.1073/pnas.54.2.483. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Oishi M. The transcribing strands of bacillus subtilis DNA for ribosomal and transfer RNA. Proc Natl Acad Sci U S A. 1969 Jan;62(1):256–262. doi: 10.1073/pnas.62.1.256. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Perry R. P. THE CELLULAR SITES OF SYNTHESIS OF RIBOSOMAL AND 4S RNA. Proc Natl Acad Sci U S A. 1962 Dec;48(12):2179–2186. doi: 10.1073/pnas.48.12.2179. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Quagliarotti G., Ritossa F. M. On the arrangement of genes for 28 s and 18 s ribosomal RNA's in Drosophila melanogaster. J Mol Biol. 1968 Aug 28;36(1):57–69. doi: 10.1016/0022-2836(68)90219-2. [DOI] [PubMed] [Google Scholar]
  16. Roger M., Beckmann C. O., Hotchkiss R. D. Separation of native and denatured fractions from partially denatured pneumococcal DNA. J Mol Biol. 1966 Jun;18(1):156–173. doi: 10.1016/s0022-2836(66)80083-9. [DOI] [PubMed] [Google Scholar]
  17. Roger M. Chromatographic resolution of complementary strands of denatured Pneumococcal DNA. Proc Natl Acad Sci U S A. 1968 Jan;59(1):200–207. doi: 10.1073/pnas.59.1.200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rudner R., Karkas J. D., Chargaff E. Separation of B. subtilis DNA into complementary strands, I. Biological properties. Proc Natl Acad Sci U S A. 1968 Jun;60(2):630–635. doi: 10.1073/pnas.60.2.630. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. SAITO H., MIURA K. I. PREPARATION OF TRANSFORMING DEOXYRIBONUCLEIC ACID BY PHENOL TREATMENT. Biochim Biophys Acta. 1963 Aug 20;72:619–629. [PubMed] [Google Scholar]
  20. SCHERRER K., LATHAM H., DARNELL J. E. Demonstration of an unstable RNA and of a precursor to ribosomal RNA in HeLa cells. Proc Natl Acad Sci U S A. 1963 Feb 15;49:240–248. doi: 10.1073/pnas.49.2.240. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. STUDIER F. W. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA. J Mol Biol. 1965 Feb;11:373–390. doi: 10.1016/s0022-2836(65)80064-x. [DOI] [PubMed] [Google Scholar]
  22. Smith I., Dubnau D., Morrell P., Marmur J. Chromosomal location of DNA base sequences complementary to transfer RNA and to 5 s, 16 s and 23 s ribosomal RNA in Bacillus subtilis. J Mol Biol. 1968 Apr 14;33(1):123–140. doi: 10.1016/0022-2836(68)90285-4. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES