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. 1969 Oct;64(2):693–700. doi: 10.1073/pnas.64.2.693

RIBONUCLEASE V OF ESCHERICHIA COLI, I. DEPENDENCE ON RIBOSOMES AND TRANSLOCATION*

Michihiko Kuwano 1, Catherine Ning Kwan 1, David Apirion 1, David Schlessinger 1
PMCID: PMC223400  PMID: 4901707

Abstract

A new RNase activity, tentatively named RNase V, was found in cell-free extracts of E. coli. This activity requires ribosomes, G and T factors, tRNA, K+ or NH4+, Mg2+, GTP, and a sulfhydryl compound to degrade poly U, poly A, T4 phage mRNA, or E. coli mRNA. RNase V is specific for mRNA; it does not attack ribosomal RNA. It is inhibited by antibiotics that decrease breakdown of mRNA in vivo, such as chloramphenicol and streptomycin, and by such agents as 5′-β, γ-methylene-guanosine triphosphate, and fusidic acid, which inhibit ribosome-dependent GTPase and translocation of ribosomes along mRNA. The evidence suggests that RNase V is either an integral part of the ribosome or is tightly associated with it, and that it selectively degrades mRNA in intact cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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