Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1969 Aug;63(4):1144–1150. doi: 10.1073/pnas.63.4.1144

BIOCHEMICAL EVIDENCE FOR INDUCTION BY POLYOMA VIRUS OF REPLICATION OF THE CHROMOSOMES OF MOUSE KIDNEY CELLS

R Hancock 1, R Weil 1
PMCID: PMC223441  PMID: 4311251

Abstract

It was shown earlier that in cultures of contact-inhibited mouse kidney cells infection with polyoma virus induces replication of the chromosomal DNA. In this paper we present evidence, based on analyses of isolated chromatin, that, parallel with and as a consequence of the virus-induced synthesis of cellular DNA, the chromosomal proteins are synthesized in the proportions characteristic of normal chromosome replication. These results are compatible with the hypothesis that polyoma virus activates (or derepresses) a regulatory system of the host cell which controls initiation of chromosome replication.

Full text

PDF
1144

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BURTON K. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid. Biochem J. 1956 Feb;62(2):315–323. doi: 10.1042/bj0620315. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bonner J., Dahmus M. E., Fambrough D., Huang R. C., Marushige K., Tuan D. Y. The Biology of Isolated Chromatin: Chromosomes, biologically active in the test tube, provide a powerful tool for the study of gene action. Science. 1968 Jan 5;159(3810):47–56. doi: 10.1126/science.159.3810.47. [DOI] [PubMed] [Google Scholar]
  3. Borun T. W., Scharff M. D., Robbins E. Rapidly labeled, polyribosome-associated RNA having the properties of histone messenger. Proc Natl Acad Sci U S A. 1967 Nov;58(5):1977–1983. doi: 10.1073/pnas.58.5.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chalkley G. R., Maurer H. R. Turnover of template-bound histone. Proc Natl Acad Sci U S A. 1965 Aug;54(2):498–505. doi: 10.1073/pnas.54.2.498. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. DINGMAN C. W., SPORN M. B. STUDIES ON CHROMATIN. I. ISOLATION AND CHARACTERIZATION OF NUCLEAR COMPLEXES OF DEOXYRIBONUCLEIC ACID, RIBONUCLEIC ACID, AND PROTEIN FROM EMBRYONIC AND ADULT TISSUES OF THE CHICKEN. J Biol Chem. 1964 Oct;239:3483–3492. [PubMed] [Google Scholar]
  6. DULBECCO R., HARTWELL L. H., VOGT M. INDUCTION OF CELLULAR DNA SYNTHESIS BY POLYOMA VIRUS. Proc Natl Acad Sci U S A. 1965 Feb;53:403–410. doi: 10.1073/pnas.53.2.403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gershon D., Hausen P., Sachs L., Winocour E. On the mechanism of polyoma virus-induced synthesis of cellular DNA. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1584–1592. doi: 10.1073/pnas.54.6.1584. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gurley L. R., Hardin J. M. The metabolism of histone fractions. I. Synthesis of histone fractions during the life cycle of mammalian cells. Arch Biochem Biophys. 1968 Nov;128(2):285–292. doi: 10.1016/0003-9861(68)90034-9. [DOI] [PubMed] [Google Scholar]
  9. Hancock R. Conservation of histones in chromatin during growth and mitosis in vitro. J Mol Biol. 1969 Mar 28;40(3):457–466. doi: 10.1016/0022-2836(69)90165-x. [DOI] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Loening U. E. The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochem J. 1967 Jan;102(1):251–257. doi: 10.1042/bj1020251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Moss B., Ingram V. M. The repression and induction by thyroxin of hemoglobin synthesis during amphibian metamorphosis. Proc Natl Acad Sci U S A. 1965 Sep;54(3):967–974. doi: 10.1073/pnas.54.3.967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. REISFELD R. A., LEWIS U. J., WILLIAMS D. E. Disk electrophoresis of basic proteins and peptides on polyacrylamide gels. Nature. 1962 Jul 21;195:281–283. doi: 10.1038/195281a0. [DOI] [PubMed] [Google Scholar]
  14. Robbins E., Borun T. W. The cytoplasmic synthesis of histones in hela cells and its temporal relationship to DNA replication. Proc Natl Acad Sci U S A. 1967 Feb;57(2):409–416. doi: 10.1073/pnas.57.2.409. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Takai S., Borun T. W., Muchmore J., Lieberman I. Concurrent synthesis of histone and deoxyribonucleic acid in liver after partial hepatectomy. Nature. 1968 Aug 24;219(5156):860–861. doi: 10.1038/219860a0. [DOI] [PubMed] [Google Scholar]
  16. WINOCOUR E. Purification of polyoma virus. Virology. 1963 Feb;19:158–168. doi: 10.1016/0042-6822(63)90005-9. [DOI] [PubMed] [Google Scholar]
  17. Weil R., Michel M. R., Ruschmann G. K. Induction of cellular DNA synthesis by polyoma virus. Proc Natl Acad Sci U S A. 1965 Jun;53(6):1468–1475. doi: 10.1073/pnas.53.6.1468. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES