Abstract
The his4 region of yeast contains the information necessary for the catalysis of three steps in the histidine biosynthetic pathway. The three activities specified by this region remain physically associated during gel filtration and ultracentrifugation and after extensive purification. Nonsense mutations in the “operator distal” his4C region lower the molecular weight of the two proximal activities. The proximal activities can, therefore, function without the his4C portion of the protein(s). These observations are compatible with the following three possibilities. The his4 region codes for: (1) an aggregate of three proteins forming a multiprotein complex; (2) a single protein with three catalytic activities; and (3) a single protein which is cleaved to form three proteins which remain associated.
Full text
PDF







Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bauerle R. H., Margolin P. A multifunctional enzyme complex in the tryptophan pathway of Salmonella typhimurium: comparison of polarity and pseudopolarity mutations. Cold Spring Harb Symp Quant Biol. 1966;31:203–214. doi: 10.1101/sqb.1966.031.01.028. [DOI] [PubMed] [Google Scholar]
- Fink G. R. A cluster of genes controlling three enzymes in histidine biosynthesis in Saccharomyces cerevisiae. Genetics. 1966 Mar;53(3):445–459. doi: 10.1093/genetics/53.3.445. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GAREN A., LEVINTHAL C. A fine-structure genetic and chemical study of the enzyme alkaline phosphatase of E. coli. I. Purification and characterization of alkaline phosphatase. Biochim Biophys Acta. 1960 Mar 11;38:470–483. doi: 10.1016/0006-3002(60)91282-8. [DOI] [PubMed] [Google Scholar]
- Giles N. H., Case M. E., Partridge C. W., Ahmed S. I. A gene cluster in Nuerospora crassa coding for an aggregate of five aromatic synthetic enzymes. Proc Natl Acad Sci U S A. 1967 Oct;58(4):1453–1460. doi: 10.1073/pnas.58.4.1453. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gilmore R. A., Stewart J. W., Sherman F. Amino acid replacements resulting from super-suppression of a nonsense mutant of yeast. Biochim Biophys Acta. 1968 Jun 18;161(1):270–272. doi: 10.1016/0005-2787(68)90322-5. [DOI] [PubMed] [Google Scholar]
- Gilmore R. A. Super-suppressors in Saccharomyces cerevisiae. Genetics. 1967 Aug;56(4):641–658. doi: 10.1093/genetics/56.4.641. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hawthorne D C, Mortimer R K. Chromosome Mapping in Saccharomyces: Centromere-Linked Genes. Genetics. 1960 Aug;45(8):1085–1110. doi: 10.1093/genetics/45.8.1085. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hawthorne D. C., Mortimer R. K. Genetic mapping of nonsense suppressors in yeast. Genetics. 1968 Dec;60(4):735–742. doi: 10.1093/genetics/60.4.735. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jacobson M. F., Baltimore D. Polypeptide cleavages in the formation of poliovirus proteins. Proc Natl Acad Sci U S A. 1968 Sep;61(1):77–84. doi: 10.1073/pnas.61.1.77. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MANNEY T. R., MORTIMER R. K. ALLELIC MAPPING IN YEAST BY X-RAY-INDUCED MITOTIC REVERSION. Science. 1964 Feb 7;143(3606):581–583. doi: 10.1126/science.143.3606.581. [DOI] [PubMed] [Google Scholar]
- Manney T. R. Evidence for chain termination by super-suppressible mutants in yeast. Genetics. 1968 Dec;60(4):719–733. doi: 10.1093/genetics/60.4.719. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ullmann A., Jacob F., Monod J. Characterization by in vitro complementation of a peptide corresponding to an operator-proximal segment of the beta-galactosidase structural gene of Escherichia coli. J Mol Biol. 1967 Mar 14;24(2):339–343. doi: 10.1016/0022-2836(67)90341-5. [DOI] [PubMed] [Google Scholar]
- Vallee B. L., Hoch F. L. ZINC, A COMPONENT OF YEAST ALCOHOL DEHYDROGENASE. Proc Natl Acad Sci U S A. 1955 Jun 15;41(6):327–338. doi: 10.1073/pnas.41.6.327. [DOI] [PMC free article] [PubMed] [Google Scholar]