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. 1969 Aug;63(4):1343–1350. doi: 10.1073/pnas.63.4.1343

MECHANISM OF DNA CHAIN GROWTH, III. EQUAL ANNEALING OF T4 NASCENT SHORT DNA CHAINS WITH THE SEPARATED COMPLEMENTARY STRANDS OF THE PHAGE DNA*

Kazunori Sugimoto 1, Tuneko Okazaki 1, Yasuo Imae 1, Reiji Okazaki 1
PMCID: PMC223470  PMID: 5260937

Abstract

Nascent short DNA chains isolated from T4-infected E. coli under a variety of conditions anneal equally to the separated complementary phage DNA strands. The samples examined include: pulse-labeled short chains isolated by alkaline sucrose gradient sedimentation from the T4D (wild type)-infected cells in both the early and late stages of phage DNA synthesis; nascent chains accumulated during ligase inhibition of T4 ts B20-infected cells; and the single-stranded nascent short chains isolated from T4D-infected cells by mild procedures involving no denaturation treatment. The results are consistent with the hypothesis that both strands of DNA are synthesized discontinuously.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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