Abstract
The free and membrane-bound ribosomes of hepatic cells were isolated and used to program protein synthesis in cell-free extracts. Immuno-electrophoretic and radioautographic analysis of the products showed that the membrane-bound ribosomes synthesized serum proteins, whereas ribosomes free of endoplasmic reticulum synthesized specific nonserum liver proteins. After synthesis, the serum proteins remained associated with membrane vesicles, whereas the other liver proteins were found free of cytoplasmic organelles.
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- AMES B. N., DUBIN D. T. The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid. J Biol Chem. 1960 Mar;235:769–775. [PubMed] [Google Scholar]
- Andrews T. M., Tata J. R. Difference in vectorial release of nascent protein from membrane-bound ribosomes of secretory and non-secretory tissues. Biochem Biophys Res Commun. 1968 Sep 30;32(6):1050–1056. doi: 10.1016/0006-291x(68)90136-8. [DOI] [PubMed] [Google Scholar]
- Bishop J. O. Initiation of haemoglobin polypeptide chains. Biochim Biophys Acta. 1966 Apr 18;119(1):130–145. doi: 10.1016/0005-2787(66)90045-1. [DOI] [PubMed] [Google Scholar]
- Bishop J. O. Persistence of native ribosomal subunits during reticulocyte maturation. J Mol Biol. 1966 May;17(1):285–288. doi: 10.1016/s0022-2836(66)80109-2. [DOI] [PubMed] [Google Scholar]
- Blobel G., Potter V. R. Relation of ribonuclease and ribonuclease inhibitor to the isolation of polysomes from rat liver. Proc Natl Acad Sci U S A. 1966 May;55(5):1283–1288. doi: 10.1073/pnas.55.5.1283. [DOI] [PMC free article] [PubMed] [Google Scholar]
- CAMPBELL P. N., GREENGARD O., KERNOT B. A. Studies on the synthesis o serum albumin by the isolated microsome fraction from rat liver. Biochem J. 1960 Jan;74:107–117. doi: 10.1042/bj0740107. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FOLCH J., LEES M., SLOANE STANLEY G. H. A simple method for the isolation and purification of total lipides from animal tissues. J Biol Chem. 1957 May;226(1):497–509. [PubMed] [Google Scholar]
- GANOZA M. C., WILLIAMS C. A., LIPMANN F. SYNTHESIS OF SERUM PROTEINS BY A CELL-FREE SYSTEM FROM RAT LIVER. Proc Natl Acad Sci U S A. 1965 Mar;53:619–622. doi: 10.1073/pnas.53.3.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
- GIERER A., SCHRAMM G. Infectivity of ribonucleic acid from tobacco mosaic virus. Nature. 1956 Apr 14;177(4511):702–703. doi: 10.1038/177702a0. [DOI] [PubMed] [Google Scholar]
- Hicks S. J., Drysdale J. W., Munro H. N. Preferential synthesis of ferritin and albumin by different populations of liver polysomes. Science. 1969 May 2;164(3879):584–585. doi: 10.1126/science.164.3879.584. [DOI] [PubMed] [Google Scholar]
- Jamieson J. D., Palade G. E. Intracellular transport of secretory proteins in the pancreatic exocrine cell. 3. Dissociation of intracellular transport from protein synthesis. J Cell Biol. 1968 Dec;39(3):580–588. doi: 10.1083/jcb.39.3.580. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Joklik W. K., Becker Y. Studies on the genesis of polyribosomes. II. The association of nascent messenger RNA with the 40 S subribosomal particle. J Mol Biol. 1965 Sep;13(2):511–520. doi: 10.1016/s0022-2836(65)80113-9. [DOI] [PubMed] [Google Scholar]
- Kaempfer R. O., Meselson M., Raskas H. J. Cyclic dissociation into stable subunits and re-formation of ribosomes during bacterial growth. J Mol Biol. 1968 Jan 28;31(2):277–289. doi: 10.1016/0022-2836(68)90444-0. [DOI] [PubMed] [Google Scholar]
- Loeb J. N., Howell R., Tomkins G. M. Free and membrane-bound ribosomes in rat liver. J Biol Chem. 1967 May 10;242(9):2069–2074. [PubMed] [Google Scholar]
- Mangiarotti G., Schlessinger D. Polyribosome metabolism in Escherichia coli. I. Extraction of polyribosomes and ribosomal subunits from fragile, growing Escherichia coli. J Mol Biol. 1966 Sep;20(1):123–143. doi: 10.1016/0022-2836(66)90122-7. [DOI] [PubMed] [Google Scholar]
- Murty C. N., Hallinan T. RNA turnover in free and endoplasmic reticulum-bound ribosomes from rat liver. Biochim Biophys Acta. 1968 Apr 22;157(2):414–416. doi: 10.1016/0005-2787(68)90095-6. [DOI] [PubMed] [Google Scholar]
- Nomura M., Lowry C. V. PHAGE f2 RNA-DIRECTED BINDING OF FORMYLMETHIONYL-TRNA TO RIBOSOMES AND THE ROLE OF 30S RIBOSOMAL SUBUNITS IN INITIATION OF PROTEIN SYNTHESIS. Proc Natl Acad Sci U S A. 1967 Sep;58(3):946–953. doi: 10.1073/pnas.58.3.946. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PETERS T., Jr Cytoplasmic particles and serum albumin synthesis. J Histochem Cytochem. 1959 Jul;7(4):224–234. doi: 10.1177/7.4.224. [DOI] [PubMed] [Google Scholar]
- PETERS T., Jr The biosynthesis of rat serum albumin. II. Intracellular phenomena in the secretion of newly formed albumin. J Biol Chem. 1962 Apr;237:1186–1189. [PubMed] [Google Scholar]
- Ragnotti G., Lawford G. R., Campbell P. N. Biosynthesis of microsomal nicotinamide-adenine dinucleotide phosphate-cytochrome c reductase by membrane-bound and free polysomes from rat liver. Biochem J. 1969 Apr;112(2):139–147. doi: 10.1042/bj1120139. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Redman C. M., Sabatini D. D. Vectorial discharge of peptides released by puromycin from attached ribosomes. Proc Natl Acad Sci U S A. 1966 Aug;56(2):608–615. doi: 10.1073/pnas.56.2.608. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Redman C. M. The synthesis of serum proteins on attached rather than free ribosomes of rat liver. Biochem Biophys Res Commun. 1968 Jun 28;31(6):845–850. doi: 10.1016/0006-291x(68)90528-7. [DOI] [PubMed] [Google Scholar]
- SIEKEVITZ P., PALADE G. E. A cytochemical study on the pancreas of the guinea pig. 5. In vivo incorporation of leucine-1-C14 into the chymotrypsinogen of various cell fractions. J Biophys Biochem Cytol. 1960 Jul;7:619–630. doi: 10.1083/jcb.7.4.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sabatini D. D., Tashiro Y., Palade G. E. On the attachment of ribosomes to microsomal membranes. J Mol Biol. 1966 Aug;19(2):503–524. doi: 10.1016/s0022-2836(66)80019-0. [DOI] [PubMed] [Google Scholar]
- Stecher V. J., Thorbecke G. J. Beta-1C and immune globulin formation in vitro by tissues from germ-free and conventional rodents of various ages. Immunology. 1967 Apr;12(4):475–487. [PMC free article] [PubMed] [Google Scholar]
- Takagi M., Ogata K. Direct evidence for albumin biosynthesis by membrane bound polysomes in rat liver. Biochem Biophys Res Commun. 1968 Oct 10;33(1):55–60. doi: 10.1016/0006-291x(68)90254-4. [DOI] [PubMed] [Google Scholar]
- WETTSTEIN F. O., STAEHELIN T., NOLL H. Ribosomal aggregate engaged in protein synthesis: characterization of the ergosome. Nature. 1963 Feb 2;197:430–435. doi: 10.1038/197430a0. [DOI] [PubMed] [Google Scholar]
- WILLIAMS C. A., GANOZA M. C., LIPMANN F. EFFECT OF BACTERIAL INFECTION ON THE SYNTHESIS OF SERUM PROTEINS BY A MOUSE LIVER CELL-FREE SYSTEM. Proc Natl Acad Sci U S A. 1965 Mar;53:622–626. doi: 10.1073/pnas.53.3.622. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WILLIAMS C. A., Jr, ASOFSKY R., THORBECKE G. J. PLASMA PROTEIN FORMATION IN VITRO BY TISSUES FROM MICE INFECTED WITH STAPHYLOCOCCI. J Exp Med. 1963 Aug 1;118:315–326. doi: 10.1084/jem.118.2.315. [DOI] [PMC free article] [PubMed] [Google Scholar]