Skip to main content
NCBI home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1969 Aug;63(4):1383–1388. doi: 10.1073/pnas.63.4.1383

TRANSFER RNA: A COMPARISON BY GEL ELECTROPHORESIS OF THE TRNA IN HELA CYTOPLASM, HELA MITOCHONDRIAL FRACTION, AND E. coli

E Knight Jr *, T Sugiyama *
PMCID: PMC223476  PMID: 4901475

Abstract

A class of transfer RNA separable from the majority of transfer RNA by gel electrophoresis has been observed in E. coli and in the mitochondrial fraction of HeLa cells but not in HeLa cytoplasm. The size of this class of transfer RNA, as judged by its mobility on polyacrylamide gel, is intermediate between the major part of the transfer RNA and 5S RNA. It exists in part associated with the ribosomes, and its amino acid acceptor activity is different from the major class of transfer RNA.

Full text

PDF
1383

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aubert M., Scott J. F., Reynier M., Monier R. Rearrangement of the conformation of Escherichia coli 5S RNA. Proc Natl Acad Sci U S A. 1968 Sep;61(1):292–299. doi: 10.1073/pnas.61.1.292. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barnett W. E., Brown D. H. Mitochondrial transfer ribonucleic acids. Proc Natl Acad Sci U S A. 1967 Feb;57(2):452–458. doi: 10.1073/pnas.57.2.452. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dubin D. T., Brown R. E. A novel ribosomal RNA in hamster cell mitochondria. Biochim Biophys Acta. 1967 Sep 26;145(2):538–540. doi: 10.1016/0005-2787(67)90080-9. [DOI] [PubMed] [Google Scholar]
  4. Dure L. S., Epler J. L., Barnett W. E. Sedimentation properties of mitochondrial and cytoplasmic ribosomal RNA's from Neurospora. Proc Natl Acad Sci U S A. 1967 Nov;58(5):1883–1887. doi: 10.1073/pnas.58.5.1883. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Galper J. B., Darnell J. E. The presence of N-formyl-methionyl-tRNA in HeLa cell mitochondria. Biochem Biophys Res Commun. 1969 Jan 27;34(2):205–214. doi: 10.1016/0006-291x(69)90633-0. [DOI] [PubMed] [Google Scholar]
  6. Küntzel H., Noll H. Mitochondrial and cytoplasmic polysomes from Neurospora crassa. Nature. 1967 Sep 23;215(5108):1340–1345. doi: 10.1038/2151340a0. [DOI] [PubMed] [Google Scholar]
  7. Leighton F., Poole B., Beaufay H., Baudhuin P., Coffey J. W., Fowler S., De Duve C. The large-scale separation of peroxisomes, mitochondria, and lysosomes from the livers of rats injected with triton WR-1339. Improved isolation procedures, automated analysis, biochemical and morphological properties of fractions. J Cell Biol. 1968 May;37(2):482–513. doi: 10.1083/jcb.37.2.482. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Loehr J. S., Keller E. B. Dimers of alanine transfer RNA with acceptor activity. Proc Natl Acad Sci U S A. 1968 Nov;61(3):1115–1122. doi: 10.1073/pnas.61.3.1115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Loening U. E. The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochem J. 1967 Jan;102(1):251–257. doi: 10.1042/bj1020251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rifkin M. R., Wood D. D., Luck D. J. Ribosomal RNA and ribosomes from mitochondria of Neurospora crassa. Proc Natl Acad Sci U S A. 1967 Sep;58(3):1025–1032. doi: 10.1073/pnas.58.3.1025. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Smith A. E., Marcker K. A. N-formylmethionyl transfer RNA in mitochondria from yeast and rat liver. J Mol Biol. 1968 Dec 14;38(2):241–243. doi: 10.1016/0022-2836(68)90409-9. [DOI] [PubMed] [Google Scholar]
  12. Sugiyama T., Nakada D. Control of translation of MS2 RNA cistrons by MS2 coat protein. Proc Natl Acad Sci U S A. 1967 Jun;57(6):1744–1750. doi: 10.1073/pnas.57.6.1744. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. TAMAOKI T., MUELLER G. C. Synthesis of nuclear and cytoplasmic RNA of HeLa cells and the effect of actinomycin D. Biochem Biophys Res Commun. 1962 Nov 27;9:451–454. doi: 10.1016/0006-291x(62)90033-5. [DOI] [PubMed] [Google Scholar]
  14. Vesco C., Penman S. The cytoplasmic RNA of HeLa cells: new discrete species associated with mitochondria. Proc Natl Acad Sci U S A. 1969 Jan;62(1):218–225. doi: 10.1073/pnas.62.1.218. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES