Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1969 Jul;63(3):724–731. doi: 10.1073/pnas.63.3.724

REGULATION OF SUGAR TRANSPORT IN ISOLATED BACTERIAL MEMBRANE PREPARATIONS FROM Escherichia coli

H R Kaback 1
PMCID: PMC223512  PMID: 4899873

Abstract

The experiments presented in this paper demonstrate that glucose transport via the phosphoenolpyruvate-phosphotransferase system as studied in isolated E. coli membrane preparations is subject to regulation by sugar-P's. Glucose transport in these preparations is inhibited by glucose-6-P, glucose-1-P, and, to a lesser extent, by other P-esters. There appear to be two inhibitory sites, one specific for glucose-1-P and related sugar-1-P's and the other specific for glucose-6-P and related sugar-6-P's. The inhibition by these sugar-P's is noncompetitive, and the inhibitory sites are separate, subject to independent control, and apparently are accessible from outside the membrane. Furthermore, glucose-6-P is able to reverse partially inhibition of glucose transport by glucose-1-P and vice versa. Preliminary evidence is presented indicating that glucose-1-P may play a central role in the regulation of sugar transport in general, by virtue of its ability to inhibit selectively the transport of sugars other than glucose.

Full text

PDF
724

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. KUNDIG W., GHOSH S., ROSEMAN S. PHOSPHATE BOUND TO HISTIDINE IN A PROTEIN AS AN INTERMEDIATE IN A NOVEL PHOSPHO-TRANSFERASE SYSTEM. Proc Natl Acad Sci U S A. 1964 Oct;52:1067–1074. doi: 10.1073/pnas.52.4.1067. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Kaback H. R., Deuel F. Proline uptake by disrupted membrane preparations from Escherichia coli. Arch Biochem Biophys. 1969 Jun;132(1):118–129. doi: 10.1016/0003-9861(69)90343-9. [DOI] [PubMed] [Google Scholar]
  3. Kaback H. R., Kostellow A. B. Glycine uptake in Escherichia coli. I. Glycine uptake by whole cells of Escherichia coli W+ and a D-serine-resistant. J Biol Chem. 1968 Apr 10;243(7):1384–1389. [PubMed] [Google Scholar]
  4. Kaback H. R., Stadtman E. R. Glycine uptake in Escherichia coli. II. Glycine uptake, exchange, and metabolism by an isolated membrane preparation. J Biol Chem. 1968 Apr 10;243(7):1390–1400. [PubMed] [Google Scholar]
  5. Kaback H. R., Stadtman E. R. Proline uptake by an isolated cytoplasmic membrane preparation of Escherichia coli. Proc Natl Acad Sci U S A. 1966 Apr;55(4):920–927. doi: 10.1073/pnas.55.4.920. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kaback H. R. The role of the phosphoenolpyruvate-phosphotransferase system in the transport of sugars by isolated membrane preparations of Escherichia coli. J Biol Chem. 1968 Jul 10;243(13):3711–3724. [PubMed] [Google Scholar]
  7. Loomis W. F., Jr, Magasanik B. Glucose-lactose diauxie in Escherichia coli. J Bacteriol. 1967 Apr;93(4):1397–1401. doi: 10.1128/jb.93.4.1397-1401.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Stadtman E. R., Shapiro B. M., Kingdon H. S., Woolfolk C. A., Hubbard J. S. Cellular regulation of glutamine synthetase activity in Escherichia coli. Adv Enzyme Regul. 1968;6:257–289. doi: 10.1016/0065-2571(68)90017-4. [DOI] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES