Abstract
The experiments presented in this paper demonstrate that glucose transport via the phosphoenolpyruvate-phosphotransferase system as studied in isolated E. coli membrane preparations is subject to regulation by sugar-P's. Glucose transport in these preparations is inhibited by glucose-6-P, glucose-1-P, and, to a lesser extent, by other P-esters. There appear to be two inhibitory sites, one specific for glucose-1-P and related sugar-1-P's and the other specific for glucose-6-P and related sugar-6-P's. The inhibition by these sugar-P's is noncompetitive, and the inhibitory sites are separate, subject to independent control, and apparently are accessible from outside the membrane. Furthermore, glucose-6-P is able to reverse partially inhibition of glucose transport by glucose-1-P and vice versa. Preliminary evidence is presented indicating that glucose-1-P may play a central role in the regulation of sugar transport in general, by virtue of its ability to inhibit selectively the transport of sugars other than glucose.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- KUNDIG W., GHOSH S., ROSEMAN S. PHOSPHATE BOUND TO HISTIDINE IN A PROTEIN AS AN INTERMEDIATE IN A NOVEL PHOSPHO-TRANSFERASE SYSTEM. Proc Natl Acad Sci U S A. 1964 Oct;52:1067–1074. doi: 10.1073/pnas.52.4.1067. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kaback H. R., Deuel F. Proline uptake by disrupted membrane preparations from Escherichia coli. Arch Biochem Biophys. 1969 Jun;132(1):118–129. doi: 10.1016/0003-9861(69)90343-9. [DOI] [PubMed] [Google Scholar]
- Kaback H. R., Kostellow A. B. Glycine uptake in Escherichia coli. I. Glycine uptake by whole cells of Escherichia coli W+ and a D-serine-resistant. J Biol Chem. 1968 Apr 10;243(7):1384–1389. [PubMed] [Google Scholar]
- Kaback H. R., Stadtman E. R. Glycine uptake in Escherichia coli. II. Glycine uptake, exchange, and metabolism by an isolated membrane preparation. J Biol Chem. 1968 Apr 10;243(7):1390–1400. [PubMed] [Google Scholar]
- Kaback H. R., Stadtman E. R. Proline uptake by an isolated cytoplasmic membrane preparation of Escherichia coli. Proc Natl Acad Sci U S A. 1966 Apr;55(4):920–927. doi: 10.1073/pnas.55.4.920. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kaback H. R. The role of the phosphoenolpyruvate-phosphotransferase system in the transport of sugars by isolated membrane preparations of Escherichia coli. J Biol Chem. 1968 Jul 10;243(13):3711–3724. [PubMed] [Google Scholar]
- Loomis W. F., Jr, Magasanik B. Glucose-lactose diauxie in Escherichia coli. J Bacteriol. 1967 Apr;93(4):1397–1401. doi: 10.1128/jb.93.4.1397-1401.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stadtman E. R., Shapiro B. M., Kingdon H. S., Woolfolk C. A., Hubbard J. S. Cellular regulation of glutamine synthetase activity in Escherichia coli. Adv Enzyme Regul. 1968;6:257–289. doi: 10.1016/0065-2571(68)90017-4. [DOI] [PubMed] [Google Scholar]