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. 1969 Jul;63(3):724–731. doi: 10.1073/pnas.63.3.724

REGULATION OF SUGAR TRANSPORT IN ISOLATED BACTERIAL MEMBRANE PREPARATIONS FROM Escherichia coli

H R Kaback 1
PMCID: PMC223512  PMID: 4899873

Abstract

The experiments presented in this paper demonstrate that glucose transport via the phosphoenolpyruvate-phosphotransferase system as studied in isolated E. coli membrane preparations is subject to regulation by sugar-P's. Glucose transport in these preparations is inhibited by glucose-6-P, glucose-1-P, and, to a lesser extent, by other P-esters. There appear to be two inhibitory sites, one specific for glucose-1-P and related sugar-1-P's and the other specific for glucose-6-P and related sugar-6-P's. The inhibition by these sugar-P's is noncompetitive, and the inhibitory sites are separate, subject to independent control, and apparently are accessible from outside the membrane. Furthermore, glucose-6-P is able to reverse partially inhibition of glucose transport by glucose-1-P and vice versa. Preliminary evidence is presented indicating that glucose-1-P may play a central role in the regulation of sugar transport in general, by virtue of its ability to inhibit selectively the transport of sugars other than glucose.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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