Abstract
The formation of trifluoroleucine-tRNA and leucine-tRNA during embryonic development of the mouse is a function of gestational age. The existence of a leucine-tRNA synthetase has been demonstrated in early embryonic tissue (gestational age <10 days) which has unique chemical properties, acylating tRNA with trifluoroleucine with avidity almost equal to leucine. This enzyme has been shown to be stable to heating at 45°C for 30 minutes.
A second leucine-tRNA synthetase, which is similar chemically and physically to the enzyme isolated from more mature embryonic tissue, has been isolated from this embyronic source as well as from adult tissue.
Quantitative changes in the species of tRNA isolated from these various sources have been documented utilizing the technique of polyacrylamide electrophoresis. These changes parallel the disappearance of the early embryonic enzyme, fewer species of tRNA acting as acceptors for leucine as maturation progresses.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- DOCTOR B. P., MUDD J. A. SPECIES SPECIFICITY OF AMINO ACID ACCEPTOR RIBONUCLEIC ACID AND AMINOACYL SOLUBLE RIBONUCLEIC ACID SYNTHETASES. J Biol Chem. 1963 Nov;238:3677–3681. [PubMed] [Google Scholar]
- EIDLIC L., NEIDHARDT F. C. PROTEIN AND NUCLEIC ACID SYNTHESIS IN TWO MUTANTS OF ESCHERICHIA COLI WITH TEMPERATURE-SENSITIVE AMINOACYL RIBONUCLEIC ACID SYNTHETASES. J Bacteriol. 1965 Mar;89:706–711. doi: 10.1128/jb.89.3.706-711.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
- FANGMAN W. L., NEIDHARDT F. C. PROTEIN AND RIBONUCLEIC ACID SYNTHESIS IN A MUTANT OF ESCHERICHIA COLI WITH AN ALTERED AMINOACYL RIBONUCLEIC ACID SYNTHETASE. J Biol Chem. 1964 Jun;239:1844–1847. [PubMed] [Google Scholar]
- Lee J. C., Ingram V. M. Erythrocyte transfer RNA: change during chick development. Science. 1967 Dec 8;158(3806):1330–1332. doi: 10.1126/science.158.3806.1330. [DOI] [PubMed] [Google Scholar]
- Neidhardt F. C. Roles of amino acid activating enzymes in cellular physiology. Bacteriol Rev. 1966 Dec;30(4):701–719. doi: 10.1128/br.30.4.701-719.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
- PROFFIT W. R., EDWARDS L. E. Effects of ethionine administration during pregnancy in the rat. J Exp Zool. 1962 Jul;150:135–142. doi: 10.1002/jez.1401500207. [DOI] [PubMed] [Google Scholar]
- RENNERT O. M., ANKER H. S. EFFECT OF 5',5',5'-TRIFLUOROLEUCINE ON A NUMBER OF MOUSE LEUKAEMIAS. Nature. 1964 Sep 19;203:1256–1257. doi: 10.1038/2031256a0. [DOI] [PubMed] [Google Scholar]
- RENNERT O. M., ANKER H. S. ON THE INCORPORATION OF 5',5',5'-TRIFLUOROLEUCINE INTO PROTEINS OF E. COLI. Biochemistry. 1963 May-Jun;2:471–476. doi: 10.1021/bi00903a013. [DOI] [PubMed] [Google Scholar]
- ROSENBAUM M., BROWN R. A. Simplified preparation and properties of undergraded rat liver transfer ribonucleic acid. Anal Biochem. 1961 Feb;2:15–22. doi: 10.1016/0003-2697(61)90034-3. [DOI] [PubMed] [Google Scholar]
- Strehler B. L., Hendley D. D., Hirsch G. P. Evidence of a codon restriction hypothesis of cellular differentiation: multiplicity of mammalian leucyl-sRNA-specific synthetases and tissue-specific deficiency in an alanyl-sRNA synthetase. Proc Natl Acad Sci U S A. 1967 Jun;57(6):1751–1758. doi: 10.1073/pnas.57.6.1751. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vold B. S., Sypherd P. S. Modification in transfer RNA during the differentiation of wheat seedlings. Proc Natl Acad Sci U S A. 1968 Feb;59(2):453–458. doi: 10.1073/pnas.59.2.453. [DOI] [PMC free article] [PubMed] [Google Scholar]