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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1969 Apr;62(4):1093–1099. doi: 10.1073/pnas.62.4.1093

EVENTS OCCURRING DURING THE REPLICATION OF BACTERIOPHAGE T4 DNA*

Paul D Scotti 1
PMCID: PMC223619  PMID: 4894686

Abstract

Ten temperature-sensitive mutants of T4D have been examined to find the times in the lytic cycle at which a shift to a restrictive temperature no longer exerts a lethal effect. Nine of these mutants play a role in phage DNA synthesis. The results of these experiments suggest that the products of three of the genes tested—genes 42, 44, and 45—have control or complexing functions in addition to any enzymatic ones. The data also indicate that the products of genes 30 and 41 may act cooperatively. The properties of mutants of gene 43 (DNA polymerase) and gene 56 (dCTP-dUTPase) suggest that the production of a certain length of concatameric DNA is necessary before viable phage can be produced.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. De Waard A., Paul A. V., Lehman I. R. The structural gene for deoxyribonucleic acid polymerase in bacteriophages T4 and T5. Proc Natl Acad Sci U S A. 1965 Oct;54(4):1241–1248. doi: 10.1073/pnas.54.4.1241. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fareed G. C., Richardson C. C. Enzymatic breakage and joining of deoxyribonucleic acid. II. The structural gene for polynucleotide ligase in bacteriophage T4. Proc Natl Acad Sci U S A. 1967 Aug;58(2):665–672. doi: 10.1073/pnas.58.2.665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hosoda J., Levinthal C. Protein synthesis by Escherichia coli infected with bacteriophage T4D. Virology. 1968 Apr;34(4):709–727. doi: 10.1016/0042-6822(68)90092-5. [DOI] [PubMed] [Google Scholar]
  4. Oishi M. Studies of DNA replication in vivo. 3. Accumulation of a single-stranded isolation product of DNA replication by conditional mutant strains of T4. Proc Natl Acad Sci U S A. 1968 Jul;60(3):1000–1006. doi: 10.1073/pnas.60.3.1000. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Streisinger G., Emrich J., Stahl M. M. Chromosome structure in phage t4, iii. Terminal redundancy and length determination. Proc Natl Acad Sci U S A. 1967 Feb;57(2):292–295. doi: 10.1073/pnas.57.2.292. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Warner H. R., Barnes J. E. Deoxyribonucleic acid synthesis in Escherichia coli infected with some deoxyribonucleic acid polymerase-less mutants of bacteriophage T4. Virology. 1966 Jan;28(1):100–107. doi: 10.1016/0042-6822(66)90310-2. [DOI] [PubMed] [Google Scholar]
  7. Warner H. R., Barnes J. E. Evidence for a dual role for the bacteriophage T4-induced deoxycytidine triphosphate nucleotidohydrolase. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1233–1240. doi: 10.1073/pnas.56.4.1233. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Warner H. R., Hobbs M. D. Incorporation of uracil-14C into nucleic acids in Escherichia coli infected with bacteriophage T4 and T4 amber mutants. Virology. 1967 Nov;33(3):376–384. doi: 10.1016/0042-6822(67)90113-4. [DOI] [PubMed] [Google Scholar]
  9. Werner R. Distribution of growing points in DNa of bacteriophage T4. J Mol Biol. 1968 May 14;33(3):679–692. doi: 10.1016/0022-2836(68)90313-6. [DOI] [PubMed] [Google Scholar]

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