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. 1969 Apr;62(4):1203–1209. doi: 10.1073/pnas.62.4.1203

TRANSFER RNA, III. RECONSTITUTION OF ALANINE ACCEPTOR ACTIVITY FROM FRAGMENTS PRODUCED BY SPECIFIC CLEAVAGE OF tRNAAlaII AT ITS ANTICODON*,†

Nobumasa Imura 1,‡, Harvey Schwam 1,§, Robert W Chambers 1,**
PMCID: PMC223634  PMID: 5256416

Abstract

This report describes experiments showing that tRNAAlaII can be cleaved specifically at the G residue of its anticodon to give “half” molecules in high yield. Neither of these fragments has alanine-acceptor activity, but this activity can be reconstituted by mixing the fragments in the presence of Mg+2. In dilute salt, on the other hand, the active complex dissociates spontaneously at 25° into the two fragments. Thus, both “halves” of tRNAAlaII are necessary for acceptor activity and, in the presence of Mg+2, combination of these fragments to give an active duplex is thermodynamically favorable.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chang S. H., RajBhandary U. L. Studies on polynucleotides. LXXXI. Yeast phenylalanine transfer ribonucleic acid: partial digestion with pancreatic ribonuclease. J Biol Chem. 1968 Feb 10;243(3):592–597. [PubMed] [Google Scholar]
  2. Gillam I., Millward S., Blew D., von Tigerstrom M., Wimmer E., Tener G. M. The separation of soluble ribonucleic acids on benzoylated diethylaminoethylcellulose. Biochemistry. 1967 Oct;6(10):3043–3056. doi: 10.1021/bi00862a011. [DOI] [PubMed] [Google Scholar]
  3. HOLLEY R. W., APGAR J., EVERETT G. A., MADISON J. T., MARQUISEE M., MERRILL S. H., PENSWICK J. R., ZAMIR A. STRUCTURE OF A RIBONUCLEIC ACID. Science. 1965 Mar 19;147(3664):1462–1465. doi: 10.1126/science.147.3664.1462. [DOI] [PubMed] [Google Scholar]
  4. Reeves R. H., Imura N., Schwam H., Weiss G. B., Schulman L. H., Chambers R. W. Transfer RNA, I. Isolation and characterization of a new yeast alanine transfer RNA. Proc Natl Acad Sci U S A. 1968 Aug;60(4):1450–1457. doi: 10.1073/pnas.60.4.1450. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Scheffler I. E., Elson E. L., Baldwin R. L. Helix formation by dAT oligomers. I. Hairpin and straight-chain helices. J Mol Biol. 1968 Sep 28;36(3):291–304. doi: 10.1016/0022-2836(68)90156-3. [DOI] [PubMed] [Google Scholar]
  6. Schulman L. H., Chambers R. W. Transfer RNA, II. A structural basis for alanine acceptor activity. Proc Natl Acad Sci U S A. 1968 Sep;61(1):308–315. doi: 10.1073/pnas.61.1.308. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Tomasz M., Chambers R. W. The chemistry of pseudouridine. VII. Selective cleavage of polynucleotides containing pseudouridylic acid residues by a unique photochemical reaction. Biochemistry. 1966 Feb;5(2):773–782. doi: 10.1021/bi00866a052. [DOI] [PubMed] [Google Scholar]

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