Skip to main content
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1969 Apr;62(4):1218–1225. doi: 10.1073/pnas.62.4.1218

THE INTERACTION OF RNA POLYMERASE WITH HISTONES*

Thomas C Spelsberg 1, Sharon Tankersley 1, Lubomir S Hnilica 1
PMCID: PMC223636  PMID: 5256418

Abstract

Arginine-rich histones (F3) interact with both the bacterial and mammalian RNA polymerase and inhibit the in vitro RNA synthesis to a much greater extent than when associated with the DNA template. The lysine-rich histones (F1) inhibit the RNA synthesis mainly through template inhibition. Neither histone fraction displayed such an interaction with DNA polymerase. The RNA polymerase-F3 histone interaction takes place at ionic strength equal to or greater than those occurring in living cells, suggesting a possible role of arginine-rich histones in the regulation of RNA synthesis.

Full text

PDF
1218

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bonner J., Dahmus M. E., Fambrough D., Huang R. C., Marushige K., Tuan D. Y. The Biology of Isolated Chromatin: Chromosomes, biologically active in the test tube, provide a powerful tool for the study of gene action. Science. 1968 Jan 5;159(3810):47–56. doi: 10.1126/science.159.3810.47. [DOI] [PubMed] [Google Scholar]
  2. Cunningham D. D., Steiner D. F. Extraction of RNA polymerase from rat liver nuclei in a soluble form. Biochim Biophys Acta. 1967;145(3):834–836. doi: 10.1016/0005-2787(67)90144-x. [DOI] [PubMed] [Google Scholar]
  3. FURTH J. J., HO P. THE ENZYMATIC SYNTHESIS OF RIBONUCLEIC ACID IN ANIMAL TISSUE. I. THE DEOXYRIBONUCLEIC ACID-DIRECTED SYNTHESIS OF RIBONUCLEIC ACID AS CATALYZED BY AN ENZYME OBTAINED FROM BOVINE LYMPHOSARCOMA TISSUE. J Biol Chem. 1965 Jun;240:2602–2606. [PubMed] [Google Scholar]
  4. Farmbrough D. M., Fujimura F., Bonner J. Quantitative distribution of histone components in the pea plant. Biochemistry. 1968 Feb;7(2):575–585. doi: 10.1021/bi00842a010. [DOI] [PubMed] [Google Scholar]
  5. Georgiev G. P., Ananieva L. N., Kozlov J. V. Stepwise removal of protein from a deoxyribonucleoprotein complex and de-repression of the genome. J Mol Biol. 1966 Dec 28;22(2):365–371. doi: 10.1016/0022-2836(66)90140-9. [DOI] [PubMed] [Google Scholar]
  6. Hnilica L. S., Bess L. G. The heterogeneity of arginine-rich histones. Anal Biochem. 1965 Sep;12(3):421–436. doi: 10.1016/0003-2697(65)90209-5. [DOI] [PubMed] [Google Scholar]
  7. Hnilica L. S., Edwards L. J., Hey A. E. Studies on nuclear proteins. II. Quantitative distribution of histone fractions in various tissues. Biochim Biophys Acta. 1966 Jul 27;124(1):109–117. doi: 10.1016/0304-4165(66)90318-7. [DOI] [PubMed] [Google Scholar]
  8. Hnilica L. S. Studies on nuclear proteins. I. Observations on the tissue and species specificity of the moderately lysine-rich histone fraction 2b. Biochim Biophys Acta. 1966 Mar 28;117(1):163–175. doi: 10.1016/0304-4165(66)90163-2. [DOI] [PubMed] [Google Scholar]
  9. Jacob S. T., Sajdel E. M., Munro H. N. Altered characteristics of mammalian RNA polymerase following solubilization from nuclei. Biochem Biophys Res Commun. 1968 Sep 6;32(5):831–838. doi: 10.1016/0006-291x(68)90316-1. [DOI] [PubMed] [Google Scholar]
  10. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  11. Littau V. C., Burdick C. J., Allfrey V. G., Mirsky S. A. The role of histones in the maintenance of chromatin structure. Proc Natl Acad Sci U S A. 1965 Oct;54(4):1204–1212. doi: 10.1073/pnas.54.4.1204. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. NAKAMOTO T., FOX C. F., WEISS S. B. ENZYMATIC SYNTHESIS OF RIBONUCLEIC ACID. I. PREPARATION OF RIBONUCLEIC ACID POLYMERASE FROM EXTRACTS OF MICROCOCCUS LYSODEIKTICUS. J Biol Chem. 1964 Jan;239:167–174. [PubMed] [Google Scholar]
  13. STEDMAN E. Cell specificity of histones. Nature. 1950 Nov 4;166(4227):780–781. doi: 10.1038/166780a0. [DOI] [PubMed] [Google Scholar]
  14. Seifart K., Sekeris C. E. Extraction of DNA-dependent RNA polymerase from rat liver nuclei. Hoppe Seylers Z Physiol Chem. 1967 Nov;348(11):1555–1557. [PubMed] [Google Scholar]
  15. Stellwagen R. H., Cole R. D. Danger of contamination in chromatographically prepared arginine-rich histone. J Biol Chem. 1968 Sep 10;243(17):4452–4455. [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES