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. 1969 Mar;62(3):636–643. doi: 10.1073/pnas.62.3.636

PROTON MAGNETIC RESONANCE STUDIES OF PORPHYRIN IRON (III) CYANIDES*

K Wüthrich 1,2, R G Shulman 1,2, B J Wyluda 1,2, W S Caughey 1,2
PMCID: PMC223644  PMID: 5256994

Abstract

The nuclear magnetic resonance (NMR) spectra at 220 Mc of cyanodeuteroporphyrin IX dimethyl ester iron (III), cyanoprotoporphyrin IX diethyl ester iron (III), and cyanoprotoporphyrin IX iron (III) in a mixed solvent of 80 per cent d5-pyridine and 20 per cent D2O have been measured. The resonances are assigned and, except for the ester alkoxy groups, are shown to be shifted by temperature-dependent hyperfine interactions with Fe (III). From the experiments, upper limits are placed upon the pseudocontact contributions, and values of the contact interactions are derived. Spin densities in carbon π-orbitals are calculated from the contact shifts and discussed in terms of preliminary Hückel calculations.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alben J. O., Fuchsman W. H., Beaudreau C. A., Caughey W. S. Substituted deuteroporphyrins. 3. Iron (II) derivatives. Reactions with oxygen and preparations from chloro- and methoxohemins. Biochemistry. 1968 Feb;7(2):624–635. doi: 10.1021/bi00842a018. [DOI] [PubMed] [Google Scholar]
  2. CAUGHEY W. S., KOSKI W. S. Nuclear magnetic resonance spectra of porphyrins. Biochemistry. 1962 Sep;1:923–931. doi: 10.1021/bi00911a028. [DOI] [PubMed] [Google Scholar]
  3. GIBSON J. F., INGRAM D. J. Electron resonance studies of haemoglobin azide and hydroxide derivatives. Nature. 1957 Jul 6;180(4575):29–30. doi: 10.1038/180029a0. [DOI] [PubMed] [Google Scholar]
  4. GRIFFITH J. S. Theory of electron resonance in ferrihaemoglobin azide. Nature. 1957 Jul 6;180(4575):30–31. doi: 10.1038/180030a0. [DOI] [PubMed] [Google Scholar]
  5. Kurland R. J., Davis D. G., Ho C. Paramagnetic proton nuclear magnetic resonance shifts of metmyoglobin, methemoglobin, and hemin derivatives. J Am Chem Soc. 1968 May 8;90(10):2700–2701. doi: 10.1021/ja01012a048. [DOI] [PubMed] [Google Scholar]
  6. Wüthrich K., Shulman R. G., Peisach J. High-resolution proton magnetic resonance spectra of sperm whale cyanometmyoglobin. Proc Natl Acad Sci U S A. 1968 Jun;60(2):373–380. doi: 10.1073/pnas.60.2.373. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Wüthrich K., Shulman R. G., Yamane T. Proton magnetic resonance studies of human cyanomethemoglobin. Proc Natl Acad Sci U S A. 1968 Dec;61(4):1199–1206. doi: 10.1073/pnas.61.4.1199. [DOI] [PMC free article] [PubMed] [Google Scholar]

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